Mixed fermentation of Aspergillus niger and Candida shehatae to produce bioethanol with ionic-liquid-pretreated bagasse

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

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In Situ Saccharification of Cellulose in Mild Ionic Liquid Using Sodium Alginate Immobilized Cellulase

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.361-363.339 ◽

2013 ◽

Vol 361-363 ◽

pp. 339-342 ◽

Cited By ~ 3

Author(s):

Juan Juan Fei ◽

Qiang Li ◽

Yuan Yuan Feng ◽

Geng Sheng Ji ◽

Xu Ding Gu ◽

...

Keyword(s):

Ionic Liquid ◽

Sodium Alginate ◽

Reducing Sugars ◽

Enzymatic Saccharification ◽

Reducing Sugar ◽

Candida Shehatae ◽

Ionic Liquid Treatment ◽

Immobilized Cellulase

The work is to select biocompatible ionic liquid (IL) toward in situ saccharification of cellulose and investigating the effect of enzymatic saccharification with sodium alginate immobilized cellulase. The [Mmi [DM was selected for the ionic liquid treatment improved the yield of reducing sugars and the hydrolyzates could be efficiently fermented to ethanol. The yield of reducing sugar is 89.54% for 48h. In the in situ saccharification process, the yield of sugars were 84.52% and 86.72% with immobilized cellulase and free cellulase saccharification for 48h. Then the hydrolyzates could be fermented to ethanol withCandida shehatae. The yield of ethanol was 0.42g/g glucose within 24h.

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Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments

Biotechnology for Biofuels ◽

10.1186/s13068-017-0700-9 ◽

2017 ◽

Vol 10 (1) ◽

Cited By ~ 12

Author(s):

Paul Daly ◽

Jolanda M. van Munster ◽

Martin J. Blythe ◽

Roger Ibbett ◽

Matt Kokolski ◽

...

Keyword(s):

Ionic Liquid ◽

Aspergillus Niger ◽

Wheat Straw ◽

Compositional Changes

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Cellulose triacetate synthesis via one-pot organocatalytic transesterification and delignification of pretreated bagasse

RSC Advances ◽

10.1039/c8ra03859g ◽

2018 ◽

Vol 8 (39) ◽

pp. 21768-21776 ◽

Cited By ~ 11

Author(s):

Shiori Suzuki ◽

Yoshiki Shibata ◽

Daisuke Hirose ◽

Takatsugu Endo ◽

Kazuaki Ninomiya ◽

...

Keyword(s):

Ionic Liquid ◽

Lignocellulosic Biomass ◽

Cellulose Triacetate ◽

Transesterification Reaction ◽

One Pot ◽

Pretreated Bagasse ◽

Mild Acid

Cellulose triacetate was synthesised by the transesterification reaction of mild acid-pretreated lignocellulosic biomass with a stable acetylating reagent in an ionic liquid, EmimOAc, which enabled the dissolution of lignocellulose as well as the organocatalytic reaction.

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Effect of pretreatment on the formation of 5-chloromethyl furfural derived from sugarcane bagasse

RSC Advances ◽

10.1039/c5ra20203e ◽

2016 ◽

Vol 6 (7) ◽

pp. 5240-5248 ◽

Cited By ~ 6

Author(s):

Joshua Howard ◽

Darryn W. Rackemann ◽

Zhanying Zhang ◽

Lalehvash Moghaddam ◽

John P. Bartley ◽

...

Keyword(s):

Ionic Liquid ◽

Sugarcane Bagasse ◽

Acidic Ionic Liquid ◽

Pretreated Bagasse

The highest CMF yield (81.9%) was achieved with acidic ionic liquid pretreated bagasse, which had a glucan content of 81.6%.

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Study of a High-Yield Cellulase System Created by Heavy-Ion Irradiation-Induced Mutagenesis of Aspergillus niger and Mixed Fermentation with Trichoderma reesei

PLoS ONE ◽

10.1371/journal.pone.0144233 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0144233 ◽

Cited By ~ 7

Author(s):

Shu-Yang Wang ◽

Bo-Ling Jiang ◽

Xiang Zhou ◽

Ji-Hong Chen ◽

Wen-Jian Li ◽

...

Keyword(s):

Aspergillus Niger ◽

Trichoderma Reesei ◽

Ion Irradiation ◽

Heavy Ion ◽

High Yield ◽

Mixed Fermentation ◽

Heavy Ion Irradiation ◽

Induced Mutagenesis

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Comprehensive utilization of Aspergillus niger mycelium waste from citric acid fermentation on the basis of steam explosion and ionic liquid pretreatment

10.21203/rs.2.18638/v1 ◽

2019 ◽

Author(s):

Qinyuan Ma ◽

Xiuzhen Gao ◽

Xinyu Bi ◽

Menglei Xia ◽

Qi Han ◽

...

Keyword(s):

Ionic Liquid ◽

Citric Acid ◽

Aspergillus Niger ◽

Steam Explosion ◽

Soluble Sugar ◽

Acid Fermentation ◽

Ionic Liquid Pretreatment ◽

Comprehensive Utilization ◽

Citric Acid Fermentation ◽

Fungal Chitosan

Abstract Background Aspergillus niger mycelium waste, a by-product from citric acid fermentation is considered a good raw material to produce fungal chitosan and glucosamine (GlcN). However, its successful application has always been a challenge.Results In this work, the effects of combined pretreatment involving steam explosion and ionic liquid (IL) on mycelium waste were investigated. Results show that pretreatment of mycelium waste at 2.5 MPa for 1 min improved the GlcN hydrochloride yield by 65.49%, the release of soluble sugar reached 0.46 g/g of sample, and the deacelation degree of fungal chitosan by hydrolysis of chitin deacetylase from Rhodococcus equi CGMCC14861 (ReCDA) was from scratch and a 1.3-fold increase by combined with IL pretreatment. In addition, a recycling strategy for by-products, which promoted the fermentation of ReCDA used for fungal chitosan, was efficiently developed.Conclusion This is the first report of comprehensive utilization of Aspergillus niger mycelium waste from citric acid fermentation on the basis of steam explosion and ionic liquid pretreatment. This study demonstrates a green-like technology for the comprehensive utilization of A. niger mycelium waste from citric acid fermentation.

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