Effects of potassium on the ethanol production rate of Saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation

1992 ◽  
Vol 37 (1) ◽  
pp. 1-10 ◽  
Author(s):  
N. L. C. Licinio Da Silva ◽  
A. A. Salgueiro ◽  
W. M. Ledingham ◽  
E. H. M. Melo ◽  
J. L. Lima Filho

2013 ◽  
Vol 805-806 ◽  
pp. 281-285
Author(s):  
Zhong Xu

Bioconversion of potato pulp to fuel ethanol, analysing the potato pulp chemical composition and determining the potato pulp in the role of microorganism produce ethanol under the best conditions is the major research. An analysis of the chemical composition of potato pulp showed that : the basic ingredients are Protein (9.72%), Starch (25.52%), Cellulose (17.90%). The effects of ethanol production rate of solid-liquid ratio, fermentation temperature, inoculumconcertration, fermentation time. The results showed that: the best conditions producting ethanol from potato pulp obtained by single factor experiments are: solid-liquid ratio: 1:15, fermentation temperature: 35°C, inoculumconcertration: 3mL, fermentation time: 20h. Under this occasion, the ethanol production rate was 0.183mL·g-1.



2008 ◽  
Vol 15 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Gerardo Huerta-Beristain ◽  
José Utrilla ◽  
Georgina Hernández-Chávez ◽  
Francisco Bolívar ◽  
Guillermo Gosset ◽  
...  


2011 ◽  
Vol 31 (No. 1) ◽  
pp. 1-6
Author(s):  
J. Goliáš ◽  
A. Němcová ◽  
L. Šuderlová

Plum fruits of the cultivars Stanley and Valjevka picked at the beginning of climacteric were stored in different storage atmospheres for 31 days at 3&deg;C. The relations between the O<sub>2</sub> and CO<sub>2</sub> content during this period and after removal from the gas mixture to ethanol, acetaldehyde, non-volatile compounds and some textural values of fruits were investigated. Concentrations of ethanol in the flesh were related to levels of oxygen and CO<sub>2</sub> in ambient atmosphere. In anaerobic conditions (&lt; 0.2% O<sub>2</sub>) ethanol reached 1,109 mg/l for the cultivar Valjevka and 628 mg/l for Stanley. The results of single fruit analysis showed a steeply increasing concave curve of ethanol production in the period of anaerobic conditions, followed by the phase of a drop of the production rate in air stored fruit. The concentration of oxygen at a level of 0.9% (ultra low oxygen &ndash; ULO) does not physiologically harm the tissues of plums by producing mostly negligible content of ethanol and acetaldehyde, but an ethanol increase to half concentration after 31 days was observed to compare with anaerobic conditions in the cultivar Valjevka. From this aspect plums seem to be relatively sensitive to low oxygen. The post-storage period was extended up to 53 to 63 days, respectively. The senescence caused an increase in ethanol production rate that was exponentially increased after 20 days of cold storage atmosphere. The final concentration after 53 days was still higher for cv. Valjevka than for cv. Stanley at the respective content of 828 mg/land 498 mg/l. Skin firmness was differentiated for both cultivars, and softness was higher for the cultivar Valjevka. &nbsp;



2021 ◽  
Author(s):  
Zhongshan Yang ◽  
Qiqi Zhang ◽  
Liteng Ren ◽  
Xin Chen ◽  
Defa Wang ◽  
...  

An ethanol production rate as high as 281.6 μmol g−1 h−1 was achieved during the photocatalytic conversion of CH4 in the presence of O2 at room temperature.



2003 ◽  
Vol 69 (6) ◽  
pp. 3251-3257 ◽  
Author(s):  
Elisabeth Thomsson ◽  
Christer Larsson ◽  
Eva Albers ◽  
Annika Nilsson ◽  
Carl Johan Franz�n ◽  
...  

