Effect of age on the conversion of thyroxine (T4) to 3,5,3′ triiodothyronine (T3) by liver homogenate from fed and fasted Sprague-Dawley rats

AGE ◽  
1982 ◽  
Vol 5 (3) ◽  
pp. 88-91 ◽  
Author(s):  
Steven R. Gambert
Keyword(s):  
Liver Homogenate ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Effect Of Age

Plasma Cocaine Metabolite Levels and Liver CYP450 3A4 Isoenzyme Activity as Indicators of Cocaine Metabolism in Rats Treated With Salako Supplements

2019 ◽  
pp. 1-21
Author(s):  
Natwaine Sherune Gardner ◽  
Kedon J. S. Luke ◽  
Andrew O. Wheatley ◽  
Winston De La Haye ◽  
Perceval Steven Bahado-Singh ◽  
...  
Keyword(s):  
Plasma Clearance ◽  
Liver Homogenate ◽  
Nutritional Supplements ◽  
Test Results ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Sprague Dawley Rats ◽  
Chronic Cocaine ◽  
One Way Anova ◽  
Range Test

The effects of Salako nutritional supplements on cocaine-dependent Sprague Dawley rats was investigated. Rats were made cocaine-dependent using conditioned place preference (CPP) where craving was analyzed regularly. Cocaine metabolite levels were determined from blood samples. CYP450 3A4 isoenzyme activities were obtained using liver homogenate. Statistical analysis was done using SPSS one-way ANOVA and Duncans multiple range test. Results show that when cocaine use was discontinued, the supplements reduced craving of cocaine significantly. Blood plasma results showed higher benzoylecgonine equilibrium possibly indicating that the supplements aided the removal of stored cocaine metabolites which may have contributed to better management of craving in the rats. CYP450 3A4 isoenzyme activity was further enhanced by the supplements and is indicative of increased cocaine metabolism. The results indicate that the Salako nutritional supplements reduce craving caused by chronic cocaine administration by increasing the liver CYP450 3A4 isoenzyme activity, resulting in better plasma clearance.

Effect of Age on Protein Conservation during Very-Low-Energy Diet in Obese Sprague-Dawley Rats

1998 ◽  
Vol 6 (6) ◽  
pp. 448-457 ◽  
Author(s):  
Julia A. Johnson ◽  
Choon-Hie Yu ◽  
Mei-Uih Yang ◽  
F. Xavier Pi-Sunyer
Keyword(s):  
Low Energy ◽  
Sprague Dawley ◽  
Very Low Energy Diet ◽  
Sprague Dawley Rats ◽  
Protein Conservation ◽  
Effect Of Age ◽  
Low Energy Diet

The Effect of Age and Strain Differences on the Incidence of Restraint-Induced Oral and Gastric Ulcers in Three Strains of Rats

1972 ◽  
Vol 51 (2) ◽  
pp. 619-625 ◽  
Author(s):  
D.K. Whittaker ◽  
T.R. Wilson
Keyword(s):  
Wistar Rats ◽  
Strain Differences ◽  
Gastric Ulcers ◽  
Sprague Dawley ◽  
Oral Ulcers ◽  
Sprague Dawley Rats ◽  
Different Strains ◽  
Strain Dependent ◽  
Effect Of Age

Restraint of different strains of rat has been shown to be capable of inducing both oral and gastric ulcers. Hooded Wistar rats were most susceptible to gastric ulcers, whereas Sprague-Dawley rats had the highest incidence of oral ulcers. The causative mechanisms probably differ and are strain dependent.

scholarly journals Pengaruh faktor umur dan pemberian serbuk biji gandum terhadap jumlah osteoblas pada pergerakan gigi secara ortodontiThe effect of age and wheat seed powder application on the number of osteoblasts on orthodontic tooth movement

2020 ◽  
Vol 32 (3) ◽  
pp. 193
Author(s):  
Idayu Nafila ◽  
Putri Aulia Sari ◽  
Annisa Firdha ◽  
Sri Suparwitri
Keyword(s):  
Triticum Aestivum ◽  
Tooth Movement ◽  
Bone Remodelling ◽  
Orthodontic Tooth Movement ◽  
Coil Spring ◽  
Wheat Seed ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Maxillary Incisors ◽  
Effect Of Age

