Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment

1989 ◽  
Vol 8 (8) ◽  
pp. 429-432 ◽  
Author(s):  
K. K. Kartha ◽  
R. N. Chibbar ◽  
F. Georges ◽  
N. Leung ◽  
K. Caswell ◽  
...  
Keyword(s):  
Transient Expression ◽  
Cell Cultures ◽  
Microprojectile Bombardment ◽  
Chloramphenicol Acetyltransferase ◽  
Immature Embryos ◽  
Barley Cell ◽  
Cat Gene

Initiation and morphological development of somatic embryoids from barley cell cultures

1984 ◽  
Vol 62 (6) ◽  
pp. 1245-1249 ◽  
Author(s):  
L. S. Kott ◽  
K. J. Kasha
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Cell Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Morphological Development ◽  
Low Concentrations ◽  
The Media ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Barley Cell

Somatic embryogenesis was induced in callus previously initiated from immature embryos of barley. These cultures ranged in age from 6 weeks to 30 months. Embryoids were readily initiated from homogenized suspension-grown aggregates when plated on modified B5 media with 2,4-dichlorophenoxyacetic acid. Low concentrations (0.1 and 0.05 mg∙L−1) of abscisic acid promoted further maturation of embryoids, while gibberellic acid (1 mg∙L−1) and kinetin (0.1 mg∙L−1) were used in the media to encourage embryoid germination. The development of somatic embryoids from initiation through maturation and germination is described.

Generation of infectious Moloney murine leukemia viruses with deletions in the U3 portion of the long terminal repeat

1986 ◽  
Vol 6 (12) ◽  
pp. 4634-4640
Author(s):  
R Hanecak ◽  
S Mittal ◽  
B R Davis ◽  
H Fan
Keyword(s):  
Long Terminal Repeat ◽  
Transient Expression ◽  
Leukemia Virus ◽  
Chloramphenicol Acetyltransferase ◽  
Terminal Repeat ◽  
Tata Box ◽  
Moloney Murine Leukemia Virus ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Leukemia Viruses ◽  
Murine Leukemia

Deletional analysis within the long terminal repeat (LTR) of Moloney murine leukemia virus (M-MuLV) was performed. By molecular cloning, deletions were made in the vicinity of the XbaI site at -150 base pairs (bp) in the U3 region, between the tandemly repeated enhancers and the TATA box. The effects of the deletions on LTR function were measured in two ways. First, deleted LTRs were fused to the bacterial chloramphenicol acetyltransferase gene and used in transient expression assays. Second, infectious M-MuLVs were generated by transfection of M-MuLV proviruses containing the deleted LTRs, and the relative infectivity of the mutant viruses was assessed by XC-syncytial assay. Most of the deleted LTRs examined showed relatively high promoter activity in the transient chloramphenicol acetyltransferase assays, with values ranging from 20 to 50% of the wild-type M-MuLV LTR. Thus, the sequences between the enhancers and the TATA box were not absolutely required for transient expression. However, infectivity of viruses carrying the same deleted LTRs showed more pronounced effects. Deletion of sequences from -195 to -174 bp reduced infectivity 20- to 100-fold. Deletion of sequences within the region from -174 to -122 bp did not affect infectivity, indicating that this region is dispensable. On the other hand, deletion of sequences from -150 to -40 bp reduced infectivity from 5 to 6 logs, although the magnitude of the reduction partly may have reflected threshold envelope protein requirements for positive XC assays. The reduced infectivity did not appear to result from a failure of proviral DNA synthesis or integration by the mutant. Thus, the infectivity measurements identified three functional domains in the region between the enhancers and the TATA box.

Transient expression of marker genes in immature zygotic embryos of spring wheat (Triticum aestivum) through microprojectile bombardment

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 453-460 ◽  
Author(s):  
R. N. Chibbar ◽  
K. K. Kartha ◽  
N. Leung ◽  
J. Qureshi ◽  
K. Caswell
Keyword(s):  
Triticum Aestivum ◽  
Transient Expression ◽  
Microprojectile Bombardment ◽  
Triticum Aestivum L ◽  
Cauliflower Mosaic Virus ◽  
Expression Vectors ◽  
Marker Genes ◽  
Zygotic Embryos ◽  
Coding Region ◽  
Immature Zygotic Embryos

