Ethanol and sugar tolerance of Candida shehatae

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

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THE SUGAR TOLERANCE OF YEASTS EXPRESSED IN ATMOSPHERES

Science ◽

10.1126/science.74.1924.492 ◽

1931 ◽

Vol 74 (1924) ◽

pp. 492-493

Author(s):

M. B. Church

Keyword(s):

Sugar Tolerance

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Biodiversity of yeasts from Illinois maize

Canadian Journal of Microbiology ◽

10.1139/m97-050 ◽

1997 ◽

Vol 43 (4) ◽

pp. 362-367 ◽

Cited By ~ 15

Author(s):

M. J. R. Nout ◽

C. E. Platis ◽

D. T. Wicklow

Keyword(s):

Filamentous Fungi ◽

Debaryomyces Hansenii ◽

Candida Guilliermondii ◽

Illinois River ◽

Trichosporon Cutaneum ◽

Cryptococcus Albidus ◽

Candida Shehatae ◽

Pichia Membranifaciens ◽

Killer Yeasts ◽

Candida Zeylanoides

Microflora in wound sites of preharvest maize (including bacteria, yeasts, and filamentous fungi) may play a role in attracting insects to maize plants and may also interact with growth and mycotoxin production by filamentous fungi. As little data are available about the yeasts occurring on maize from the U.S. corn belt, samples of milled maize from experimental plantings at the University of Illinois River Valley Sand Field were analyzed. Yeast counts showed slight yearly fluctuation and varied between 3.60 and 5.88 (log cfu/g maize). The majority of the yeasts were Candida guilliermondii (approximately 55%), Candida zeylanoides (24 %), Candida shehatae (11%), and Debaryomyces hansenii (3%). Also present were Trichosporon cutaneum, Cryptococcus albidus var. aerius, and Pichia membranifaciens. The occurrence of killer yeasts was also evaluated. Killer yeasts were detected in maize for the first time and were identified as Trichosporon cutaneum and Candida zeylanoides. These were able to kill some representative yeasts isolated from maize, including Candida guilliermondii, Candida shehatae, and Cryptococcus albidus var. aerius. Other maize yeasts (Candida zeylanoides, Debaryomyces hansenii, Pichia membranifaciens) were not affected. The majority of yeasts found on maize were unable to ferment its major sugars, i.e., sucrose and maltose. Some (e.g., Candida zeylanoides) were not even able to assimilate these sugars. The importance of these properties in relation to insect attraction to preharvest ears of maize is discussed.Key words: corn, maize, yeast, killer.

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Transcriptomic analysis and driver mutant prioritization for differentially expressed genes from a Saccharomyces cerevisiae strain with high glucose tolerance generated by UV irradiation

RSC Advances ◽

10.1039/c7ra06146c ◽

2017 ◽

Vol 7 (62) ◽

pp. 38784-38797 ◽

Cited By ~ 1

Author(s):

Ying Chen ◽

Zhilong Lu ◽

Dong Chen ◽

Yutuo Wei ◽

Xiaoling Chen ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Glucose Tolerance ◽

Differentially Expressed Genes ◽

Uv Irradiation ◽

High Glucose ◽

Mutant Phenotype ◽

Differentially Expressed ◽

Driver Mutations ◽

High Sugar ◽

Sugar Tolerance

Driver mutations of a Saccharomyces cerevisiae mutant phenotype strain with high sugar tolerance were sought by the PheNetic network.

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The Ability of a Novel Strain Scheffersomyces (Syn. Candida) shehatae Isolated from Rotten Wood to Produce Arabitol

Polish Journal of Microbiology ◽

10.5604/01.3001.0010.4863 ◽

2017 ◽

Vol 66 (3) ◽

pp. 335-343 ◽

Cited By ~ 2

Author(s):

Monika Kordowska-Wiater ◽

Adam Kuzdraliński ◽

Tomasz Czernecki ◽

Zdzisław Targoński ◽

Magdalena Frąc ◽

...

