GC determination of nicotine in subcutaneous adipose tissue obtained by minimal trauma tissue biopsy

Background: Obesity is associated with increased rate of mortality and morbidity if untreated. Obesity is a health hazard it required attention towards adolescent and adult age group. Generally, body fat is distributed into two main compartments that are subcutaneous adipose tissue and visceral adipose tissue. Waist Hip Ratio measures the subcutaneous adipose tissue, determination of visceral adiposity will further helpful for determination of cardiovascular disease.Methods: The study comprised of 120 subjects, of these adolescents comprised of 60 healthy adolescent childrens. The adults comprised of 60 healthy individuals. In all the subjects, Height was estimated in centimetres and Weight was measured in kilogram measured and BMI was calculated. The waist hip ratio and visceral adiposity index was calculated. In all the subjects, venous blood was collected from all the subjects for lipid profile estimation.Results: In present study BMI more than 25 have soon increased waist hip ratio and visceral adiposity index value than BMI less than 25 and it’s statically significant in both adolescent and adult group. It is also reported that BMI more than 25 have increased level of mean serum triglycerides, serum total cholesterol, LDL cholesterol and VLDL the increase was statistically significant. The mean serum HDL cholesterol was significantly decreased in BMI>25 group when compared with BMI<25.Conclusions: It was concluded that different age groups of adolescent and adult, appropriate methods should be planned specifically for adolescent and adult age group. Regular educational sessions are needed to make changes adolescent and adults toward obesity.

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Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies

Methods in Molecular Biology - Amyloid Proteins ◽

10.1007/978-1-61779-551-0_24 ◽

2012 ◽

pp. 363-371 ◽

Cited By ~ 13

Author(s):

Per Westermark

Keyword(s):

Adipose Tissue ◽

Subcutaneous Adipose Tissue ◽

Amyloid Protein ◽

Tissue Biopsy ◽

Subcutaneous Adipose

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Subcutaneous adipose tissue exerts proinflammatory cytokines after minimal trauma in humans

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00034.2007 ◽

2007 ◽

Vol 293 (3) ◽

pp. E690-E696 ◽

Cited By ~ 37

Author(s):

Christoph Pachler ◽

Dimas Ikeoka ◽

Johannes Plank ◽

Heinz Weinhandl ◽

Maria Suppan ◽

...

Keyword(s):

Adipose Tissue ◽

Subcutaneous Adipose Tissue ◽

Fold Increase ◽

Local Effect ◽

Cytokine Response ◽

Open Flow ◽

Tnf Α ◽

Minimal Trauma ◽

Inflammatory Cytokine Response ◽

Subcutaneous Adipose

Inflammatory cytokines released from adipose tissue play an important role in different pathological processes. In the present study, we investigated the inflammatory cytokine response of human subcutaneous adipose tissue (SAT) by applying the open-flow microperfusion technique. Four standard 18-gauge microperfusion catheters were inserted into periumbilical SAT of eight healthy male volunteers [29 ± 3 yr, BMI 24.3 ± 1.9 (mean ± SD)]. SAT probe effluents were collected at 60-min intervals for 8 h after catheter insertion. Different perfusion fluids were used to measure the local effect of insulin and/or glucose on the cytokine response. SAT probe effluents were analyzed for IL-1β, IL-6, CXCL8 (IL-8), and TNF-α. SAT concentrations of IL-1β increased 100-fold from 1.0 ± 0.2 pg/ml (mean ± SE) to 101.5 ± 23.2 pg/ml ( P < 0.001) after 8 h. A 130-fold increase was observed for CXCL8, from 49 ± 29 to 6,554 ± 1,713 pg/ml ( P < 0.001). Furthermore, a 20-fold increase of IL-6 was observed within the first 5 h (from 159 ± 123 to 3,554 ± 394 pg/ml; P < 0.001), and a significant decline to 2,154 ± 216 pg/ml ( P < 0.01) was seen thereafter. Finally, TNF-α increased from 1.4 ± 0.6 to 2.5 ± 0.5 pg/ml ( P < 0.05) in hour 2 and remained stable thereafter. Local administration of insulin exerted a stimulatory effect on the inflammatory response of IL-6. In conclusion, SAT exerts a highly reproducible and consistent proinflammatory cytokine response after minimally invasive trauma caused by the insertion of a catheter in humans.

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