Changes in sugar beet leaf plasma membrane Fe(III)-chelate reductase activities mediated by Fe-deficiency, assay buffer composition, anaerobiosis and the presence of flavins

PROTOPLASMA ◽  
1998 ◽  
Vol 205 (1-4) ◽  
pp. 163-168 ◽  
Author(s):  
E. B. Gonz�lez-Vallejo ◽  
S. Sus�n ◽  
A. Abad�a ◽  
J. Abad�a
Keyword(s):  
Plasma Membrane ◽  
Sugar Beet ◽  
Fe Deficiency ◽  
Buffer Composition

Reduction of ferric chelates by leaf plasma membrane preparations from Fe-deficient and Fe-sufficient sugar beet

1999 ◽  
Vol 26 (6) ◽  
pp. 601 ◽  
Author(s):  
Elena B. González-Vallejo ◽  
Anunciación Abadía ◽  
Jose Antonio González-Reyes ◽  
Javier Abadía ◽  
Ana Flor López-Millán ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Citric Acid ◽  
Sugar Beet ◽  
Plasma Membranes ◽  
Ferric Iron ◽  
Reductase Activity ◽  
Tetraacetic Acid ◽  
Ferric Chelate Reductase ◽  
Ferric Chelate Reductase Activity ◽  
Optimal Ph

The ferric chelate reductase activities of leaf plasma membranes isolated from the leaves of Fe-deficient and Fe-sufficient sugar beet have been characterized. Substrates used were the complexes of ferric iron with ethylene diamine tetraacetic acid, citric acid and malic acid. Iron deficiency was associated with 1.5- to 2-fold increases in leaf plasma membrane ferric chelate reductase activity when rates were calculated on a protein basis. The natural complexes of ferric iron with citrate and especially with malate were good substrates for the ferric chelate reductase enzyme present in leaf plasma membrane preparations. The apparent affinities were higher for the ferric malate complex. The optimal pH for the activity of the ferric chelate reductase in sugar beet leaf plasma membranes was in the range 6.5–7.0. The ferric chelate reductase activity decreased by approximately 30% when the assay pH was decreased to 5.8 or increased to 7.5. Therefore, our data provide evidence against the hypothesis that changes in apoplastic pH could decrease markedly the activity of the ferric chelate reductase enzyme in plasma membrane preparations from the leaves of Fe-deficient plants.

Changes induced by Fe deficiency and Fe resupply in the organic acid metabolism of sugar beet (Beta vulgaris ) leaves

2001 ◽  
Vol 112 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Ana Flor López-Millán ◽  
Fermín Morales ◽  
Anunciación Abadía ◽  
Javier Abadía
Keyword(s):  
Sugar Beet ◽  
Organic Acid ◽  
Beta Vulgaris ◽  
Acid Metabolism ◽  
Fe Deficiency ◽  
Organic Acid Metabolism

Effects of temperature and buffer composition on calcium sequestration by sarcoplasmic reticulum and plasma membrane of rabbit renal artery

1990 ◽  
Vol 228 (3) ◽  
pp. 288-296
Author(s):  
Linda J. McGuffee ◽  
Sally A. Little ◽  
Juanita V. Mercure ◽  
Betty J. Skipper ◽  
Ellyn S. Wheeler-clark
Keyword(s):  
Plasma Membrane ◽  
Sarcoplasmic Reticulum ◽  
Renal Artery ◽  
Calcium Sequestration ◽  
Buffer Composition ◽  
Effects Of Temperature

scholarly journals OsYSL13 Is Involved in Iron Distribution in Rice

2018 ◽  
Vol 19 (11) ◽  
pp. 3537 ◽  
Author(s):  
Chang Zhang ◽  
Kamran Shinwari ◽  
Le Luo ◽  
Luqing Zheng
Keyword(s):  
Plasma Membrane ◽  
Plant Growth ◽  
Human Health ◽  
Brown Rice ◽  
Expression Level ◽  
Fe Deficiency ◽  
Wild Type ◽  
Iron Distribution ◽  
Yellow Stripe ◽  
Uptake And Transport

The uptake and transport of iron (Fe) in plants are both important for plant growth and human health. However, little is known about the mechanism of Fe transport in plants, especially for crops. In the present study, the function of yellow stripe-like 13 (YSL13) in rice was analyzed. OsYSL13 was highly expressed in leaves, especially in leaf blades, whereas its expression was induced by Fe deficiency both in roots and shoots. Furthermore, the expression level of OsYSL13 was higher in older leaves than that in younger leaves. OsYSL13 was located in the plasma membrane. Metal measurement revealed that Fe concentrations were lower in the youngest leaf and higher in the older leaves of the osysl13 mutant under both Fe sufficiency and deficiency conditions, compared with the wild type and two complementation lines. Moreover, the Fe concentrations in the brown rice and seeds of the osysl13 mutant were also reduced. Opposite results were found in OsYSL13 overexpression lines. These results suggest that OsYSL13 is involved in Fe distribution in rice.

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