Radio-frequency glow discharge ion source for high resolution mass spectrometry

1995 ◽  
Vol 353 (5-8) ◽  
pp. 570-574 ◽  
Author(s):  
A. I. Saprykin ◽  
F.-G. Melchers ◽  
J. S. Becker ◽  
H.-J. Dietze
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Glow Discharge ◽  
Radio Frequency ◽  
High Resolution Mass Spectrometry ◽  
Ion Source ◽  
Radio Frequency Glow Discharge ◽  
High Resolution Mass ◽  
Resolution Mass

ISOTOPIC ANOMALIES IN A URANIUM CLUSTER FORMED BY LAIS

1999 ◽  
Vol 06 (03n04) ◽  
pp. 307-312 ◽  
Author(s):  
J. VAN DE WALLE ◽  
R. J. TARENTO ◽  
P. JOYES
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Liquid Alloy ◽  
High Resolution Mass Spectrometry ◽  
Ion Source ◽  
High Resolution Mass ◽  
Isotopic Anomalies ◽  
Resolution Mass

A study of a Au–U liquid alloy ion source (LAIS) where uranium is enriched in its lighter element (20% 235U, 80% 238U) has been performed by high resolution mass spectrometry. Monoatomic species Au+, Au2+, U2+, U3+ and polyatomic species [Formula: see text], [Formula: see text], Au n U + are observed. The monoatomic results are well explained by the Kingham postionization process. [Formula: see text] and Au n U + species present an interesting odd–even effect characteristic of monovalent elements. The main result of this preliminary letter is a heteroisotope anomaly which appears in [Formula: see text], [Formula: see text] and [Formula: see text] emission where only homoisotopes are detected. A similar phenomenon was reported for [Formula: see text] and [Formula: see text] in a previous work and was attributed to a higher state of excitation of emitted heteroisotope aggregates which explode as they fly to the collector.1,2

scholarly journals Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS)

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Wanjin Sun ◽  
Junjie Zhang ◽  
Conghui Zhou ◽  
Bin Yan ◽  
Quan Cai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Principal Components ◽  
Collision Energy ◽  
High Resolution Mass Spectrometry ◽  
Ion Source ◽  
Negative Ion ◽  
Model Group ◽  
High Resolution Mass ◽  
Resolution Mass

Objective. To compare the differences in the serum principal components in ulcerative colitis- (UC-) induced rats, treated with compound Sophora decoction, matrine, oxymatrine monomer mixture, and indirubin monomer, and to provide a modern scientific basis for elucidating the clinical efficacy of compound Sophora decoction for the treatment of UC. Methods. The serum samples of rats from each group were obtained after drug administration, and the serum principal components of each group were analyzed by high-resolution mass spectrometry. Agilent Eclipse XDB C18 chromatographic column (100 mm × 2.1 mm, 3.5 m) was used for separation. The mobile phase was water (A) and methanol (B) (0.1% formic acid) gradient elution, 0–3 min (B: 20%–40%), 3–10 min (B: 40%–54%), 10–25 min (B: 54%), 25–35 min (B: 54%–70%), 35–45 min (B: 70%–80%), 45–50 min (B: 80%), 50–60 min (B: 80%–100%), 70–72 min (B: 100%–20%), and 72–77 min (B: 20%); flow rate, 300 μL/min; column temperature, 40°C; and injection volume, 10 μL. ESI source was selected and scanned in the positive and negative ion modes. The scanning range was 70–1500 m/z; ion-source gas 1 (GS1): 55 psi; ion-source gas 2 (GS2): 60 psi; CUR: 30 psi; ion-source temperature (TEM): 550°C; ion-source voltage (ISVF) : 5500 V/−4500 V; decluster voltage (DP): 100 V; collision energy (CE): 35 V/−35 V; collision energy gain (CES) : 15 V/−15 V; and data acquisition mode: IDA. After the data from each group were imported into MarkView 1.3, the molecular weights and retention times of different substances were obtained and qualitatively analyzed by ChemSpider and PeakView 2.0. Results. In the negative ion mode, 26 differential compounds were identified in the compound Sophora decoction group (FFKST) compared to the model group (M), and 18 differential compounds were identified in the matrine and oxymatrine group (KST) compared to the model group (M). In the positive ion mode, 11 and 7 differential compounds were identified in the compound Sophora decoction group (FFKST) and the matrine and oxymatrine group (KST) compared to the model group (M), respectively. The responses of all compounds in each group were compared with each other. As the different principal component substances in the indirubin group (DYH) displayed little correlation with other groups, the different components in this group were not researched thoroughly. Conclusion. By comparing the differences in the serum principal components from each administration group, we found that the FFKST group exhibited enhanced synthesis of the serum principal components; however, the compound doses of matrine and oxymatrine monomers did not exhibit the same changes in the serum principal components of UC-induced rats. Finally, the traditional Chinese medicine compound is more advantageous than monomers.

Analysis of residual gas by high-resolution mass spectrometry during helium glow discharge cleaning in JT-60U

2009 ◽  
Vol 84 (2-6) ◽  
pp. 908-910 ◽  
Author(s):  
Takao Hayashi ◽  
Atsushi Kaminaga ◽  
Takashi Arai ◽  
Masayasu Sato
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Glow Discharge ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Residual Gas ◽  
Resolution Mass

Exploring the Active Compounds of Traditional Mongolian Medicine Agsirga in Intervention of Novel Coronavirus (2019-nCoV) Based on HPLC-Q-Exactive-MS/MS and Molecular Docking Method

2020 ◽  
Author(s):  
Jie Cheng ◽  
Yuchen Tang ◽  
Baoquan Bao ◽  
Ping Zhang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
High Resolution ◽  
Mass Spectra ◽  
High Resolution Mass Spectrometry ◽  
Structural Formula ◽  
Active Compounds ◽  
High Resolution Mass ◽  
Q Exactive ◽  
Resolution Mass

<p><a></a><a></a><a></a><a><b>Objective</b></a>: To screen all compounds of Agsirga based on the HPLC-Q-Exactive high-resolution mass spectrometry and find potential inhibitors that can respond to 2019-nCoV from active compounds of Agsirga by molecular docking technology.</p> <p><b>Methods</b>: HPLC-Q-Exactive high-resolution mass spectrometry was adopted to identify the complex components of Mongolian medicine Agsirga, and separated by the high-resolution mass spectrometry Q-Exactive detector. Then the Orbitrap detector was used in tandem high-resolution mass spectrometry, and the related molecular and structural formula were found by using the chemsipider database and related literature, combined with precise molecular formulas (errors ≤ 5 × 10<sup>−6</sup>) , retention time, primary mass spectra, and secondary mass spectra information, The fragmentation regularities of mass spectra of these compounds were deduced. Taking ACE2 as the receptor and deduced compounds as the ligand, all of them were pretreated by discover studio, autodock and Chem3D. The molecular docking between the active ingredients and the target protein was studied by using AutoDock molecular docking software. The interaction between ligand and receptor is applied to provide a choice for screening anti-2019-nCoV drugs.</p> <p><b>Result</b>: Based on the fragmentation patterns of the reference compounds and consulting literature, a total of 96 major alkaloids and stilbenes were screened and identified in Agsirga by the HPLC-Q-Exactive-MS/MS method. Combining with molecular docking, a conclusion was got that there are potential active substances in Mongolian medicine Agsirga which can block the binding of ACE2 and 2019-nCoV at the molecular level.</p>

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

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