FREE URINARY CORTISOL AS A REPRESENTATIVE STEROID OF THE URINARY PORTER-SILBER-CHROMOGENS

1972 ◽  
Vol 70 (1) ◽  
pp. 65-72 ◽  
Author(s):  
H. Stolecke
Keyword(s):  
Plasma Cortisol ◽  
Biologically Active ◽  
Urinary Cortisol ◽  
Pathological Conditions ◽  
Urinary Steroids ◽  
Free Urinary Cortisol ◽  
Loading Tests ◽  
The Mean ◽  
Cortisol Metabolites

ABSTRACT In this investigation an attempt was made to find a compound based on urinary steroids which characterise the mean cortisol metabolites. Free urinary cortisol turned out to be useful for this purpose. Thus a positive correlation can be demonstrated under basal conditions as well as after standardized loading tests with metyrapone and β1-24-tetracosactide2). With regard to the lens-effect described in a previous paper and the mechanisms leading to this effect it was considered whether the determination of free urinary cortisol could be used as a sensitive estimation of enhanced adrenal activity, providing this rise in activity led to an increase of the biologically active plasma cortisol fraction. Further studies of free urinary cortisol in pathological conditions other than Cushing's syndrome as well as calculations of ratios of urinary C-17-OHCS and plasma cortisol are necessary, however, in order to evaluate the diagnostic possibilities in use of free urinary cortisol as a parameter.

URINARY EXCRETION OF FREE CORTISOL IN IMPAIRED RENAL FUNCTION

1975 ◽  
Vol 78 (1) ◽  
pp. 86-90 ◽  
Author(s):  
P. Sederberg-Olsen ◽  
C. Binder ◽  
H. Kehlet
Keyword(s):  
Renal Function ◽  
Urinary Excretion ◽  
Impaired Renal Function ◽  
Filtration Rate ◽  
Urinary Cortisol ◽  
Dose Rates ◽  
Total Cortisol ◽  
Free Cortisol ◽  
Free Urinary Cortisol

ABSTRACT Total cortisol and free, non protein-bound cortisol in plasma and urinary excretion of unconjugated free cortisol were measured during iv infusion of cortisol at varying dose rates in eight patients with impaired renal function. The results showed that free urinary cortisol decreased with decreased glomerular filtration rate (GFR), also compared to free cortisol level in plasma. An increase in free cortisol in plasma had no influence on GFR. It is concluded that determination of free urinary cortisol, otherwise useful in diagnosing Cushing's syndrome, may be of less value in patients with impaired renal function.

Characterization of 10 Biogenic Amines in Male Silkworm Moth by HPLC with Precolumn Derivatization

2020 ◽  
Vol 16 (5) ◽  
pp. 608-614
Author(s):  
Mingqin Fan ◽  
Yun Ai ◽  
Wenjie Zhao ◽  
Yanni Sun ◽  
Jianli Liu ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Biologically Active ◽  
Low Molecular Weight ◽  
Precolumn Derivatization ◽  
Mean Values ◽  
The Mean ◽  
Toxic Potential ◽  
First Time

Background: Biogenic Amines (BAs) are biologically active nitrogenous organic compounds of low molecular weight, which are frequently found in a wide variety of foods, beverages and herbs due to their toxic potential in humans. Male Silkworm Moth (MSM), a Traditional Chinese Medicine (TCM), has been exploited and utilized as nutritious liquor based on its traditional effects in the Chinese community. Objective: The objective of this study was to develop an HPLC with Dns-Cl derivatization method for characterizing overall BAs in MSM and providing data for further evaluating its activities and safety profiles. Methods: The method has acceptable sensitivity, precision, accuracy, selectivity and recovery, and was successfully applied to the determination of the BAs contents in MSM for the first time. Results: In the analysis of 10 batches of MSM samples, serotonin and dopamine were not found in detectable concentrations in any samples, and the most abundant amine found was putrescine. The mean values of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine determined in the samples were found to be 34.7 mg/ kg, 16.1 mg/ kg, 218.3 mg/ kg, 37.9 mg/ kg, 12.1 mg/ kg, 18.2 mg/ kg, 4.5mg/ kg, and 0.9 mg/ kg, respectively. Conclusion: The contents of BAs in 10 batches of MSM were below the maximum recommended limits, and MSM can be used safely.

