Subcutaneous adipose tissue exerts proinflammatory cytokines after minimal trauma in humans

2007 ◽  
Vol 293 (3) ◽  
pp. E690-E696 ◽  
Author(s):  
Christoph Pachler ◽  
Dimas Ikeoka ◽  
Johannes Plank ◽  
Heinz Weinhandl ◽  
Maria Suppan ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Fold Increase ◽  
Local Effect ◽  
Cytokine Response ◽  
Open Flow ◽  
Tnf Α ◽  
Minimal Trauma ◽  
Inflammatory Cytokine Response ◽  
Subcutaneous Adipose

Inflammatory cytokines released from adipose tissue play an important role in different pathological processes. In the present study, we investigated the inflammatory cytokine response of human subcutaneous adipose tissue (SAT) by applying the open-flow microperfusion technique. Four standard 18-gauge microperfusion catheters were inserted into periumbilical SAT of eight healthy male volunteers [29 ± 3 yr, BMI 24.3 ± 1.9 (mean ± SD)]. SAT probe effluents were collected at 60-min intervals for 8 h after catheter insertion. Different perfusion fluids were used to measure the local effect of insulin and/or glucose on the cytokine response. SAT probe effluents were analyzed for IL-1β, IL-6, CXCL8 (IL-8), and TNF-α. SAT concentrations of IL-1β increased 100-fold from 1.0 ± 0.2 pg/ml (mean ± SE) to 101.5 ± 23.2 pg/ml ( P < 0.001) after 8 h. A 130-fold increase was observed for CXCL8, from 49 ± 29 to 6,554 ± 1,713 pg/ml ( P < 0.001). Furthermore, a 20-fold increase of IL-6 was observed within the first 5 h (from 159 ± 123 to 3,554 ± 394 pg/ml; P < 0.001), and a significant decline to 2,154 ± 216 pg/ml ( P < 0.01) was seen thereafter. Finally, TNF-α increased from 1.4 ± 0.6 to 2.5 ± 0.5 pg/ml ( P < 0.05) in hour 2 and remained stable thereafter. Local administration of insulin exerted a stimulatory effect on the inflammatory response of IL-6. In conclusion, SAT exerts a highly reproducible and consistent proinflammatory cytokine response after minimally invasive trauma caused by the insertion of a catheter in humans.

Open-Flow Microperfusion of Subcutaneous Adipose Tissue for On-Line Continuous Ex Vivo Measurement of Glucose Concentration

Diabetes Care ◽  
1997 ◽  
Vol 20 (7) ◽  
pp. 1114-1121 ◽  
Author(s):  
Z. Trajanoski ◽  
G. A. Brunner ◽  
L. Schaupp ◽  
M. Ellmerer ◽  
P. Wach ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glucose Concentration ◽  
Subcutaneous Adipose Tissue ◽  
Ex Vivo ◽  
Open Flow ◽  
On Line ◽  
Subcutaneous Adipose

GC determination of nicotine in subcutaneous adipose tissue obtained by minimal trauma tissue biopsy

2000 ◽  
Vol 51 (1) ◽  
pp. S237-S240 ◽  
Author(s):  
B. X. Mayer ◽  
M. Brunner ◽  
M. Müller ◽  
H. Mascher ◽  
H. G. Eichler
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Biopsy ◽  
Minimal Trauma ◽  
Subcutaneous Adipose

TNF-α Gene Expression in Subcutaneous Adipose Tissue Associated with HOMA in Asian Indian Postmenopausal Women

2013 ◽  
Vol 46 (02) ◽  
pp. 94-99
Author(s):  
S. Tiwari ◽  
B. Paul ◽  
S. Kumar ◽  
A. Chandra ◽  
S. Dhananjai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Postmenopausal Women ◽  
Asian Indian ◽  
Subcutaneous Adipose Tissue ◽  
Tnf Α ◽  
Subcutaneous Adipose

Expression of TNF-α protein in omental and subcutaneous adipose tissue in obesity

2008 ◽  
Vol 79 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Yan Li Cao ◽  
Cui Zhu Hu ◽  
Xin Meng ◽  
Di Fei Wang ◽  
Jin Zhang
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Tnf Α ◽  
Subcutaneous Adipose

Sex differences in subcutaneous adipose tissue redox homeostasis and inflammation markers in control and high-fat diet fed rats

2019 ◽  
Vol 44 (7) ◽  
pp. 720-726 ◽  
Author(s):  
Renata Prado Vasconcelos ◽  
Milena Simões Peixoto ◽  
Keciany Alves de Oliveira ◽  
Andrea Claudia Freitas Ferreira ◽  
Andrelina Noronha Coelho-de-Souza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Subcutaneous Adipose Tissue ◽  
Redox Homeostasis ◽  
Female Rats ◽  
Mrna Levels ◽  
High Fat ◽  
Tnf Α ◽  
Subcutaneous Adipose

