A method for isolating large numbers of viable disaggregated cells from various human tissues for cell culture establishment

1986 ◽  
Vol 22 (9) ◽  
pp. 529-534 ◽  
Author(s):  
Ruth E. Gibson-D'ambrosio ◽  
Mervyn Samuel ◽  
Steven M. D'Ambrosio
Keyword(s):  
Cell Culture ◽  
Human Tissues ◽  
Large Numbers

Patterned superhydrophobic surfaces to process and characterize biomaterials and 3D cell culture

2018 ◽  
Vol 5 (3) ◽  
pp. 379-393 ◽  
Author(s):  
A. I. Neto ◽  
P. A. Levkin ◽  
J. F. Mano
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Superhydrophobic Surfaces ◽  
Technological Breakthrough ◽  
Large Numbers

Microarrays are a technological breakthrough for high-throughput screening of large numbers of assays.

scholarly journals Generalized Equine Cutaneous Mastocytosis

1972 ◽  
Vol 9 (6) ◽  
pp. 394-407 ◽  
Author(s):  
N. F. Cheville ◽  
K. Prasse ◽  
M. van der Maaten ◽  
A. D. Boothe
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Mast Cells ◽  
Skin Lesions ◽  
Eosinophil Infiltration ◽  
Equine Herpesvirus ◽  
Cytoplasmic Granules ◽  
Large Numbers ◽  
Cutaneous Mast Cell ◽  
Cutaneous Mastocytosis

A newborn foal developed generalized cutaneous mastocytosis characterized by multiple elevated nodules of mast cells in skin and basophil hyperplasia in bone marrow. Skin lesions began as small aggregates of mast cells that progressively enlarged, ulcerated, and regressed spontaneously. Eosinophil infiltration, collagen necrosis, and fibroplasia were characteristic of advanced lesions. Many new lesions developed during the first month of life but numbers progressively diminished. Large numbers of mast cells were present in biopsies of lymph node, spleen and bone marrow. Discrete aggregates of mast cells were present in the bone marrow postmortem but no other significant change was seen. Mast cells contained large amounts of histamine but little serotonin. Ultrastructurally, their cytoplasmic granules were chiefly granular with few dense forms. In cell culture, mast cells from early lesions maintained mitotic activity through 14 passages. Cells obtained from older lesions were rapidly overgrown with fibroblasts. An equine herpesvirus isolated from cultures of cutaneous mast cell lesions and of spleen was not thought to be related to the disease.

scholarly journals THE RELATIVE EXTENSIBILITY OF CELL SURFACES

1963 ◽  
Vol 17 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Murray D. Rosenberg
Keyword(s):  
Cell Culture ◽  
Mechanical Deformation ◽  
Culture Chamber ◽  
Cell Surfaces ◽  
Statistical Index ◽  
Degree Of Deformation ◽  
Large Numbers ◽  
Dissociated Cells ◽  
Definition Of

Observations have been made on the response, in vitro, of cultured and freshly dissociated cells to mechanical deformation. Large numbers of individual cells were studied by means of a special culture chamber bounded by two parallel glass coverslips whose spacing could be reduced from 140 to 2 microns in steps of roughly 0.5 micron. The degree of deformation required for herniation of the cell surface was measured. These measurements lead to the definition of a statistical index characteristic of the extensibility of cell surfaces. This index has been shown to be distinctive for several types of cells; to alter with certain stages of embryonic development; and to be stable with respect to the culturing of cells and certain alterations in the method of cell culture.

Collection of human tissues for use in cell culture research

1979 ◽  
Vol 5 (3) ◽  
pp. 1099-1106
Author(s):  
Robert J. Hay
Keyword(s):  
Cell Culture ◽  
Human Tissues

scholarly journals Genomic Research with the Newly Dead: A Crossroads for Ethics and Policy

2014 ◽  
Vol 42 (2) ◽  
pp. 220-231 ◽  
Author(s):  
Rebecca L. Walker ◽  
Eric T. Juengst ◽  
Warren Whipple ◽  
Arlene M. Davis
Keyword(s):  
Genomic Medicine ◽  
Tissue Expression ◽  
National Institutes Of Health ◽  
Genomic Research ◽  
Genomic Sequencing ◽  
Human Tissues ◽  
Large Numbers ◽  
Heart Beating ◽  
Interest In Research ◽  
The Relationship

Research uses of human bodies maintained by mechanical ventilation after being declared dead by neurological criteria (“heart-beating cadavers”), were first published in the early 1980s with a renewed interest in research on the newly or nearly dead occurring in about last decade. While this type of research may take many different forms, recent technologic advances in genomic sequencing along with high hopes for genomic medicine, have inspired interest in genomic research with the newly dead. For example, the Genotype-Tissue Expression (GTEx) program through the National Institutes of Health aims to collect large numbers of diverse human tissues with the eventual goal of elucidating the genetic bases of common diseases through a better understanding of the relationship between genetic variation and gene expression.

scholarly journals Opportunities for organoids as new models of aging

2017 ◽  
Vol 217 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Jennifer L. Hu ◽  
Michael E. Todhunter ◽  
Mark A. LaBarge ◽  
Zev J. Gartner
Keyword(s):  
Cell Culture ◽  
Animal Studies ◽  
Model Organisms ◽  
Human Tissues ◽  
Human Cell Culture ◽  
Aging Research ◽  
New Models ◽  
Culture Models ◽  
Biology Of Aging ◽  
Cell Culture Models