scholarly journals Homogeneous, Nonradioactive, Enzymatic Assay for Plasma Pyridoxal 5-Phosphate

2002 ◽  
Vol 48 (9) ◽  
pp. 1560-1564 ◽  
Author(s):  
Qinghong Han ◽  
Mingxu Xu ◽  
Li Tang ◽  
Xuezhong Tan ◽  
Xiuying Tan ◽  
...  
Keyword(s):  
Vitamin B6 ◽  
Independent Risk Factor ◽  
Limit Of Detection ◽  
Active Form ◽  
Enzymatic Assay ◽  
Biologically Active ◽  
Radioenzymatic Assay ◽  
The Mean ◽  
American Laboratory

Abstract Background: Pyridoxal 5′-phosphate (PLP) is the biologically active form of vitamin B6. Clinical studies suggest that low PLP concentrations are an independent risk factor for cardiovascular and other diseases. However, PLP concentrations are not routinely diagnosed because of the lack of a homogeneous, nonradioactive assay. We describe a homogeneous, nonradioactive, enzymatic PLP assay that uses the apo form of the PLP-dependent recombinant enzyme, homocysteine-α,γ-lyase (rHCYase). Methods: PLP was removed from holoenzyme rHCYase by incubation with hydroxylamine to obtain apo-rHCYase. The restoration of enzymatic activity by reconstitution of the holoenzyme was linearly related to the amount of PLP bound to the enzyme. The amplification principle of the assay allowed nanomolar concentrations of PLP to be measured by the conversion (by reconstituted holo-rHCYase) of millimolar concentrations of homocysteine to H2S. N,N-Dibutylphenylenediamine (DBPDA) was used for determination of H2S, the combination of which forms a chromophore with high absorbance. The assay was initiated by incubation of 5 μL of plasma with apo-rHCYase in a binding buffer for 60 min at 37 °C. Homocysteine (2.5 mmol/L) was added to the assay buffer and incubated at 37 °C for 20 min. The DBPDA reaction was allowed to progress for 10 min and then read at 675 nm. Results: The PLP enzymatic assay has a lower limit of detection of 5 nmol/L and is linear to 200 nmol/L. The recovery of PLP was 98%. The mean within- and between-run CVs were 9.6% and 12%, respectively. Correlation of 45 samples in the PLP enzymatic assay and the B63H radioenzymatic assay (American Laboratory Products Co., Ltd.) yielded: y = 0.9367x + 10.569 (R2 = 0.9201). Conclusions: This new PLP assay is the first homogeneous, nonradioactive, vitamin B6 diagnostic method. The assay is applicable to chemistry automated analyzers and may have wide clinical use.

Effect of cyproterone acetate on glucocorticoid secretion in patients treated for hirsutism

1983 ◽  
Vol 104 (2) ◽  
pp. 222-226 ◽  
Author(s):  
I. M. Holdaway ◽  
M. S. Croxson ◽  
M. C. Evans ◽  
J. France ◽  
A. Sheehan ◽  
...  
Keyword(s):  
Cyproterone Acetate ◽  
Plasma Cortisol ◽  
Slight Reduction ◽  
Cortisol Response ◽  
Urinary Cortisol ◽  
Reverse Sequence ◽  
Urinary Cortisol Excretion ◽  
The Mean ◽  
Pre Treatment ◽  
Adrenal Glucocorticoid

Abstract. The effect of cyproterone acetate (CA) on adrenal glucocorticoid secretion was studied in 35 women with hirsutism. Patients were treated for 9 months with 100 mg CA orally 10 days each month, administered in reverse sequence with 21 days of a combination oral contraceptive containing 50 μg ethinyloestradiol and 2 mg CA. During treatment one patient had a mildly impaired plasma cortisol response to insulin-induced hypoglycaemia and 2 patients showed slight reduction of the plasma cortisol response to ACTH. Responses to metyrapone were normal in all patients tested. Overall, the mean response to these tests was significantly greater during CA treatment compared with pre-treatment measurements, probably due to effects of oestrogen on cortisol-binding globulin. In all patients urinary cortisol excretion remained normal and no patient demonstrated any features of steroid insufficiency. Thus it appears that CA has no untoward effect upon glucocorticoid secretion when given in high dosage for prolonged periods to hirsute women.

