Optimization of γ-linolenic acid (GLA) production inSpirulina platensis

1994 ◽  
Vol 6 (3) ◽  
pp. 295-300 ◽  
Author(s):  
M. Tanticharoen ◽  
M. Reungjitchachawali ◽  
B. Boonag ◽  
P. Vonktaveesuk ◽  
A. Vonshak ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Gla Production

Enhancing the production of gamma-linolenic acid in Hansenula polymorpha by fed-batch fermentation using response surface methodology

2011 ◽  
Vol 65 (2) ◽  
Author(s):  
Bhimabol Khongto ◽  
Kobkul Laoteng ◽  
Anan Tongta
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Linolenic Acid ◽  
Yeast Extract ◽  
Batch Fermentation ◽  
Hansenula Polymorpha ◽  
Gamma Linolenic Acid ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Gla Production

AbstractGamma-linolenic acid (GLA, C18:3Δ6,9,12) is an n-6 polyunsaturated fatty acid (PUFA) that has been used for the alleviation and treatment of a number of symptoms and diseases. Increasing GLA demand has led to a search for alternative producers and potential strategies for GLA production. Based on the successful performance of Hansenula polymorpha, a methylotrophic yeast, as a “cell factory” for the production of valuable bioproducts, a bioprocess development approach was implemented for GLA production in the recombinant yeast carrying the mutated Δ6-desaturase gene of Mucor rouxii. Using a substrate-feeding strategy under glycerol-limited conditions, the physical-chemical variables during the fed-batch fermentation of the recombinant H. polymorpha were optimised for GLA production through response surface methodology using a Box-Behnken design. The medium composition, including yeast extract and trace elements, and dissolved oxygen tension (DOT) were targeted. We found that DOT was the most effective variable for enhancing GLA yield. These results also suggest that the optimum conditions for GLA production are 28 % saturation of DOT, 1 g L−1 of yeast extract and 3.6 mL L−1 of the Pichia trace metals 1 (PTM1).

Media Optimization for the Production of ?- Linolenic Acid by Cunninghamella echinulata var.elegans MTCC 552 Using Response Surface Methodology

2008 ◽  
Vol 4 (2) ◽  
Author(s):  
Amit Shrivastava ◽  
Ishwar Bajaj ◽  
Parag Saudagar ◽  
Rekha Singhal
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Linolenic Acid ◽  
Fungal Species ◽  
Biologically Active ◽  
Mucor Rouxii ◽  
Primary Metabolite ◽  
Cunninghamella Echinulata ◽  
Effects Of Media ◽  
Gla Production

?- Linolenic acid (GLA) is an intracellular primary metabolite produced by some plants and fungal species via several distinct biosynthetic pathways. It is an essential fatty acid, and is mainly used in treatment of various diseases such as rheumatoid arthritis, multiple sclerosis, schizophrenia, atopic eczema and premenstrual syndrome. It is also a precursor of a number of biologically active compounds. In present work, strains of Cunninghamella echinulata var.elegans MTCC 552, Mucor rouxii MTCC 386, Mucor hiemallis MTCC 1278 and Rhizopus stolnifer MTCC 2591 were screened for maximum production of GLA. Cunninghamella echinulata var. elegans MTCC 552 produced maximum GLA (13.7 ± 0.31 mg/g of DCW), and hence selected for further work. Many crucial factors that affect GLA production were optimized in two steps. In the first step, one factor at a time method was used to investigate the effects of media constituents. Subsequently, in the second step, concentration of media components was optimized using Response Surface Methodology (RSM). These optimizations increased the GLA production to 19.8 ± 0.35 mg/g of DCW.

scholarly journals An Efficient Method for Extraction and Enrichment of γ-Linolenic Acid (GLA) from Spirulina

2021 ◽  
Vol 11 (1) ◽  
pp. 3166-3174
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Gas Chromatography ◽  
Linolenic Acid ◽  
The World ◽  
Dry Biomass ◽  
Urea Crystallization ◽  
One Step ◽  
Optimized Conditions ◽  
Gla Production

γ-linolenic acid (GLA) is useful for improving and treating various diseases, including immune system-related diseases. In the world of commerce, GLA production has problems that compel scientists to search for a simpler, safer, and less costly way. Some microalgae have been introduced as a rich source of fatty acids; Spirulina platensis has attracted scientists due to the presence of 31% GLA in its fatty acid profile. In the present study, the Iranian strain of spirulina was investigated. Cell culture was performed under optimized conditions and after dry biomass production; the powder was used to continue the study. Finally, the extraction and enrichment steps were performed, and the IR spectra and gas chromatography methods were used for identification and final measurements, respectively. After enrichment by one-step urea crystallization, the percentage of GLA enriched in the total lipid was increased to 61.17%. These results suggest that employing simple, relatively inexpensive, and modified methods for the GLA isolation from the Iranian strain of spirulina may have a potential for industrialization.

Dihomo-γ-Linolenic Acid in Patients with Atherosclerosis: Effects on Platelet Aggregation, Plasma Lipids and Low-Density Lipoprotein-Induced Inhibition of Prostacyclin Generation

1984 ◽  
Vol 51 (02) ◽  
pp. 186-188 ◽  
Author(s):  
A Szczeklik ◽  
R J Gryglewski ◽  
K Sladek ◽  
E Kostka-Trąbka ◽  
A Żmuda
Keyword(s):  
Linolenic Acid ◽  
Plasma Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Thromboxane A2 ◽  
Low Density ◽  
Red Cell ◽  
Double Blind ◽  
Daily Dose ◽  
Antithrombotic Effects

SummaryDihomo-γ-linolenic acid (DHLA), a precursor of monoenoic anti-aggregatory prostaglandins (PGE1, PGD2), was administered for 4 weeks in a daily dose of 1.0 g into 33 patients with atherosclerosis on a basis of a double-blind trial. Comparison of treatment and placebo groups revealed elevation of DHLA in red cell lipids in DHLA-treated subjects. No differences, however, between the two groups could be observed in platelet aggregability, thromboxane A2 generation by platelets, serum cholesterol, PGE1 and PGE2 levels, and in inhibitory activity of low-density lipoproteins against prostacyclin synthetizing system in arteries. The dietary supplementation used did not lead to distinct antithrombotic effects.

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