Free urinary cortisol radioimmunoassay

1978 ◽  
Vol 8 (3) ◽  
Author(s):  
Marco Boscaro ◽  
Isabella Karbowiak ◽  
Franco Mantero
Keyword(s):  
Urinary Cortisol ◽  
Free Urinary Cortisol

URINARY EXCRETION OF FREE CORTISOL IN HYPERCALCAEMIA

1973 ◽  
Vol 74 (1) ◽  
pp. 122-126 ◽  
Author(s):  
F. Schønau Jorgensen ◽  
H. Kehlet
Keyword(s):  
Urinary Excretion ◽  
Hpa Axis ◽  
Animal Studies ◽  
Urinary Cortisol ◽  
Free Cortisol ◽  
Free Urinary Cortisol ◽  
Urinary Cortisol Excretion ◽  
Cortisol Excretion

ABSTRACT Human and animal studies have uniformly demonstrated increased hypothalamic-pituitary-adrenocortical (HPA) activity during acute hypercalcaemia. The HPA-activity during chronic hypercalcaemia was investigated by means of free urinary cortisol excretion. No difference in HPA activity could be demonstrated between a hyperparathyroid hypercalcaemic and a normocalcaemic group of patients. Based on these results it is suggested that during chronic hypercalcaemia, the HPA feed back mechanism overcomes the influence of hypercalcaemia on the HPA-axis.

Free Urinary Cortisol Immunoreactive Levels in Premature and Full Term Infants

1986 ◽  
Vol 75 (6) ◽  
pp. 919-922 ◽  
Author(s):  
A. SIAMOPOULOU-MAVRIDOU ◽  
A. K. MAVRIDIS ◽  
A. VIZANDIADIS ◽  
P. HARSOULIS
Keyword(s):  
Full Term ◽  
Urinary Cortisol ◽  
Term Infants ◽  
Free Urinary Cortisol

URINARY EXCRETION OF FREE CORTISOL IN IMPAIRED RENAL FUNCTION

1975 ◽  
Vol 78 (1) ◽  
pp. 86-90 ◽  
Author(s):  
P. Sederberg-Olsen ◽  
C. Binder ◽  
H. Kehlet
Keyword(s):  
Renal Function ◽  
Urinary Excretion ◽  
Impaired Renal Function ◽  
Filtration Rate ◽  
Urinary Cortisol ◽  
Dose Rates ◽  
Total Cortisol ◽  
Free Cortisol ◽  
Free Urinary Cortisol

ABSTRACT Total cortisol and free, non protein-bound cortisol in plasma and urinary excretion of unconjugated free cortisol were measured during iv infusion of cortisol at varying dose rates in eight patients with impaired renal function. The results showed that free urinary cortisol decreased with decreased glomerular filtration rate (GFR), also compared to free cortisol level in plasma. An increase in free cortisol in plasma had no influence on GFR. It is concluded that determination of free urinary cortisol, otherwise useful in diagnosing Cushing's syndrome, may be of less value in patients with impaired renal function.

scholarly journals Convenient chromatographic prepurification step before measurement of urinary cortisol by radioimmunoassay

1997 ◽  
Vol 43 (5) ◽  
pp. 786-793 ◽  
Author(s):  
Gilles Morineau ◽  
James Gosling ◽  
Marie-Claude Patricot ◽  
Hany Soliman ◽  
Philippe Boudou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Isotope Dilution ◽  
Hplc Method ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Purification Step ◽  
Urinary Cortisol ◽  
Chromatography Mass Spectrometry ◽  
Free Urinary Cortisol

Abstract We applied various prepurification protocols (extraction with different solvents, liquid/solid separation on bonded silica media, Celite, and Sephadex LH20 chromatography) with a range of commercially available RIA kits to measure cortisol in urine samples. We then compared the results with the concentrations measured by a HPLC method validated with reference to isotope dilution gas chromatography–mass spectrometry. We conclude that chromatography on a commercial, prepacked diol minicolumn (Waters™ Sep-Pak Vac RC) in combination with dichloromethane extraction is a convenient and very effective purification step before RIA of urinary cortisol in patients not receiving corticoid medication. We tested numerous steroids for interference and found that free polar cortisol derivatives (hydroxylated or hydrogenated) could only partially account for the overestimations routinely encountered when free urinary cortisol concentrations are measured by direct RIA.

FREE URINARY CORTISOL AS A REPRESENTATIVE STEROID OF THE URINARY PORTER-SILBER-CHROMOGENS

1972 ◽  
Vol 70 (1) ◽  
pp. 65-72 ◽  
Author(s):  
H. Stolecke
Keyword(s):  
Plasma Cortisol ◽  
Biologically Active ◽  
Urinary Cortisol ◽  
Pathological Conditions ◽  
Urinary Steroids ◽  
Free Urinary Cortisol ◽  
Loading Tests ◽  
The Mean ◽  
Cortisol Metabolites

ABSTRACT In this investigation an attempt was made to find a compound based on urinary steroids which characterise the mean cortisol metabolites. Free urinary cortisol turned out to be useful for this purpose. Thus a positive correlation can be demonstrated under basal conditions as well as after standardized loading tests with metyrapone and β1-24-tetracosactide2). With regard to the lens-effect described in a previous paper and the mechanisms leading to this effect it was considered whether the determination of free urinary cortisol could be used as a sensitive estimation of enhanced adrenal activity, providing this rise in activity led to an increase of the biologically active plasma cortisol fraction. Further studies of free urinary cortisol in pathological conditions other than Cushing's syndrome as well as calculations of ratios of urinary C-17-OHCS and plasma cortisol are necessary, however, in order to evaluate the diagnostic possibilities in use of free urinary cortisol as a parameter.

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