Expression profiling of lipopolysaccharide target genes in RAW264.7 cells by oligonucleotide microarray analyses

2006 ◽  
Vol 29 (10) ◽  
pp. 890-897 ◽  
Author(s):  
Hao Huang ◽  
Cheol Kyu Park ◽  
Ji Yoon Ryu ◽  
Eun-Ju Chang ◽  
Youngkyun Lee ◽  
...  
2014 ◽  
Vol 2014 ◽  
pp. 1-3
Author(s):  
Ichiro Akagi ◽  
Osamu Ishibashi ◽  
Takeshi Matsutani ◽  
Nobutoshi Hagiwara ◽  
Akihisa Matsuda ◽  
...  

Despite the undisputed importance of altered microRNA (miRNA) expression in various cancers, there is limited information on the clinicopathologic significance of cancer-related miRNAs in esophageal squamous cell carcinoma (ESCC). Previously, it was reported that the expression of several miRNAs was dysregulated in ESCC. However, the target genes of these miRNAs have not been identified. Furthermore, additional miRNAs in humans have been discovered recently, indicating that revised miRNA and gene expression profiling for ESCC are necessary. Here, we provide datasets from microarray analyses to identify miRNA and mRNA expression comprehensively in Het-1A, a normal human esophageal squamous cell line, and three human ESCC cell lines.


2002 ◽  
Vol 48 (11) ◽  
pp. 1873-1882 ◽  
Author(s):  
Elaine M Weidenhammer ◽  
Brenda F Kahl ◽  
Ling Wang ◽  
Larry Wang ◽  
Melanie Duhon ◽  
...  

Abstract Background: Electronic microarrays comprise independent microelectrode test sites that can be electronically biased positive or negative, or left neutral, to move and concentrate charged molecules such as DNA and RNA to one or more test sites. We developed a protocol for multiplexed gene expression profiling of mRNA targets that uses electronic field-facilitated hybridization on electronic microarrays. Methods: A multiplexed, T7 RNA polymerase-mediated amplification method was used for expression profiling of target mRNAs from total cellular RNA; targets were detected by hybridization to sequence-specific capture oligonucleotides on electronic microarrays. Activation of individual test sites on the electronic microarray was used to target hybridization to designated subsets of sites and allow comparisons of target concentrations in different samples. We used multiplexed amplification and electronic field-facilitated hybridization to analyze expression of a model set of 10 target genes in the U937 cell line during lipopolysaccharide-mediated differentiation. Performance of multiple genetic analyses (single-nucleotide polymorphism detection, gene expression profiling, and splicing isoform detection) on a single electronic microarray was demonstrated using the ApoE and ApoER2 genes as a model system. Results: Targets were detected after a 2-min hybridization reaction. With noncomplementary capture probes, no signal was detectable. Twofold changes in target concentration were detectable throughout the (∼64-fold) range of concentrations tested. Levels of 10 targets were analyzed side by side across seven time points. By confining electronic activation to subsets of test sites, polymorphism detection, expression profiling, and splicing isoform analysis were performed on a single electronic microarray. Conclusions: Microelectronic array technology provides specific target detection and quantification with advantages over currently available methodologies for targeted gene expression profiling and combinatorial genomics testing.


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