Preparation for protein separation of an ion-exchange polymeric stationary phase presenting amino acid and amine units through surface graft polymerization

2005 ◽  
Vol 13 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Seong-Ho Choi ◽  
Kwang-Pill Lee ◽  
Chang-Ho Shin
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Stationary Phase ◽  
Protein Separation ◽  
Graft Polymerization ◽  
Surface Graft ◽  
Polymeric Stationary Phase

scholarly journals 6S RNA regulation of relA alters ppGpp levels in early stationary phase

Microbiology ◽  
2010 ◽  
Vol 156 (12) ◽  
pp. 3791-3800 ◽  
Author(s):  
Amy T. Cavanagh ◽  
Pete Chandrangsu ◽  
Karen M. Wassarman
Keyword(s):  
Amino Acid ◽  
Stationary Phase ◽  
Regulation Of Transcription ◽  
Early Stationary Phase ◽  
Rna Regulation ◽  
Global Regulators ◽  
Expression Of Genes ◽  
Non Coding Rna ◽  
6S Rna ◽  
Altered Regulation

6S RNA is a small, non-coding RNA that interacts directly with σ 70-RNA polymerase and regulates transcription at many σ 70-dependent promoters. Here, we demonstrate that 6S RNA regulates transcription of relA, which encodes a ppGpp synthase. The 6S RNA-dependent regulation of relA expression results in increased ppGpp levels during early stationary phase in cells lacking 6S RNA. These changes in ppGpp levels, although modest, are sufficient to result in altered regulation of transcription from σ 70-dependent promoters sensitive to ppGpp, including those promoting expression of genes involved in amino acid biosynthesis and rRNA. These data place 6S RNA as another player in maintaining appropriate gene expression as cells transition into stationary phase. Independent of this ppGpp-mediated 6S RNA-dependent regulation, we also demonstrate that in later stationary phase, 6S RNA continues to downregulate transcription in general, and specifically at a subset of the amino acid promoters, but through a mechanism that is independent of ppGpp and which we hypothesize is through direct regulation. In addition, 6S RNA-dependent regulation of σ S activity is not mediated through observed changes in ppGpp levels. We suggest a role for 6S RNA in modulating transcription of several global regulators directly, including relA, to downregulate expression of key pathways in response to changing environmental conditions.

Preparation of a novel dual-function strong cation exchange/hydrophobic interaction chromatography stationary phase for protein separation

Talanta ◽  
2012 ◽  
Vol 98 ◽  
pp. 86-94 ◽  
Author(s):  
Kailou Zhao ◽  
Li Yang ◽  
Xuejiao Wang ◽  
Quan Bai ◽  
Fan Yang ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Cation Exchange ◽  
Hydrophobic Interaction ◽  
Protein Separation ◽  
Hydrophobic Interaction Chromatography ◽  
Dual Function ◽  
Strong Cation Exchange

Characteristics of a novel stationary phase using protein separation as an application

1989 ◽  
Vol 26 (11) ◽  
pp. 390 ◽  
Author(s):  
M. A. Fung ◽  
A. F. Drake ◽  
C. F. Simpson ◽  
D. P. Ndiomu ◽  
S. O. Akapo ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Protein Separation

The separation and amino acid analysis of collagen crosslinks on an extended basic ion-exchange column

1981 ◽  
Vol 114 (2) ◽  
pp. 310-315 ◽  
Author(s):  
Timothy J. Housley ◽  
Marvin L. Tanzer
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Analysis ◽  
Collagen Crosslinks ◽  
Exchange Column ◽  
Ion Exchange Column

Ion exchange fabric synthesized by graft polymerization and its application to ultra-pure water production

2010 ◽  
Vol 79 (3) ◽  
pp. 223-226 ◽  
Author(s):  
T. Takeda ◽  
M. Tamada ◽  
N. Seko ◽  
Y. Ueki
Keyword(s):  
Ion Exchange ◽  
Graft Polymerization ◽  
Pure Water ◽  
Water Production ◽  
Ultra Pure Water

scholarly journals AMINO ACID COMPOSITION AND ELECTROPHORETIC PROPERTIES OF HYPERTENSIN I

1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Point ◽  
Column Chromatography ◽  
Isoleucine Leucine ◽  
Ion Exchange Column

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.

scholarly journals Surface Graft Polymerization of Styrene onto Nano-Sized Silica with a One-Pot Method

2003 ◽  
Vol 35 (4) ◽  
pp. 379-383 ◽  
Author(s):  
Peng Liu ◽  
Jun Tian ◽  
Weimin Liu ◽  
Qunji Xue
Keyword(s):  
Graft Polymerization ◽  
One Pot ◽  
Surface Graft

Effect of hydroxyapatite whisker surface graft polymerization on water sorption, solubility and bioactivity of the dental resin composite

2015 ◽  
Vol 53 ◽  
pp. 150-155 ◽  
Author(s):  
Fengwei Liu ◽  
Xiaoze Jiang ◽  
Shuang Bao ◽  
Ruili Wang ◽  
Bin Sun ◽  
...  
Keyword(s):  
Water Sorption ◽  
Graft Polymerization ◽  
Resin Composite ◽  
Dental Resin ◽  
Surface Graft
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