Hemolytic action of staphylococcal α-hemolysin on human erythrocytes in a Na+- and K+-containing suspending fluid

1970 ◽  
Vol 36 (1) ◽  
pp. 57-65 ◽  
Author(s):  
R. C. A. Sengers
1980 ◽  
Vol 18 (4) ◽  
pp. 586-592 ◽  
Author(s):  
J Brajtburg ◽  
G Medoff ◽  
G S Kobayashi ◽  
S Elberg ◽  
C Finegold

1959 ◽  
Vol 110 (1) ◽  
pp. 93-102 ◽  
Author(s):  
David W. Soule ◽  
Guido V. Marinetti ◽  
Herbert R. Morgan

Hemolysis of chicken red blood cells by mumps virus is associated with the release of sphingomyelin from the stromal lipoprotein and the destruction of 65 per cent of the sphingomyelin of the red cell stroma. However, the virus had no effect on isolated phosphatides extracted from the erythrocytes. The hemolytic action of the virus and changes in sphingomyelin content of the erythrocytes fail to occur at a pH of 6.0. The viral hemolysis of human erythrocytes is not associated with similar alterations in their content of sphingomyelin. The absence of lecithin from sheep erythrocytes, which are also lysed by mumps virus, is additional evidence that a viral lecithinase is not associated with the hemolytic property of mumps virus. Mumps virus concentrated from the amniotic fluid of viral infected chick embryos contains about 7 per cent phosphatide, 60 per cent of which is sphingomyelin.


Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.


1960 ◽  
Vol XXXIV (II) ◽  
pp. 305-311 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos

ABSTRACT The red cell triiodothyronine uptake technique as used in our hospital is described. Incubation time is of almost no importance. The temperature during incubation should be 37° C. Further improvement of the technique is obtained when all blood samples are brought up to 40 % haematocrit prior to incubation. Clinical results are discussed. It is yet too early to give a definite assessment of its clinical value, but it is definitely superior to the measurement of the BMR.


Author(s):  
Mohammed Ibrahim ◽  
Alaa Zaky ◽  
Mohsen Afouna ◽  
Ahmed Samy

Carrier erythrocytes are emerging as one of the most promising biological drug delivery systems investigated in recent decades. Beside its biocompatibility, biodegradability and ability to circulate throughout the body, it has the ability to perform extended release system of the drug for a long period. The ultimate goal of this study is to introduce a new carrier system for Salbutamol, maintaining suitable blood levels for a long time, as atrial to resolve the problems of nocturnal asthma medication Therefore in this work we study the effect of time, temperature as well as concentration on the loading of salbutamol in human erythrocytes to be used as systemic sustained release delivery system for this drug. After the loading process is performed the carrier erythrocytes were physically and cellulary characterized. Also, the in vitro release of salbutamol from carrier erythrocytes was studied over time interval. From the results it was found that, human erythrocytes have been successfully loaded with salbutamol using endocytosis method either at 25 Co or at 37 Co . The highest loaded amount was 3.5 mg/ml and 6.5 mg/ml respectively. Moreover, the percent of cells recovery is 90.7± 1.64%. Hematological parameters and osmotic fragility behavior of salbutamol loaded erythrocytes were similar that of native erythrocytes. Scanning electron microscopy demonstrated that the salbutamol loaded cells has moderate change in the morphology. Salbutamol releasing from carrier cell was 43% after 36 hours in phosphate buffer saline. The releasing pattern of the drug from loaded erythrocytes showed initial burst release in the first hour followed by a very slow release, obeying zero order kinetics. It concluded that salbutamol is successfully entrapped into erythrocytes with acceptable loading parameters and moderate morphological changes, this suggesting that erythrocytes can be used as prolonged release carrier for salbutamol.


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