The development of obesity-related metabolic disorders is more evident in male in comparison with female subjects, but the mechanisms are unknown. Several studies have shown that oxidative stress is involved in the pathophysiology of obesity, but the majority of these studies were performed with male animals. The aim of this study was to evaluate the sex-related differences in subcutaneous adipose tissue redox homeostasis and inflammation of rats chronically fed a high-fat diet. NADPH oxidase (NOX), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase activities were evaluated in the subcutaneous adipose tissue (SC) of adult male and female rats fed either a standard chow (SCD) or a high-fat diet (HFD) for 11 weeks. NOX2 and NOX4 messenger RNA (mRNA) levels, total reduced thiols, interleukin (IL)-1β, tumor necrosis factor α (TNF-α), and IL-6 were also determined. Higher antioxidant enzyme activities and total reduced thiol levels were detected in SC of control male compared with female rats. Chronic HFD administration increased NOX activity and NOX2 and NOX4 mRNA levels and decreased SOD and GPx activities only in male animals. IL-1β, TNF-α, and IL-6 levels, as well as Adgre1, CD11b, and CD68 mRNA levels, were also higher in SC of males after HFD feeding. In SC of females, catalase activity was higher after HFD feeding. Taken together, our results show that redox homeostasis and inflammation of SC is sexually dimorphic. Furthermore, males show higher oxidative stress in SC after 11 weeks of HFD feeding owing to both increased reactive oxygen species (ROS) production through NOX2 and NOX4 and decreased ROS detoxification.

Production of soluble tumor necrosis factor receptors by human subcutaneous adipose tissue in vivo

1999 ◽  
Vol 277 (6) ◽  
pp. E971-E975 ◽  
Author(s):  
Vidya Mohamed-Ali ◽  
Steven Goodrick ◽  
Karen Bulmer ◽  
Jeffrey M. P. Holly ◽  
John S. Yudkin ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Body Fat ◽  
Subcutaneous Adipose Tissue ◽  
Tnf Receptor ◽  
Soluble Receptor ◽  
Tnf Α ◽  
Subcutaneous Adipose ◽  
Tissue Production ◽  
Necrosis Factor

To investigate in vivo adipose tissue production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and their soluble receptors: TNF receptor type I (sTNFR-I), TNF receptor type II (sTNFR-II), and IL-6 receptor (sIL-6R), we determined arteriovenous differences in their levels across abdominal subcutaneous adipose tissue in obese subjects. Subjects had a median (interquartile range) age of 44.5 (27–51.3) yr, body mass index (BMI) of 32.9 (26.0–46.6) kg/m2, and %body fat of 42.5 (28.5–51.2) %. Although there was not a significant difference in the arteriovenous concentrations of TNF-α ( P = 0.073) or sTNFR-II ( P = 0.18), the levels of sTNFR-I ( P = 0.002) were higher in the vein compared with artery, suggesting adipose tissue production of this soluble receptor. There was a significant arteriovenous difference in IL-6 ( P < 0.001) but not in its soluble receptor ( P = 0.18). There was no relationship between TNF-α levels and adiposity indexes ( r s = 0.12–0.22, P = not significant); however, levels of both its soluble receptor isomers correlated significantly with BMI and %body fat (sTNFR-I r s = 0.42–0.72, P < 0.001; sTNFR-II r s = 0.36–0.65, P < 0.05- <0.001). IL-6 levels correlated significantly with both BMI and %body fat ( r s = 0.51, P = 0.004, and r s = 0.63, P < 0.001), but sIL-6R did not. In conclusion, 1) soluble TNFR-I is produced by adipose tissue, and concentrations of both soluble isoforms correlate with the degree of adiposity, and 2) IL-6, but not its soluble receptor, is produced by adipose tissue and relates to adiposity.

TNF-α, but not IL-6, stimulates plasminogen activator inhibitor-1 expression in human subcutaneous adipose tissue

2005 ◽  
Vol 98 (6) ◽  
pp. 2019-2023 ◽  
Author(s):  
Peter Plomgaard ◽  
Pernille Keller ◽  
Charlotte Keller ◽  
Bente Klarlund Pedersen
Keyword(s):  
Adipose Tissue ◽  
Mrna Expression ◽  
Plasminogen Activator ◽  
Subcutaneous Adipose Tissue ◽  
Plasminogen Activator Inhibitor ◽  
Plasminogen Activator Inhibitor 1 ◽  
Tnf Α ◽  
Subcutaneous Adipose ◽  
Activator Inhibitor ◽  
Pai 1

Plasminogen activator inhibitor-1 (PAI-1) is produced by adipose tissue, and elevated PAI-1 levels in plasma are a risk factor in the metabolic syndrome. We investigated the regulatory effects of TNF-α and IL-6 on PAI-1 gene induction in human adipose tissue. Twenty healthy men underwent a 3-h infusion of either recombinant human TNF-α ( n = 8), recombinant human IL-6 ( n = 6), or vehicle ( n = 6). Biopsies were obtained from the subcutaneous abdominal adipose tissue at preinfusion, at 1, 2, and 3 h during the infusion, and at 2 h after the infusion. The mRNA expression of PAI-1 in the adipose tissue was measured using real-time PCR. The plasma levels of TNF-α and IL-6 reached 18 and 99 pg/ml, respectively, during the infusions. During the TNF-α infusion, adipose PAI-1 mRNA expression increased 2.5-fold at 1 h, 6-fold at 2 h, 9-fold at 3 h, and declined to 2-fold 2 h after the infusion stopped but did not change during IL-6 infusion and vehicle. These data demonstrate that TNF-α rather than IL-6 stimulates an increase in PAI-1 mRNA in the subcutaneous adipose tissue, suggesting that TNF-α may be involved in the pathogenesis of related metabolic disorders.

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