A multicomponent method for Fusarium toxins in cereal based food and feed samples using HPLC-MS/MS

2005 ◽  
Vol 21 (1) ◽  
pp. 18-22 ◽  
Author(s):  
S. Biselli ◽  
H. Wegner ◽  
C. Hummert
Keyword(s):  
Fusarium Toxins ◽  
Food And Feed ◽  
Feed Samples

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

Monitoring of polychlorinated dibenzo-p-dioxins and dibenzofurans, dioxin-like PCBs and polycyclic aromatic hydrocarbons in food and feed samples from Ismailia city, Egypt

Chemosphere ◽  
2007 ◽  
Vol 66 (10) ◽  
pp. 1962-1970 ◽  
Author(s):  
N. Loutfy ◽  
M. Fuerhacker ◽  
P. Tundo ◽  
S. Raccanelli ◽  
M. Tawfic Ahmed
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic ◽  
Food And Feed ◽  
Feed Samples

scholarly journals Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS

2017 ◽  
Vol 239 ◽  
pp. 1087-1097 ◽  
Author(s):  
Katrina Campbell ◽  
Ana L. Ferreira Cavalcante ◽  
Pamela Galvin-King ◽  
Michalina Oplatowska-Stachowiak ◽  
Catherine Brabet ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Food And Feed ◽  
Feed Samples

Isolation, enumeration and PCR characterization of aflatoxigenic fungi from food and feed samples in India

2004 ◽  
Vol 21 (6) ◽  
pp. 809-813 ◽  
Author(s):  
D Somashekar ◽  
E.R Rati ◽  
S Anand ◽  
A Chandrashekar
Keyword(s):  
Aflatoxigenic Fungi ◽  
Food And Feed ◽  
Feed Samples

Pattern and distribution of ergot alkaloids in cereals and cereal products from European countries

2014 ◽  
Vol 7 (2) ◽  
pp. 217-230 ◽  
Author(s):  
S.V. Malysheva ◽  
D.A. Larionova ◽  
J. Diana Di Mavungu ◽  
S. De Saeger
Keyword(s):  
Ergot Alkaloids ◽  
Food Samples ◽  
Human Consumption ◽  
Limit Of Quantification ◽  
Cereal Products ◽  
Liquid Chromatography Tandem Mass ◽  
Food And Feed ◽  
Animal Feeding ◽  
Sample Set ◽  
Feed Samples

This paper reports on the occurrence of ergot alkaloids in cereals and cereal products in Europe. It includes occurrence data our group previously submitted to the European Food Safety Authority and new data we gathered afterwards. A total of 1,065 samples of cereals and cereal products intended for human consumption and animal feeding were analysed by liquid chromatography-tandem mass spectrometry for the presence of ergot alkaloids. The sample set included rye-, wheat- and multigrain-based food as well as rye-, wheat- and triticale-based feed. The study revealed that 59% of the analysed food and feed samples were contaminated with ergot alkaloids to some extent. In 55% of the samples, the levels of the -ine isomers were above the limit of quantification (LOQ), while contamination with the -inine isomers was found in 51% of the samples. The median values for the main ergot alkaloids (-ine forms) and the epimers (-inine forms) were 1 and 2 μg/kg, respectively. Ergot alkaloids were present in 84% of rye food, 67% of wheat food, 48% of multigrain food, 52% of rye feed, 27% of wheat feed, and 44% of triticale feed at total alkaloid levels ranging from ≤1 (LOQ) to 12,340 μg/kg. Though the highest frequencies of contamination were observed for food samples, the feed samples, in particular Swiss rye feed, accounted for the highest levels of ergot alkaloids. The frequencies and levels of contamination were significantly lower in organic samples compared to conventional samples. Maximum levels of individual ergot alkaloids up to 3,270 μg/kg (for ergotamine) were observed. Overall, ergosine, ergokryptine and ergocristine were the frequently occurring ergot alkaloids. The co-occurrence of all six ergot alkaloids was noted in 35% of the positive samples. Occurrence of a single ergot alkaloid was mainly observed for ergometrine.

Current Status of Immunofluorescent Methodology for Salmonellae1

1981 ◽  
Vol 44 (5) ◽  
pp. 381-384 ◽  
Author(s):  
BERENICE M. THOMASON
Keyword(s):  
Fluorescent Antibody ◽  
Screening Method ◽  
Rapid Screening ◽  
Current Status ◽  
Official Method ◽  
Pathogenic Microorganisms ◽  
Advantages And Disadvantages ◽  
Food And Feed ◽  
History Of ◽  
Feed Samples

The fluorescent antibody (FA) method for detecting salmonellae in food and feed samples is now an official method of the Association of Official Analytical Chemists. The need for a rapid screening method for detecting these pathogenic microorganisms in suspect products led to development of the FA method. A brief history of the development of the FA test, its evaluation by various investigators and its use by food laboratories are described in this report. Advantages and disadvantages of the method are discussed with suggestions for improvement of the technique.

Meeting the European Commission performance criteria for the use of triple quadrupole GC-MS/MS as a confirmatory method for PCDD/Fs and dl-PCBs in food and feed samples

2016 ◽  
Vol 408 (13) ◽  
pp. 3511-3525 ◽  
Author(s):  
Manuela Ábalos ◽  
Cristian I. Cojocariu ◽  
Paul Silcock ◽  
Dominic Roberts ◽  
Diana M. Pemberthy ◽  
...  
Keyword(s):  
European Commission ◽  
Performance Criteria ◽  
Triple Quadrupole ◽  
Food And Feed ◽  
Feed Samples

Liquid Chromatographic Determination of Fumonisins B1, B2, and B3 in Foods and Feeds

1992 ◽  
Vol 75 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Eric W Sydenham, ◽  
Gordon S Shephard, ◽  
Peter G Thiel
Keyword(s):  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Natural Occurrence ◽  
Liquid Chromatographic Method ◽  
Food And Feed ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Feed Samples ◽  
Initial Extraction

Abstract Three recently described and toxlcologlcally Important Fusarlum mycotoxins, fumonisln B1 (FB1), fumonisln B2 (FB2), and fumonisln B3 (FB3), are the major fumonisins produced In cultures of F. monlllforme, a fungus that occurs worldwide on corn. Contamination of food and feed with F. monlllforme has been associated with a number of diseases In both animals and humans. Aspects of a recently reported liquid chromatographic method for the determination of FB1 and FB2 in corn, including Initial extraction, extract purification, and stability of derivatives, were Investigated and, where necessary, optimized further both to reduce the analysis time and to include the co-determlnatlon of FB3. The method was applied for the determination of FB3, in a series of U.S. feed samples associated with outbreaks of equine leukoencephalomalacla, which were shown previously to contain both FBi and FB2. Twelve of the 13 feed samples contained FB3 at levels ranging between 50 and 2650 ng/g, corresponding to 2.2-18% of the total fumonisln concentrations present in the FB3-posltlve feed samples. This is the first report of the natural occurrence of FB3.

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