Transient expression of marker genes (cat and uidA) delivered by the Biolistics microprojectile bombardment technique has been detected in immature zygotic embryos of wheat (Triticum aestivum L.). The DNA expression vectors that gave maximal expression of both cat (pCaMVI1CN) and uidA (pCaMVI1GusN) genes had an alcohol dehydrogenase (Adh1) intron 1 cloned in between the cauliflower mosaic virus (CaMV35S) promoter and the coding region of the gene. Detection of chloramphenicol acetyltransferase (CAT) activity in response to cat gene was complicated by the presence of an inhibitor of CAT activity as well as an endogenous CAT-Iike activity. The results of enzymatic assays were confirmed by an ELISA technique using CAT-specific antibodies, whereas the β-glucuronidase (GUS) activity following the introduction of the uidA gene was confirmed by both histochemical and fluorometric techniques.Key words: cereal transformation, gene expression, ELISA, wheat.

scholarly journals Transient expression of green fluorescent protein in various plastid types following microprojectile bombardment

1998 ◽  
Vol 16 (5) ◽  
pp. 627-632 ◽  
Author(s):  
Julian M. Hibberd ◽  
Philip J. Linley ◽  
Muhammad S. Khan ◽  
John C. Gray
Keyword(s):  
Green Fluorescent Protein ◽  
Transient Expression ◽  
Fluorescent Protein ◽  
Microprojectile Bombardment ◽  
Green Fluorescent

scholarly journals Biolistic and Agrobacterium-mediated transient expression of UidA in Triticale immature embryos

2012 ◽  
Vol 41 (Special Issue) ◽  
pp. 228-232 ◽  
Author(s):  
S. Rubio ◽  
N. Jouve ◽  
M. González J
Keyword(s):  
Transient Expression ◽  
Full Text ◽  
Immature Embryos

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scholarly journals Arginine-mediated regulation of an argininosuccinate synthetase minigene in normal and canavanine-resistant human cells.

1986 ◽  
Vol 6 (6) ◽  
pp. 2257-2261 ◽  
Author(s):  
M J Jackson ◽  
W E O'Brien ◽  
A L Beaudet
Keyword(s):  
Gene Expression ◽  
Chloramphenicol Acetyltransferase ◽  
Human Cells ◽  
Argininosuccinate Synthetase ◽  
Cat Gene ◽  
Metabolite Regulation ◽  
In Cells

Regulation of argininosuccinate synthetase (AS) was studied by using minigenes containing 3 kilobases of DNA upstream from the TATAA box and 9 kilobases downstream (including the first four exons of the AS gene) ligated to either the cDNA for AS or to the chloramphenicol acetyltransferase (CAT) gene. Unlike the endogenous AS gene, expression of the CAT minigene was not elevated in Canr1 cells, which overproduce AS compared with parental RPMI-2650 cells. Expression of the CAT minigene in both stable and transient analyses was four- to five-fold higher in RPMI-2650 cells grown in citrulline medium than in cells grown in arginine medium. Although endogenous AS activity is not subject to metabolite regulation in Canr1 cells and expression of the CAT minigene in Canr1 cells was not increased when cells were grown in citrulline medium, expression of the CAT minigene was 10- to 22-fold greater when intracellular arginine pools were depleted by transient starvation for arginine and citrulline.

The low level expression of chloramphenicol acetyltransferase (CAT) mRNA in Escherichia coli is not dependent on either Shine-Dalgarno or the downstream boxes in the CAT gene

1998 ◽  
Vol 153 (2) ◽  
pp. 173-178 ◽  
Author(s):  
M. Odjakova ◽  
A. Golshani ◽  
G. Ivanov ◽  
M. Abou Haidar ◽  
I. Ivanov
Keyword(s):  
Escherichia Coli ◽  
Chloramphenicol Acetyltransferase ◽  
Low Level ◽  
Cat Gene

scholarly journals Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos

1999 ◽  
Vol 99 (3-4) ◽  
pp. 437-444 ◽  
Author(s):  
N. Bohorova ◽  
W. Zhang ◽  
P. Julstrum ◽  
S. McLean ◽  
B. Luna ◽  
...  
Keyword(s):  
Microprojectile Bombardment ◽  
Immature Embryos ◽  
Tropical Maize

scholarly journals Follicle-Stimulating Hormone Induces Transient Expression of the Protooncogene c-fos in Primary Sertoli Cell Cultures

1988 ◽  
Vol 2 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Susan H. Hair ◽  
David R. Joseph ◽  
Frank S. French ◽  
Marco Conti
Keyword(s):  
Sertoli Cell ◽  
Transient Expression ◽  
Cell Cultures ◽  
Follicle Stimulating Hormone ◽  
Stimulating Hormone
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