The biology of aging is challenging to study, particularly in humans. As a result, model organisms are used to approximate the physiological context of aging in humans. However, the best model organisms remain expensive and time-consuming to use. More importantly, they may not reflect directly on the process of aging in people. Human cell culture provides an alternative, but many functional signs of aging occur at the level of tissues rather than cells and are therefore not readily apparent in traditional cell culture models. Organoids have the potential to effectively balance between the strengths and weaknesses of traditional models of aging. They have sufficient complexity to capture relevant signs of aging at the molecular, cellular, and tissue levels, while presenting an experimentally tractable alternative to animal studies. Organoid systems have been developed to model many human tissues and diseases. Here we provide a perspective on the potential for organoids to serve as models for aging and describe how current organoid techniques could be applied to aging research.

scholarly journals Spatial expression patterns of peptide transporters in the human and rat gastrointestinal tracts, Caco-2 In Vitro cell culture model, and multiple human tissues

AAPS PharmSci ◽  
2001 ◽  
Vol 3 (1) ◽  
pp. 100-111 ◽  
Author(s):  
Dea Herrera-Ruiz ◽  
Qing Wang ◽  
Thomas J. Cook ◽  
Gregory T. Knipp ◽  
Olafur S. Gudmundsson ◽  
...  
Keyword(s):  
Cell Culture ◽  
Expression Patterns ◽  
Human Tissues ◽  
Cell Culture Model ◽  
Spatial Expression ◽  
In Vitro Cell Culture ◽  
Peptide Transporters ◽  
Culture Model

scholarly journals MipTec Proceedings: A Silicon-Based Compact Cell Culture Disc

1998 ◽  
Vol 3 (4) ◽  
pp. 58-62
Author(s):  
B. Larsson ◽  
N. Crompton ◽  
J. Gobrecht ◽  
C. Higgs ◽  
G. Kuehne ◽  
...  
Keyword(s):  
Cell Culture ◽  
Spectroscopic Analysis ◽  
Single Cells ◽  
Chemical Parameters ◽  
Rapid Succession ◽  
Physical Chemical ◽  
Large Numbers ◽  
Cell Clones ◽  
Computer Aided ◽  
Silicon Based

A microplate technology based on a Compact Cell Culture Disc (CCCD) is described. This new tool employs a multitude of miniature cell culture chambers set into a planar compact disc suitable for computer-aided microscopic or spectroscopic analysis of single cells or cell clones in large numbers. The aim is to perform automated, accurate and reproducible studies of morphologic and physical-chemical parameters, in rapid succession under physiologically and optically ideal, uniform and reproducible conditions.

Bovine Pulmonary, Hepatic and Renal Tissues: Models for the Study of Mammalian C-S Lyase Enzymes

1993 ◽  
Vol 21 (3) ◽  
pp. 360-370
Author(s):  
Lorraine D. Buckberry ◽  
Ian S. Blagbrough ◽  
Barrie W. Bycroft ◽  
P. Nicholas Shaw
Keyword(s):  
Laboratory Animals ◽  
Human Tissues ◽  
Large Numbers ◽  
Bovine Tissue ◽  
Reasonable Model

C-S lyase (CSL) enzymes are responsible for the generation of toxicity via the cleavage of cysteine conjugates to generate reactive thiol species. In order to explore and characterise CSL activity in mammalian organs, cysteine conjugate CSL enzymes were isolated from bovine pulmonary, hepatic and renal tissues. Bovine tissue”, obtained from the abbatoir, affords a readily available source of viable CSL enzymes, without the necessity of sacrificing large numbers of laboratory animals simply to provide tissue. We have demonstrated that significant CSL activity exists in bovine tissues, and that the level of this activity is comparable with that found in human tissues. These enzymes provide an explanation for the previously reported episodes of bovine toxicity, and may provide a reasonable model for other mammalian CSL enzymes.

scholarly journals Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics

2022 ◽  
Vol 5 (1) ◽  
Author(s):  
Kenichiro Ito ◽  
Yoshihiko Matsuda ◽  
Ayako Mine ◽  
Natsuki Shikida ◽  
Kazutoshi Takahashi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Growth Factors ◽  
Growth Factor ◽  
Regenerative Medicine ◽  
Corynebacterium Glutamicum ◽  
Disulfide Bonds ◽  
Biologically Active ◽  
Single Chain ◽  
Large Numbers ◽  
Macrocyclic Peptides

AbstractMimetics of growth factors and cytokines are promising tools for culturing large numbers of cells and manufacturing regenerative medicine products. In this study, we report single-chain tandem macrocyclic peptides (STaMPtides) as mimetics in a new multivalent peptide format. STaMPtides, which contain two or more macrocyclic peptides with a disulfide-closed backbone and peptide linkers, are successfully secreted into the supernatant by Corynebacterium glutamicum-based secretion technology. Without post-secretion modification steps, such as macrocyclization or enzymatic treatment, bacterially secreted STaMPtides form disulfide bonds, as designed; are biologically active; and show agonistic activities against respective target receptors. We also demonstrate, by cell-based assays, the potential of STaMPtides, which mimic growth factors and cytokines, in cell culture. The STaMPtide technology can be applied to the design, screening, and production of growth factor and cytokine mimetics.

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