Cortisol production rate determined by radio gas-chromatography.

1977 ◽  
Vol 23 (3) ◽  
pp. 518-521 ◽  
Author(s):  
H J Derks ◽  
F A Muskiet ◽  
B G Wolthers ◽  
J H Thijssen ◽  
N M Drayer
Keyword(s):  
Production Rate ◽  
Chromatographic Method ◽  
Correlation Coefficients ◽  
Urinary Cortisol ◽  
Coefficients Of Variation ◽  
Specific Activities ◽  
Gas Chromatographic Method ◽  
Layer Chromatography ◽  
Cortisol Metabolites

Abstract We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducible, the coefficients of variation ranging from 8-15% of allotetrahydrocortisol/tetrahydrocortisol and from 9-19% for tetrahydrocortisone. Correlation coefficients were 0.966 and 0.998 for tetrahydrocortisone and allotetrahydrocortisol/tetrahydrocortisol, respectively, when the method was compared with a method involving thin-layer chromatography and colorimetry. Only one chromatographic step is needed for both purification and quantitation, thus time and effort are saved.

Determination of Urinary Cortisol Metabolites in Spot Urine of Patients with Uterine Cancer Utilizing Segmental GC/MS/MS

2011 ◽  
pp. P2-220-P2-220
Author(s):  
Vijaya L Nacharaju ◽  
Yi-Chun Lee ◽  
Ghadir Salame ◽  
Tana Shah ◽  
Erin Stevens ◽  
...  
Keyword(s):  
Uterine Cancer ◽  
Urinary Cortisol ◽  
Spot Urine ◽  
Cortisol Metabolites

scholarly journals PERBANDINGAN PENENTUAN KADAR TIROKSIN ENZYME LINKED IMMUNOFLUORESCENT ASSAY (ELFA) DAN ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA)

2016 ◽  
Vol 18 (1) ◽  
pp. 15
Author(s):  
Faizah Yunianti ◽  
Siswanto Darmadi ◽  
M Y. Probohoesodo ◽  
Budiono Budiono
Keyword(s):  
Clinical Pathology ◽  
Special Treatment ◽  
Lower Yield ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Immunosorbant Assay ◽  
Pathological Conditions ◽  
The Mean ◽  
Enzyme Linked Immunosorbant Assay

The determination of thyroxin (T4) is known as a good indicator to know the condition of thyroid function. In the hyperthyroid state, are shown clearly the increased levels of T4, while in the hypothyroid state their levels always decrease. The T4 levels change due to the physiological and pathological conditions on the ability of thyroxin binding globulin (TBG). The T4 measurement can be performed using an enzyme-linked fluorescent immunoassay (ELFA) or enzyme-linked immunosorbant assay (ELISA). Both ELFA and ELISA can detect T4 antigen. In these research fifty one randomized samples sera from the Clinical Pathology laboratory, at Dr. Soetomo Hospital, consisting of 11 males and 40 females, were studied. They were comparing using ELFA and ELISA to know the levels of T4. In this study the method used is a cross sectional observational, and the statistical analysis with nonparametric Spearman Correlation Rank’s test and Wilcoxon Signed Rank’s Test. In the results by using ELFA were found the mean and SD values 8.78 μg/dL/5.39 μg/dL, whereas by ELISA 11.06 μg/dL/5.38 μg/dL. These results showed a correlation between ELFA and ELISA with rs 0.576. The T4 levels results showed as follows, hypothyroid ELFA 8 (15.7%), ELISA 6 (11.8%); normal levels ELFA 32 (62.7%), ELISA 29 (56.9%); hyperthyroid ELFA 11 (21.6%), ELISA 16 (31.4%) with p 0.090 which was not considered significant. It can be concluded from the showen results that in the determination of T4 concentration using ELFA gave a lower yield compared with ELISA. Further examination is required by special treatment of the sample or other way to find out which one is the best way for determination of T4 levels.

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