Bacterial and fungal communities experience rapid succession during the first year following a wildfire in a California chaparral.

The rise in wildfire frequency in the western United States has increased interest in secondary succession. However, despite the role of soil microbial communities in plant regeneration and establishment, microbial secondary succession is poorly understood owing to a lack of measurements immediately post-fire and at high temporal resolution. To fill this knowledge gap, we collected soils at 2 and 3 weeks and 1, 2, 3, 4, 6, 9, and 12 months after a chaparral wildfire in Southern California. We assessed bacterial and fungal biomass with qPCR of 16S and 18S and richness and composition with Illumina MiSeq sequencing of the 16S and ITS2 amplicons. We found that fire severely reduced bacterial biomass by 47% and richness by 46%, but the impacts were stronger for fungi, with biomass decreasing by 86% and richness by 68%. These declines persisted for the entire post-fire year, but bacterial biomass and richness oscillated in response to precipitation, whereas fungal biomass and richness did not. Fungi and bacteria experienced rapid succession, with 5-6 compositional turnover periods. As with plants, fast-growing surviving microbes drove successional dynamics. For bacteria, succession was driven by the phyla Firmicutes and Proteobacteria, with the Proteobacteria Massilia dominating all successional time points, and the Firmicutes (Domibacillus and Paenibacillus) dominating early- to mid-successional stages (1-4.5 months), while the Proteobacteria Noviherbaspirillum dominated late successional stages (4.5-1 year). For fungi, succession was driven by the phyla Ascomycota, but ectomycorrhizal basidiomycetes, and the heat-resistant yeast, Geminibasidium were present in the early successional stages (1 month). However, pyrophilous filamentous Ascomycetes Pyronema, Penicillium, and Aspergillus, dominated all post-fire time points. While wildfires vastly decrease bacterial and fungal biomass and richness, similar to plants, pyrophilous bacteria and fungi increase in abundance and experience rapid succession and compositional turnover in the first post-fire year, with potential implications for post-fire chaparral regeneration

Grouping successive freezing of gait episodes has neutral to detrimental effect on freeze detection and prediction in Parkinson’s disease

Freezing of gait (FOG) is an intermittent walking disturbance experienced by people with Parkinson’s disease (PD). Wearable FOG identification systems can improve gait and reduce the risk of falling due to FOG by detecting FOG in real-time and providing a cue to reduce freeze duration. However, FOG prediction and prevention is desirable. Datasets used to train machine learning models often generate ground truth FOG labels based on visual observation of specific lower limb movements (event-based definition) or an overall inability to walk effectively (period of gait disruption based definition). FOG definition ambiguity may affect model performance, especially with respect to multiple FOG in rapid succession. This research examined whether merging multiple freezes that occurred in rapid succession could improve FOG detection and prediction model performance. Plantar pressure and lower limb acceleration data were used to extract a feature set and train decision tree ensembles. FOG was labeled using an event-based definition. Additional datasets were then produced by merging FOG that occurred in rapid succession. A merging threshold was introduced where FOG that were separated by less than the merging threshold were merged into one episode. FOG detection and prediction models were trained for merging thresholds of 0, 1, 2, and 3 s. Merging slightly improved FOG detection model performance; however, for the prediction model, merging resulted in slightly later FOG identification and lower precision. FOG prediction models may benefit from using event-based FOG definitions and avoiding merging multiple FOG in rapid succession.

Tokyo will move closer to Washington on Taiwan

Significance In rapid succession, unprecedented statements, meetings and actions over the last few months indicate stronger Japanese support for Taiwan, and by implication its autonomy from China. Impacts Taiwan’s accession to the CPTPP is still unlikely as long as China opposes it and Taiwan continues banning certain Japanese food imports. Industries depending on high-end semiconductors will benefit from Japan’s diversification of its semiconductor supply chains. Though he presents himself as tough on China, Japan's new prime minister may turn out to be more risk-averse on Taiwan.

“Faith so as to Remove Mountains”

The presence of πίστις in the list of spiritual gifts in1 Cor 12:8-10 is problematic. Should not faith be the common basis of the charisms and not a particular gift? What means “faith” in 1 Cor 12:9, given that Paul includes it among the nine manifestations of the Spirit? How to understand and to translate it? Evidently, “faith” points here not simple adherence, but a very precise form of πίστις. In fact, Paul places wisdom, knowledge and “faith” in rapid succession, making them the three principal dimensions of the teaching charisms. As reflected in the repetition of the same gifts in the sequence of 13:2. Some Jewish texts talk about rabbis able to change the point of view of people whose convictions were as immovable as a mountain. They were nicknamed “mountain mover” (oker harim). Thanks to the support of rabbinic literature it becomes possible to explain the presence of πίστις among the teaching gifts in 1 Cor 12:9 and 13:2, offering a translation, certainly broad but more attentive to the semantics of the charism of faith: “if I have all the ability to deliver discourses of faith from which to convince people as immovable as mountains, but I do not have charity, I am nothing”.

