In the Far East of Russia the genus Serratula L. (family Asteraceae) is represented by 2 species - Serratula manshurica Kitag. and Serratula komarovii Iljin, which can be potential sources of biologically active compounds (phytoecdysteroids). For the first time in the aerial part (leaves, stems, inflorescences) of Serratula komarovii 6 flavonoid compounds (luteolin-7-O-β-D-glucuronopyranoside, apigenin-7-O-β-D-glucuronopyranoside, chrysoeriol-7-O-β-D-glucuronopyranoside, quercetin, luteolin, 3-methylquercetin) were identified by liquid extraction (70% ethyl alcohol) and reverse phase liquid chromatography (RP HPLC). The structure of compounds (luteolin-7-O-β-D-glucuronopyranoside, apigenin-7-O-β-D-glucuronopyranoside, chrysoeriol-7-O-β-D-glucuronopyranoside), isolated by preparative column chromatography on silica gel in a gradient elution with a mixture of solvents (carbon tetrachloride and ethyl alcohol) was proved by UV and NMR-1H, -13C, 1H-,13C-HMBC spectroscopy. It was noted that the dominant flavonoid in Serratula komarovii is luteolin-7-O-β-D-glucuronopyranoside (in the leaves – 4.92±0.98% and in the stems – 1.23±0.25%), and in inflorescences – apigenin-7-O-β-D-glucuronopyranoside (1.10±0.22%). The method of differential spectrophotometry was used to determine the sum of flavonoids in the aerial organs of Serratula komarovii which varies from 1.96 to 9.04%. The maximum content of flavonoids was detected in the leaves – 9.04±0.71%, and the minimum in the stems of the plant – 1.96±0.20%. Thus, Serratula komarovii can be a promising and constantly renewable source of not only phytoecdysteroids, but also flavonoids necessary for the pharmaceutical industry.