Ultrastructural and immunocytochemical analysis of the circumferential microfilament bundle in avian retinal pigmented epithelial cells in vitro

1991 ◽  
Vol 263 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Ryuji Kodama ◽  
Goro Eguchi ◽  
Robert O. Kelley
Keyword(s):  
Epithelial Cells ◽  
Immunocytochemical Analysis ◽  
Microfilament Bundle ◽  
Retinal Pigmented Epithelial Cells

The response of human retinal pigmented epithelial cells in vitro to changes in nitric oxide concentration stimulated by low levels of red light

2013 ◽  
Author(s):  
Brent J. Lavey ◽  
Larry E. Estlack ◽  
Kurt J. Schuster ◽  
Benjamin A. Rockwell ◽  
Jeffrey C. Wigle
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Red Light ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
Retinal Pigmented Epithelial Cells ◽  
Low Levels

scholarly journals Near infrared light exposure is associated with increased mitochondrial membrane potential in retinal pigmented epithelial cells

2020 ◽  
Vol 19 (10) ◽  
pp. 1455-1459
Author(s):  
Catherine Rono ◽  
Tiffany R Oliver
Keyword(s):  
Membrane Potential ◽  
Epithelial Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Near Infrared ◽  
Light Exposure ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Retinal Pigmented Epithelial Cells

The goal of this study was to characterize the effect of near-infrared light exposure on mitochondrial membrane potential, in vitro.

Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro

Development ◽  
1993 ◽  
Vol 118 (4) ◽  
pp. 1025-1030
Author(s):  
K. Agata ◽  
H. Kobayashi ◽  
Y. Itoh ◽  
M. Mochii ◽  
K. Sawada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Genetic Characterization ◽  
Pigment Cells ◽  
Intermediate Cell ◽  
Lens Cells ◽  
Myc Gene ◽  
Retinal Pigmented Epithelial Cells ◽  
Testicular Hyaluronidase

Retinal pigmented epithelial cells (PECs) of chicken embryos extensively and almost synchronously transdifferentiate into lens cells in medium containing phenylthiourea and testicular hyaluronidase, passing through the bipotent dedifferentiated state. We have isolated genes that are expressed specifically by either pigment or lens cells and analyzed their expression in the transdifferentiation process. The expression of some proto-oncogenes was also studied. In the dedifferentiation process, expression of the c-myc gene was enhanced and the transcription of PEC-specific genes (MMP115, pP344) was completely repressed. However, transcription of lens-specific genes (alpha-, beta- and delta-crystallins genes) remained silent in dedifferentiated pigment cells. Activation of len- or PEC-specific genes occurred only in conditions permissive for lens or PEC differentiation, respectively. These results indicated that lens transdifferentiation from PECs proceeds through a multipotent (or at least bipotent) intermediate cell state in which the c-myc gene is activated, but neither PEC- nor lens-specific genes are expressed.

scholarly journals Characterization of an in vitro Model of Calcification in Retinal Pigmented Epithelial Cells

2003 ◽  
Vol 10 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Hideki Sugitani ◽  
Hiroshi Wachi ◽  
Hayato Murata ◽  
Fumiaki Sato ◽  
Robert P. Mecham ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigmented Epithelial Cells

Quick freeze-deep etch analysis of microfilaments in the circumferential bundle of retinal pigmented epithelial cells M vitro

1986 ◽  
Vol 44 ◽  
pp. 160-161
Author(s):  
Ryuji Kodama ◽  
Goro Eguchi ◽  
Robert O. Kelley
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Embryonic Chick ◽  
Rpe Cells ◽  
Chick Retina ◽  
Lens Cell ◽  
Culture Environment ◽  
Retinal Pigmented Epithelial Cells

Pigmented epithelial cells from embryonic chick retina can transdifferentiate in vitro to express either pigmented (RPE) or lens cell (LC) phenotypes in response to alterations in the external culture environment. These observations suggest that the dedifferentiated phenotype of RPE cells is, at least, bipotential and that expression of either of two differentiated phenotypes is mediated by the cell surface and the associated cytoskeleton.

scholarly journals Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration

2021 ◽  
Vol 12 ◽  
Author(s):  
Francesca Lazzara ◽  
Federica Conti ◽  
Chiara Bianca Maria Platania ◽  
Chiara M. Eandi ◽  
Filippo Drago ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Macular Degeneration ◽  
Vision Loss ◽  
Cell Damage ◽  
Retinal Disease ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Retinal Pigmented Epithelial Cells

Age-related macular degeneration (AMD) is a degenerative retinal disease and one of major causes of irreversible vision loss. AMD has been linked to several pathological factors, such as oxidative stress and inflammation. Moreover, Aβ (1–42) oligomers have been found in drusen, the extracellular deposits that accumulate beneath the retinal pigmented epithelium in AMD patients. Hereby, we investigated the hypothesis that treatment with 1,25(OH) 2D3 (vitamin D3) and meso-zeaxathin, physiologically present in the eye, would counteract the toxic effects of three different insults on immortalized human retinal pigmented epithelial cells (ARPE-19). Specifically, ARPE-19 cells have been challenged with Aβ (1–42) oligomers, H2O2, LPS, and TNF-α, respectively. In the present study, we demonstrated that the combination of 1,25(OH)2D3 and meso-zeaxanthin significantly counteracted the cell damage induced by the three insults, at least in these in vitro integrated paradigms of AMD. These results suggest that combination of 1,25(OH)2D3 and meso-zeaxathin could be a useful approach to contrast pathological features of AMD, such as retinal inflammation and oxidative stress.

Adhesiveness and distribution of vinculin and spectrin in retinal pigmented epithelial cells during growth and differentiation in vitro

1985 ◽  
Vol 107 (2) ◽  
pp. 269-280 ◽  
Author(s):  
Michal Opas ◽  
Kursad Turksen ◽  
Vitauts I. Kalnins
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigmented Epithelial Cells

scholarly journals Role of integrins in differentiation of chick retinal pigmented epithelial cells in vitro

1996 ◽  
Vol 38 (4) ◽  
pp. 429-437 ◽  
Author(s):  
Yuichi Mazaki ◽  
Makoto Mochii ◽  
Ryuji Kodama ◽  
Goro Eguchi
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigmented Epithelial Cells

AN IN VITRO MODEL OF OXIDATIVE STRESS AND HYPOXIA IN RETINAL PIGMENTED EPITHELIAL CELLS

2001 ◽  
Vol 20 (1) ◽  
pp. 39-47
Author(s):  
M. Palmero ◽  
J. L. Bellot ◽  
C. Garcia-Cabanes ◽  
A. Orts
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
In Vitro Model ◽  
Retinal Pigmented Epithelial Cells ◽  
Vitro Model
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