Can the Subaltern Speak?

This paper presents AI-controlled robotic masks intended to empower women and allow them to communicate with one another. These are inspired by the historical masks worn by the Bandari women from southern Iran. Legend has it that these masks were developed during Portuguese colonial rule as a way of protecting the wearer from the gaze of slave masters looking for attractive women. In this project two robotic masks seemingly begin to develop their own language to communicate with each other, blinking their eyelashes in rapid succession using AI-generated Morse code. This project draws upon a Facebook experiment where two AI bots apparently began to develop their own language. It also draws upon an incident when an American soldier used his eyes to blink and spell out the word "TORTURE" using Morse code during his captivity in Vietnam, as well as stories of women using code to report domestic abuse during the COVID-19 lockdown. The aim is to sow anxiety within the patriarchal system where the "wink" of the sexual predator is subverted into a language to protect women from the advances of a predator. The project bridges AI, interactive design, and critical thinking (Figure 1).

Adaptable and Automated Rodent Behavior Maze System

Mazes are a fundamental and widespread tool in behavior and systems neuroscience research in rodents. However, their form and inflexibility often restrict potential experimental paradigms that involve multiple or adaptive maze designs. Unique layouts often cost substantial engineering and time investments from trainee scientists. To alleviate these issues, we have developed an automated modular maze system that is flexible and scalable. This system will allow for experiments with multiple track configurations in rapid succession. Additionally, the flexibility can expedite prototyping of behaviors. Finally, the standardized componentry enhances experimental reproducibility and repeatability. This maze system presents advantages over current maze options and can facilitate novel behavior and systems neuroscience research.

ESCA pipeline: Easy-to-use SARS-CoV-2 genome Assembler

Early sequencing and quick analysis of SARS-CoV-2 genome are contributing to un-derstand the dynamics of COVID19 epidemics and to countermeasures design at global level. Amplicon-based NGS methods are widely used to sequence the SARS-CoV-2 genome and to identify novel variants that are emerging in rapid succession, harboring multiple deletions and amino acid changing mutations. To facilitate the analysis of NGS sequencing data obtained from amplicon-based sequencing methods, here we propose an easy-to-use SARS-CoV-2 genome Assembler: the ESCA pipeline. Results showed that ESCA can perform high quality genome assembly from IonTor-rent and Illumina raw data, and help the user in easily correct low-coverage regions. Moreover, ESCA includes the possibility to compare assembled genomes of multi sample runs through an easy table format.

Processing of facial expressions of same-race and other-race faces: distinct and shared neural underpinnings

People understand others’ emotions quickly from their facial expressions. However, facial expressions of ingroup and outgroup members may signal different social information and thus be mediated by distinct neural activities. We investigated whether there are distinct neuronal responses to fearful and happy expressions of same-race (SR) and other-race (OR) faces. We recorded electroencephalogram from Chinese adults when viewing an adaptor face (with fearful/neutral expressions in Experiment 1 but happy/neutral expressions in Experiment 2) and a target face (with fearful expressions in Experiment 1 but happy expressions in Experiment 2) presented in rapid succession. We found that both fearful and happy (vs neutral) adaptor faces increased the amplitude of a frontocentral positivity (P2). However, a fearful but not happy (vs neutral) adaptor face decreased the P2 amplitudes to target faces, and this repetition suppression (RS) effect occurred when adaptor and target faces were of the same race but not when of different races. RS was observed on two late parietal/central positive activities to fearful/happy target faces, which, however, occurred regardless of whether adaptor and target faces were of the same or different races. Our findings suggest that early affective processing of fearful expressions may engage distinct neural activities for SR and OR faces.

Los trabajos de Persiles y Sigismunda

Published posthumously, Los trabajos de Persiles y Sigismunda: Historia setentrional (1617) was the triumphant capstone to a remarkable run of works written, revised, or concluded in the wake of the first part of Don Quixote (1605). Cervantes would leave his inventive stamp on every major genre of literary entertainment then fashionable: myriad verse forms, drama, pastoral and chivalric novels, the novella, the Menippean satirical dream, and the Heliodoran adventure novel, along with others as interpolated tale, incident, or passing characterization. The last four years of his life, which saw a rapid succession of publications, suggest a veteran writer used to taking his time but now acutely aware it was running out. Proud of the 1605 Quixote’s wild popularity, Cervantes was also anxious to shape his literary legacy so that it would not be swallowed up by its run-away success.