Keyword(s):

Energy Density ◽

Plant Biomass ◽

Optimum Conditions ◽

Candida Shehatae ◽

Chemical Production ◽

Biotechnological Production ◽

Rotten Wood ◽

Optimization Study ◽

Pharmaceutical Industries ◽

Interesting Alternative

Arabitol is a polyalcohol which has about 70% of the sweetness of sucrose and an energy density of 0.2 kcal/g. Similarly to xylitol, it can be used in the food and pharmaceutical industries as a natural sweetener, a texturing agent, a dental caries reducer, and a humectant. Biotechnological production of arabitol from sugars represents an interesting alternative to chemical production. The yeast Scheffersomyces shehatae strain 20BM-3 isolated from rotten wood was screened for its ability to produce arabitol from L-arabinose, glucose, and xylose. This isolate, cultured at 28°C and 150 rpm, secreted 4.03 ± 0.00 to 7.97 ± 0.67 g/l of arabitol from 17–30 g/l of L-arabinose assimilated from a medium containing 20–80 g/l of this pentose with yields of 0.24 ± 0.00 to 0.36 ± 0.02 g/g. An optimization study demonstrated that pH 4.0, 32°C, and a shaking frequency of 150 rpm were the optimum conditions for arabitol production by the investigated strain. Under these conditions, strain 20BM-3 produced 6.2 ± 0.17 g/l of arabitol from 17.5 g/l of arabinose after 4 days with a yield of 0.35 ± 0.01 g/g. This strain also produced arabitol from glucose, giving much lower yields, but did not produce it from xylose. The new strain can be successfully used for arabitol production from abundantly available sugars found in plant biomass.

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A simple method to monitor hepatic gluconeogenesis and triglyceride synthesis following oral sugar tolerance test in obese adolescents

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00047.2019 ◽

2019 ◽

Vol 317 (1) ◽

pp. R134-R142 ◽

Cited By ~ 2

Author(s):

Anne-Marie Carreau ◽

Eunsook S. Jin ◽

Yesenia Garcia-Reyes ◽

Haseeb Rahat ◽

Kristen J. Nadeau ◽

...

Keyword(s):

Metabolic Diseases ◽

De Novo ◽

Tca Cycle ◽

Tolerance Test ◽

Serum Glucose ◽

Pentose Phosphate ◽

Invasive Technique ◽

Simple Method ◽

Obese Adolescents ◽

Sugar Tolerance

Hepatic energy metabolism is a key element in many metabolic diseases. Hepatic anaplerosis provides carbons for gluconeogenesis (GNG) and triglyceride (TG) synthesis. We aimed to optimize a protocol that measures hepatic anaplerotic contribution for GNG, TG synthesis, and hepatic pentose phosphate pathway (PPP) activity using a single dose of oral [U−13C3]glycerol paired with an oral sugar tolerance test (OSTT) in a population with significant insulin resistance. The OSTT (75 g glucose + 25 g fructose) was administered to eight obese adolescents with polycystic ovarian syndrome (PCOS) followed by ingestion of [U-13C3]glycerol at t = 180 or t = 210 min. 13C-labeling patterns of serum glucose and TG-glycerol were determined by nuclear magnetic resonance. 13C enrichment in plasma TG-glycerol was detectable and stable from 240 to 390 min with the [U-13C3]glycerol drink at t = 180 min(3.65 ± 2.3 to 4.47 ± 1.4%; P > 0.4), but the enrichment was undetectable at 240 min with the glycerol drink at t = 210 min. The relative contribution from anaplerosis was determined at the end of the OSTT [18.5 ±3.4% ( t = 180 min) vs. 16.0 ± 3.5% ( t = 210 min); P = 0.27]. [U-13C3]glycerol was incorporated into GNG 390 min after the OSTT with an enrichment of 7.5–12.5%. Glucose derived from TCA cycle activity was 0.3–1%, and the PPP activity was 2.8–4.7%. In conclusion, it is possible to obtain relative measurements of hepatic anaplerotic contribution to both GNG and TG esterification following an OSTT in a highly insulin-resistant population using a minimally invasive technique. Tracer administration should be timed to allow enough de novo TG esterification and endogenous glucose release after the sugar drink.

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