Classification of cells in vaginal smears with television microscopy

1976 ◽  
Vol 20 (1) ◽  
pp. 205-216
Author(s):  
J. Blomster ◽  
B. Stenkvist

1954 ◽  
Vol 11 (3) ◽  
pp. 277-284 ◽  
Author(s):  
J. D. BIGGERS ◽  
P. J. CLARINGBOLD

SUMMARY By means of discriminant analysis the validity of a fourfold qualitative classification of vaginal smears has been examined in two inbred lines of mice and their reciprocal crosses. For both the intravaginal and subcutaneous routes of administration with oestradiol-3:17β the vaginal response is shown to be truly quantal, no graded classification of responses being necessary. It is shown that prooestrous smears should be classified as positive and also that the presence or absence of leucocytes is the best criterion for classification.



1980 ◽  
Vol 167 (2-4) ◽  
pp. 247-253 ◽  
Author(s):  
J. Rowiński ◽  
C. Souchier ◽  
R. Fages ◽  
J.L. Laurent ◽  
J.C. Czyba


2007 ◽  
Vol 196 (6) ◽  
pp. 528.e1-528.e6 ◽  
Author(s):  
Hans Verstraelen ◽  
Rita Verhelst ◽  
Kristien Roelens ◽  
Geert Claeys ◽  
Steven Weyers ◽  
...  


1979 ◽  
Vol 27 (1) ◽  
pp. 567-572 ◽  
Author(s):  
K Goerttler ◽  
M Stöhr

Fifty-one gynecologic specimens were collected from three women's hospitals and mailed in a prefixed status to our laboratory. The specimens were classified into a negative, a suspicious, a postradiation, and a positive group. After single cell dispersion the samples were stained for DNA and protein, analyzed, and sorted in the dual laser equipped Heidelberg flow analyzer sorter (HEIFAS). Particles with elevated DNA values (beyond 3.5 ploidy) and with intermediate protein values were sorted as the positive fraction directly on microscopic slides. After restaining according to Papanicolaou, they were re-evaluated cytologically and identified as tumor cells, dysplastic cells and false alarms. The latter consist of doublets and aggregates of more than two cells, binucleated cells, sperm aggregates and epithelial cells contaminated with bacteria. The different groups showed significant differences regarding the total rate of aggregates to single cells. In general, false alarms were very frequent in the positive region and impeded the statistical classification of the sample. The reduction of false alarms is a prerequisite for prescreening with flow instrumentation.



1959 ◽  
Vol 18 (4) ◽  
pp. 359-365 ◽  
Author(s):  
M. F. McDONALD ◽  
J. I. RAESIDE

SUMMARY Three series of experiments were conducted with ovariectomized Romney ewes to determine the requirements of oestradiol benzoate (ODB) for oestrus, characteristic vaginal smear changes, and crystallization (arborization) of cervical mucus, when preceded by 3 days of progesterone treatment (10 mg/day). The respective values for ED50 of ODB (in μg) for oestrus, vaginal smear changes and arborization of cervical mucus, with the associated 95% fiducial limits, were 26·5–23·4–20·9, 15·4–13·2–11·8 and 12·2–7·0–5·2. In the second series of experiments, conducted during November and December, the oestrous response was negligible. During the experimental period February to May, when the first and third series of trials were performed, a greater and more consistent response was noted. The possibility of a seasonal effect in the spayed ewe is discussed. The use of vaginal smears and arborization of cervical mucus in quantitative studies with progesterone and oestrogen was considered. Comparison of cervical mucus and vaginal smears collected from ewes treated with a dose of 10 μg ODB showed more distinctive changes in the cervical mucus and easier classification of a response to oestrogen. The difficulty of collecting a satisfactory sample of cervical mucus is discussed.



1972 ◽  
Vol 128 (6) ◽  
pp. 301-310 ◽  
Author(s):  
D.W. Christie ◽  
J.B. Bailey ◽  
E.T. Bell


1966 ◽  
Vol 24 ◽  
pp. 21-23
Author(s):  
Y. Fujita

We have investigated the spectrograms (dispersion: 8Å/mm) in the photographic infrared region fromλ7500 toλ9000 of some carbon stars obtained by the coudé spectrograph of the 74-inch reflector attached to the Okayama Astrophysical Observatory. The names of the stars investigated are listed in Table 1.



Author(s):  
Gerald Fine ◽  
Azorides R. Morales

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)



Author(s):  
Irving Dardick

With the extensive industrial use of asbestos in this century and the long latent period (20-50 years) between exposure and tumor presentation, the incidence of malignant mesothelioma is now increasing. Thus, surgical pathologists are more frequently faced with the dilemma of differentiating mesothelioma from metastatic adenocarcinoma and spindle-cell sarcoma involving serosal surfaces. Electron microscopy is amodality useful in clarifying this problem.In utilizing ultrastructural features in the diagnosis of mesothelioma, it is essential to appreciate that the classification of this tumor reflects a variety of morphologic forms of differing biologic behavior (Table 1). Furthermore, with the variable histology and degree of differentiation in mesotheliomas it might be expected that the ultrastructure of such tumors also reflects a range of cytological features. Such is the case.



Author(s):  
Paul DeCosta ◽  
Kyugon Cho ◽  
Stephen Shemlon ◽  
Heesung Jun ◽  
Stanley M. Dunn

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).



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