Role of protein kinase C in the regulation of electrical and contractile activity of smooth muscle: Effect of phorbol ester

1987 ◽  
Vol 104 (1) ◽  
pp. 879-882
Author(s):  
M. B. Baskakov ◽  
V. B. Studnitskii ◽  
M. A. Medvedev ◽  
B. I. Khodorov
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Phorbol Ester ◽  
Contractile Activity ◽  
Kinase C

Protein kinase C in cyclic stretch-induced nerve growth factor production by urinary tract smooth muscle cells

1995 ◽  
Vol 269 (4) ◽  
pp. C1018-C1024 ◽  
Author(s):  
K. Persson ◽  
J. J. Sando ◽  
J. B. Tuttle ◽  
W. D. Steers
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Urinary Tract ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Phorbol Ester ◽  
Cyclic Stretch ◽  
Bladder Obstruction ◽  
Kinase C

Cyclic stretch of cultured urinary tract smooth muscle cells has been used to mimic some of the events that occur with bladder obstruction. The stretch stimulus induces production of nerve growth factor (NGF), which has been implicated in changes in bladder innervation. Stretch-induced NGF production was blocked by actinomycin. Involvement of protein kinase C (PKC) in the stretch-induced NGF production is strongly suggested by the following observations. Phorbol ester activators of PKC mimicked the stretch response as did platelet-derived growth factor (PDGF), which acts, in part, through generation of endogenous diacylglycerols. Both stretch- and PDGF-induced NGF production were blocked by prolonged incubation with phorbol ester to downregulate PKC. Western blot analysis confirmed partial downregulation of the Ca(2+)-dependent PKC-alpha and PKC-beta 1 and near complete downregulation of the Ca(2+)-independent PKC isozymes delta, epsilon, and zeta. The involvement of PKC in transducing a physical stimulus (stretch) into a biochemical response (NGF production) has implications for novel types of therapeutic intervention in ailments such as bladder obstruction.

Mechanisms of smooth muscle contraction

1996 ◽  
Vol 76 (4) ◽  
pp. 967-1003 ◽  
Author(s):  
A. Horowitz ◽  
C. B. Menice ◽  
R. Laporte ◽  
K. G. Morgan
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Striated Muscle ◽  
Muscle Tone ◽  
Contractile Activity ◽  
Covalent Modification ◽  
Smooth Muscle Tissue ◽  
Initial Development ◽  
Kinase C

Work performed with differentiated contractile smooth muscle tissue over the last two decades has made clear that covalent modification of myosin by phosphorylation of the 20-kDa myosin light chains is a significant mode of regulation of contractile activity in smooth muscle, particularly in regard to the generation of phasic contractions and the initial development of tonic contractions. This regulatory mechanism appears to be of unique importance in smooth muscle compared with striated muscle. It is equally clear, however, that there is an important role for protein kinase C in the regulation of smooth muscle tone maintenance, particularly in vascular smooth muscle. Several possible signal transduction cascades involving protein kinase C are outlined. Increasing evidence suggests a link between protein kinase C and actin-based regulatory mechanisms. This review places emphasis on relating up-to-date biochemical facts to the physiological realities of the smooth muscle cell.

Possible role of Na+ influx in phorbol ester-induced down-regulation of protein kinase C in HL60 cells

FEBS Letters ◽  
1993 ◽  
Vol 328 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Noriko Takeuchi ◽  
Eikichi Hashimoto ◽  
Toru Nakamura ◽  
Fumito Takeuchi ◽  
Kiyonao Sada ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phorbol Ester ◽  
Down Regulation ◽  
Hl60 Cells ◽  
Kinase C
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