Role of magnesium in the (Ca2++Mg2+)-stimulated membrane ATPase of human red blood cells

1977 ◽  
Vol 35 (1) ◽  
pp. 149-158 ◽  
Author(s):  
H. J. Schatzmann
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Membrane Atpase

scholarly journals Detection of acyl-CoA-binding protein in human red blood cells and investigation of its role in membrane phospholipid renewal

1995 ◽  
Vol 306 (3) ◽  
pp. 793-799 ◽  
Author(s):  
H Fyrst ◽  
J Knudsen ◽  
M A Schott ◽  
B H Lubin ◽  
F A Kuypers
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Liver ◽  
Plasma Membranes ◽  
Binding Protein ◽  
Human Red Blood Cells ◽  
Low Concentrations ◽  
Membrane Bound ◽  
High Concentrations

Acyl-CoA-binding protein (ACBP) has been identified in a number of tissues and shown to affect the intracellular distribution and utilization of acyl-CoA. We have detected ACBP in the cytosol but not the membrane of human red blood cells and, using an e.l.i.s.a. with antibodies prepared against human liver ACBP, found that its concentration was 0.5 microM. To investigate the role of ACBP in human red blood cells, we added purified human liver ACBP and radiolabelled acyl-CoA to isolated membranes from these cells. ACBP prevented high concentrations of acyl-CoA from binding to the membrane but could not keep the acyl-CoA in the aqueous phase at low concentrations. This suggested the presence of a pool in the membrane with a binding affinity for acyl-CoA that was greater than that of ACBP for acyl-CoA. In the presence of lysophospholipid, this membrane-bound pool of acyl-CoA was rapidly used as a substrate by acyl-CoA:lysophospholipid acyltransferase (LAT) to generate phospholipid from lysophospholipid. We also found that ACBP-bound acyl-CoA was preferred over free acyl-CoA as a substrate by LAT. These results are the first documentation that human red blood cells contain ACBP and that this protein can affect the utilization of acyl-CoA in plasma membranes of these cells. The interactions between acyl-CoA, ACBP and the membrane suggest that there are several pools of acyl-CoA in the human red blood cell and that ACBP may have a role in regulating their distribution and fate.

scholarly journals Investigations on the role of hemoglobin in sulfide metabolism by intact human red blood cells

2018 ◽  
Vol 149 ◽  
pp. 163-173 ◽  
Author(s):  
Christopher L. Bianco ◽  
Anton Savitsky ◽  
Martin Feelisch ◽  
Miriam M. Cortese-Krott
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

scholarly journals How do red blood cells know when to die?

2017 ◽  
Vol 4 (4) ◽  
pp. 160850 ◽  
Author(s):  
Clemente Fernandez Arias ◽  
Cristina Fernandez Arias
Keyword(s):  
Theoretical Model ◽  
Red Blood Cells ◽  
Conceptual Model ◽  
Blood Cells ◽  
Phagocytic Activity ◽  
Oxygen Availability ◽  
Unified Framework ◽  
Human Red Blood Cells

Human red blood cells (RBCs) are normally phagocytized by macrophages of splenic and hepatic sinusoids at 120 days of age. The destruction of RBCs is ultimately controlled by antagonist effects of phosphatidylserine (PS) and CD47 on the phagocytic activity of macrophages. In this work, we introduce a conceptual model that explains RBC lifespan as a consequence of the dynamics of these molecules. Specifically, we suggest that PS and CD47 define a molecular algorithm that sets the timing of RBC phagocytosis. We show that significant changes in RBC lifespan described in the literature can be explained as alternative outcomes of this algorithm when it is executed in different conditions of oxygen availability. The theoretical model introduced here provides a unified framework to understand a variety of empirical observations regarding RBC biology. It also highlights the role of RBC lifespan as a key element of RBC homeostasis.

Role of intracellular Ca2+ on the formation of microvesicle in human red blood cells

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Nguyen Duc Bach ◽  
Nguyen Huu Duc ◽  
Pham Kim Dang ◽  
Luu Thao Linh ◽  
Ly Thi Bich Thuy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

scholarly journals The Immunologic Specificity of Antiserum for Trypsin-Treated Red Blood Cells and Its Reactions with Normal and Hemolytic Anemia Cells

Blood ◽  
1953 ◽  
Vol 8 (7) ◽  
pp. 640-647 ◽  
Author(s):  
MATTHEW C. DODD ◽  
CLAUDE-STARR WRIGHT ◽  
J. ALBERT BAXTER ◽  
BERTHA A. BOURONCLE ◽  
ALVINZA E. BUNNER ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Anemia ◽  
Blood Cells ◽  
Absorption Data ◽  
Normal Cells ◽  
Human Red Blood Cells ◽  
Dual Specificity ◽  
Absorption Studies ◽  
Specific Fraction

Abstract 1. Rabbits were immunized with both normal (Nrbc) and trypsinized (Trbc) human red blood cells and the antisera examined with normal, trypsinized, periodate-treated, and hemolytic anemia cells. 2. Absorption studies showed the presence of a fraction in both anti-Trbc and anti-Nrbc that was specific for trypsinized cells. 3. This T cell specific fraction from anti-Trbc serum (anti-TE) did not agglutinate or sensitize normal red blood cells, but agglutinated periodate-treated cells. This latter specificity was shown to be a part of the modification produced by trypsinization. 4. Anti-TE also agglutinated the cells of fifteen of nineteen patients with acquired hemolytic anemia and three of thirteen cases of hereditary spherocytosis. 5. Antibody for trypsinized and normal cells was also detected in antiserum to normal cells. Absorption data suggested the presence in this antiserum of antibody with a dual specificity for both types of cells. 6. The role of the antigenic modifications produced by trypsin in red cell immunization and in hemolytic anemia is discussed.

Role of chloride in potassium transport through a K-Cl cotransport system in human red blood cells

1989 ◽  
Vol 256 (5) ◽  
pp. C994-C1003 ◽  
Author(s):  
C. Brugnara ◽  
T. Van Ha ◽  
D. C. Tosteson
Keyword(s):  
Membrane Potential ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Normal Subjects ◽  
Similar Data ◽  
Human Red Blood Cells ◽  
Dense Fraction ◽  
Cotransport System ◽  
Low K

In this paper, we report experiments demonstrating the coupling of Cl and K movements in a volume-dependent K-Cl cotransport system in human red blood cells. We show that an outwardly directed Cl gradient can promote net K efflux against an inwardly directed K gradient at constant membrane potential. Red blood cell membrane potential was kept constant by using anions that are not transported through the K-Cl cotransport system but that are more permeable than Cl (NO3 and SCN). Under these conditions, when the activities of band 3 (capnophorin)-mediated anion exchange and of the carbonic anhydrase have been inhibited, it is possible to maintain a Cl gradient at constant membrane potential. Similar data were obtained in human red blood cells (least dense fraction from normal subjects and whole blood from patients with homozygous hemoglobin S disease), in rabbit red blood cells, and in low-K sheep red blood cells. These data confirm that the volume-dependent Cl-dependent K movement in these cells operates through coupled K-Cl cotransport.

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