Mouse tooth germs in organ culture: Effects of ascorbate deficiency on histology and collagen synthesis

1978 ◽  
Vol 4 (4) ◽  
pp. 925-927
Author(s):  
Gordon E. Levenson
Keyword(s):  
Organ Culture ◽  
Collagen Synthesis ◽  
Tooth Germs ◽  
Culture Effects

Avian medullary bone in organ culture: Effects of vitamin D metabolites on collagen synthesis

1986 ◽  
Vol 39 (1) ◽  
pp. 35-43 ◽  
Author(s):  
John R. Harrison ◽  
Nancy B. Clark
Keyword(s):  
Vitamin D ◽  
Organ Culture ◽  
Collagen Synthesis ◽  
Vitamin D Metabolites ◽  
Medullary Bone ◽  
Culture Effects

Activation of serum complement inhibits collagen synthesis in fetal rat bone in organ culture

1982 ◽  
Vol 34 (1) ◽  
pp. 370-375 ◽  
Author(s):  
Barbara E. Kream ◽  
Lawrence G. Raisz ◽  
Ann L. Sandberg
Keyword(s):  
Organ Culture ◽  
Collagen Synthesis ◽  
Serum Complement ◽  
Fetal Rat ◽  
Rat Bone

Synthesis of taurocholate by rat fetal liver in organ culture: effects of cortisol in vitro.

1979 ◽  
Vol 237 (2) ◽  
pp. E177 ◽  
Author(s):  
T O Graham ◽  
D H Van Thiel ◽  
J M Little ◽  
R Lester
Keyword(s):  
Organ Culture ◽  
Dose Response ◽  
Incubation Medium ◽  
Response Pattern ◽  
Fetal Liver ◽  
Dry Weight ◽  
Organ Cultures ◽  
In Vitro Incubation ◽  
Culture Effects

Taurocholate production by fetal hepatic organ cultures was measured by radioimmunoassay. Taurocholate production was maximal on day 1 of in vitro incubation, but was demonstrable in organ cultures maintained for periods up to 15 days. Explants obtained from fetuses of 18 gestational days of age produced only 82 pmol taurocholate per milligram dry weight of tissue during the first 24 h of incubation. Explants obtained from fetuses 21 gestational days of age produced 1,043 pmol taurocholate per milligram dry weight. The presence of cortisol (2.0 X 10(-6) M) in the incubation medium increased synthesis of taurocholate by rat fetal liver in which total taurocholate rose 50-fold above control after 120 h of incubation. In increasing concentrations from 2.0 X 10(-9) M to 2.0 X 10(-7) M, cortisol produced an incremental rise in taurocholate. However, additional increases in cortisol dose failed to provide further stimulation, and taurocholate production was inhibited by cortisol concentrations of 2.0 X 10(-5) M. The results provide further validation for the technique of fetal hepatic organ culture. They demonstrate that taurocholate synthesis is increasing rapidly during the final stages of gestation and show that cortisol augments taurocholate synthesis in a dose-response pattern.

scholarly journals Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ

2020 ◽  
Vol 64 (1) ◽  
Author(s):  
Angammana Randilini ◽  
Kaoru Fujikawa ◽  
Shunichi Shibata
Keyword(s):  
Organ Culture ◽  
Developmental Stages ◽  
Tooth Development ◽  
Expression Patterns ◽  
Tooth Germ ◽  
Molar Tooth ◽  
Cervical Region ◽  
Metabolic Labeling ◽  
Tooth Formation ◽  
Tooth Germs

The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation. In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage, decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ. Biglycan mRNA was then expressed in the condensing mesenchyme and the dental papilla of the tooth germ. At the apposition stage (late bell stage), both decorin and biglycan mRNA were expressed in odontoblasts, resulting in a switch of the pattern of expression within the different stages of odontoblast differentiation. Decorin mRNA was expressed earlier in newly differentiating odontoblasts than biglycan. With odontoblast maturation and dentin formation, decorin mRNA expression was diminished and localized to the newly differentiating odontoblasts at the cervical region. Simultaneously, biglycan mRNA took over and extended its expression throughout the new and mature odontoblasts. Both mRNAs were expressed in the dental pulp underlying the respective odontoblasts. At P7.0, both mRNAs were weakly expressed but maintained their spatial expression patterns. Immunostaining showed that biglycan was localized in the dental papillae and pulp. In addition, all four SLRPs showed clear immunostaining in predentin, although the expressions of fibromodulin and lumican mRNAs were not identified in the tooth germs examined. The organ culture data obtained supported the histological findings that biglycan is more predominant than decorin at the apposition stage. These results were used to identify biglycan as the principal molecule among the SLRPs investigated. Our findings indicate that decorin and biglycan show spatial and temporal differential expressions and play their own tissue-specific roles in tooth development.

A Comparison of Collagen Synthesis by Different Categories of Human Chondrosarcoma in Organ Culture

1982 ◽  
Vol &NA; (168) ◽  
pp. 252???257
Author(s):  
DOUGLAS R. MILLER ◽  
HENRY J. MANKIN
Keyword(s):  
Organ Culture ◽  
Collagen Synthesis ◽  
Human Chondrosarcoma

Embryonic rat adrenal glands in organ culture: Effects of dexamethasone, nerve growth factor and its antibodies on pheochromoblast differentiation

1985 ◽  
Vol 241 (1) ◽  
pp. 207-217 ◽  
Author(s):  
Klaus Unsicker ◽  
ThomasJ. Millar ◽  
ThomasH. M�ller ◽  
Hans-D. Hofmann
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Organ Culture ◽  
Adrenal Glands ◽  
Nerve Growth ◽  
Culture Effects

Ultrastructure of in-vitro recovery of mineralization capacity of fluorotic enamel matrix in hamster tooth germs pre-exposed to fluoride in organ culture during the secretory phase of amelogenesis

1987 ◽  
Vol 32 (2) ◽  
pp. 107-115 ◽  
Author(s):  
D.M. Lyaruu ◽  
M. de Jong ◽  
A.L.J.J. Bronckers ◽  
J.H.M. Wöltgens
Keyword(s):  
Organ Culture ◽  
Enamel Matrix ◽  
Secretory Phase ◽  
Tooth Germs
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