Pendahuluan: Pergerakan gigi pada perawatan ortodonti merupakan kombinasi proses resorpsi oleh osteoklas pada daerah tertekan dan aposisi oleh osteoblas pada sisi tertarik sehingga terjadi remodeling tulang. Remodeling tulang dipengaruhi umur dan hormon estrogen. Biji gandum (Triticum aestivum) merupakan fitoestrogen yang memiliki struktur dan fungsi seperti hormon estrogen. Tujuan penelitian menganalisis pengaruh faktor umur dan pemberian serbuk biji gandum terhadap jumlah osteoblas pada pergerakan gigi secara ortodonti pada tikus Sprague dawley muda dan tua. Metode: Jenis penelitian eksperimental laboratoris dengan menggunakan 36 subjek tikus Sprague dawley jantan yang terbagi menjadi kelompok muda (4-5 minggu) dan tua (18-20 minggu). Setiap grup akan dibagi menjadi 2 subgrup untuk menerima perlakuan yaitu: kelompok kontrol dengan perlakuan insisivus maksila digerakan ke distal menggunakan koil spring dan kelompok perlakuan yang diberi serbuk biji gandum dengan dosis 1,08 g dan perlakukan insisivus maksila digerakan ke distal menggunakan koil spring. Subjek hewan coba dieutanasia menggunakan anestesi dosis mematikan dan dilakukan pemotongan rahang atas pada regio kedua insisivus pada hari ke 1, 4, dan 7. Osteoblas dianalisis secara histologi dengan pengecatan hematoksilin eosin. Perhitungan jumlah osteoblas dilakukan dengan menggunakan mikroskop optilab. Hasil: Jumlah osteoblas kelompok muda lebih banyak dibandingkan kelompok tua. Pemberian serbuk biji gandum dapat meningkatkan jumlah osteoblas pada kelompok tua maupun muda. Pemberian serbuk biji gandum menunjukan pengaruh  signifikan p<0,001 (p<0,05) terhadap jumlah osteoblas Simpulan: Terdapat pengaruh faktor umur dan pemberian serbuk biji gandum terhadap peningkatan jumlah osteoblas pada pergerakan gigi secara ortodonti, osteoblas pada tikus berumur muda lebih banyak dibandingkan dengan tikus berumur tua.Kata kunci: Osteoblas, pergerakan gigi secara ortodonti, serbuk gandum, fitoestrogen, tikus Sprague dawley. ABSTRACTIntroduction: Tooth movement in orthodontic treatment is a combination of osteoclasts resorption in the stressed area and osteoblast apposition on the pulled side, resulting in bone remodelling. Bone remodelling is influenced by age and estrogen hormone. Wheat seeds (Triticum aestivum) are phytoestrogens with estrogen-like structure and function. This study was aimed to analyse the effect of age and wheat seed powder on the number of osteoblasts on orthodontic tooth movement in young and old Sprague-Dawley rats. Methods: This study was an experimental laboratory using 36 male Sprague-Dawley rats divided into young (4-5 weeks) and old (18-20 weeks) groups. Each group will be divided into two subgroups to receive treatment, namely: the control group with the treatment of the maxillary incisors moved distally using a coil spring and the treatment group treated with wheat seed powder at a dose of 1.08 g and treated the maxillary incisors were moved distally using a coil spring. Animal subjects were euthanised using lethal doses of anaesthesia, and the maxilla was cut in the second region of the incisors on the 1st, 4th, and 7th days. Osteoblasts were analysed histologically by eosin hematoxylin staining. The number of osteoblasts was calculated using an Optylab microscope. Results: The number of osteoblasts in the young group was found to be higher than the old group. The application of wheat seed powder can increase the number of osteoblasts in the young and old groups. The application of wheat seed powder showed a significant (p<0.001 (p<0.05)) on the number of osteoblasts. Conclusion: There was an effect of age and wheat seed powder on the increase in the number of osteoblasts on orthodontic tooth movement. The young rats have more osteoblasts compared to the old rats.Keywords: Osteoblasts, orthodontic tooth movement, wheat seed powder, phytoestrogen, Sprague-Dawley rats.

Effect of age, calcium source, and radiolabeling method on whole body 47Ca retention in the rat

1993 ◽  
Vol 265 (4) ◽  
pp. E554-E558 ◽  
Author(s):  
M. B. Andon ◽  
R. L. Kanerva ◽  
M. C. Schulte ◽  
K. T. Smith
Keyword(s):  
Repeated Measures ◽  
Rank Order ◽  
Whole Body ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Repeated Measures Analysis ◽  
Calcium Source ◽  
The Mean ◽  
Rate Of Decline ◽  
Effect Of Age

In a longitudinal study we determined the effect of animal age as well as Ca source and radiolabeling method on Ca bioavailability by measuring whole body 47Ca retention (WBR) in male Sprague-Dawley rats. The WBR assay was performed without surgery or anesthesia, and the same groups of animals were studied at 8, 16, 20, and 32 wk of age. Rats were administered a 6-mg radiolabeled oral dose of Ca as Ca citrate malate (CCM) or intrinsically or extrinsically labeled CaCO3 or hydroxyapatite (HAP). Fractional Ca retention was measured from the 72-h postdose WBR divided by WBR at time 0. WBR was significantly affected by Ca source with CCM > CaCO3 > HAP at all ages (P < 0.001). The rank order and relative bioavailabilities of these Ca salts in the rat model agreed well with literature values for Ca absorption in adult humans. Although percent WBR decreased significantly with advancing age (P < 0.001), the mean rate of decline (-3.4%/wk) was not affected by Ca source. Extrinsic radiolabeling overestimated (approximately 20%) Ca bioavailability when the rats were young. However, the magnitude of this effect diminished with advancing animal age and was not significant across all ages (repeated measures analysis of variance P = 0.10).

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

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