ABSTRACT Seven different strains of Saccharomyces cerevisiae were tested for the ability to maintain their fermentative capacity during 24 h of carbon or nitrogen starvation. Starvation was imposed by transferring cells, exponentially growing in anaerobic batch cultures, to a defined growth medium lacking either a carbon or a nitrogen source. After 24 h of starvation, fermentative capacity was determined by addition of glucose and measurement of the resulting ethanol production rate. The results showed that 24 h of nitrogen starvation reduced the fermentative capacity by 70 to 95%, depending on the strain. Carbon starvation, on the other hand, provoked an almost complete loss of fermentative capacity in all of the strains tested. The absence of ethanol production following carbon starvation occurred even though the cells possessed a substantial glucose transport capacity. In fact, similar uptake capacities were recorded irrespective of whether the cells had been subjected to carbon or nitrogen starvation. Instead, the loss of fermentative capacity observed in carbon-starved cells was almost surely a result of energy deprivation. Carbon starvation drastically reduced the ATP content of the cells to values well below 0.1 μmol/g, while nitrogen-starved cells still contained approximately 6 μmol/g after 24 h of treatment. Addition of a small amount of glucose (0.1 g/liter at a cell density of 1.0 g/liter) at the initiation of starvation or use of stationary-phase instead of log-phase cells enabled the cells to preserve their fermentative capacity also during carbon starvation. The prerequisites for successful adaptation to starvation conditions are probably gradual nutrient depletion and access to energy during the adaptation period.





2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Wei Han ◽  
Zhanqing Wang ◽  
Hong Chen ◽  
Xin Yao ◽  
Yongfeng Li

The effects of organic loading rates (OLRs) on fermentative productions of hydrogen and ethanol were investigated in a continuous stirred tank reactor (CSTR) with attached sludge using molasses as substrate. The CSTR reactor with attached sludge was operated under different OLRs, ranging from 8 to 24 kg/m3·d. The H2and ethanol production rate essentially increased with increasing OLR. The highest H2production rate (10.74 mmol/hL) and ethanol production rate (11.72 mmol/hL) were obtained both operating at OLR = 24 kg/m3·d. Linear regression results show that ethanol production rate () and H2production rate () were proportionately correlated and can be expressed as (). The best energy generation rate was 19.08 kJ/hL, which occurred at OLR = 24 kg/m3·d. In addition, the hydrogen yield was affected by the presence of ethanol and acetic acid in the liquid phase, and the maximum hydrogen production rate occurred while the ratio of ethanol to acetic acid was close to 1.



2020 ◽  
Vol 66 (4) ◽  
pp. 296-306
Author(s):  
Radim Holesinsky ◽  
Bozena Prusova ◽  
Mojmir Baron ◽  
Jaromir Fiala ◽  
Eliska Sembolova ◽  
...  

In this paper we focus on possibilities of using natural microflora from vineyards to enhance spontaneous fermentation. Obtained yeast isolates, naturally occurring in vineyards, which were previously isolated in 2017 and 2018, were used for inoculation of Hibernal grape must. Spontaneously fermented musts and musts with the yeast isolates from 2017 and 2018 were compared. Basic parameters of the musts (sugar concentration, pH, concentration of titratable acids, concentration of assimilated nitrogen) were analysed studied, and a sensory analysis of the resultant wines was performed. The fermentation with the yeast isolates was quick and smooth. In the spontaneous fermentation lower ethanol production rate was observed at the end of the fermentation process. During the sensory evaluation, fruitiness of the spontaneously fermented batch was lower, but its vegetal characteristic was pronounced. The variant with the yeast isolates from 2017 was described as smooth, and the variant with yeast isolates from 2018 was evaluated as slightly vegetal.



2021 ◽  
Vol 333 ◽  
pp. 1-9
Author(s):  
Yu Rim Park ◽  
Ji Won Yang ◽  
In Yung Sunwoo ◽  
Byeong-Kwan Jang ◽  
Soo Rin Kim ◽  
...  


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