PIPERACILLIN: A NEW MONOSODIUM, SEMISYNTHETIC PENICILLIN

Regarding the article on Pencillin in Pediatrics in Review. 1995;16:83-90 (March), a reader expressed concern about the answers to the first and second questions in the PIR Quiz. In the first question, the clinical picture presented was clearly meningococcemia, and aqueous penicillin G IV is the drug of choice. However, as Dr. Feigin states, "Given the choices in question #2, we would probably choose methicillin because the question suggests that Staphylococcus aureus may be the offending organism. Of the various semisynthetic penicillin derivatives listed, methicillin is the one with the lowest degree of protein binding (20%) and, thus, is least likely to displace bilirubin from albumin binding sites. Therefore, its use in the newborn period may be indicated."

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Kloning Gen pcbC dari Penicillium chrysogenum ke dalam Plasmid pPICZA untuk Pengembangan Produksi Penisilin G

Bioma Berkala Ilmiah Biologi ◽

10.14710/bioma.16.1.33-38 ◽

2014 ◽

Vol 16 (1) ◽

pp. 33

Author(s):

Risma Wiharyani ◽

Dudi Hardianto ◽

Hermin Pancasakti Kusumaningrum ◽

Anto Budiharjo

Keyword(s):

Dna Sequences ◽

Penicillium Chrysogenum ◽

Raw Materials ◽

Raw Material ◽

Penicillin G ◽

Gene Fragment ◽

Semisynthetic Penicillin ◽

Plasmid Amplification ◽

Blast Program ◽

Important Enzyme

Availability of drugs in Indonesia is still limited by the high prices of drugs due to on the imported raw materials that reaches 95%. Developing antibiotic raw materials can be achieved by increasing of penicillin G production, which is the raw material for the formation of semisynthetic penicillin derivatives through the production of 6-aminopenisillanic acid (6-APA). One of the important enzyme in the penicillin G biosynthesis is Isopenisilin N Synthase (IPNS) that encodes by pcbC gene on Penicillium chrysogenum. This study aimed to obtain a recombinant of pcbC gene fragments that is inserted into pPICZA plasmid. Amplification of pcbC gene used pcbC-F and pcbC-R primers. The pcbC gene fragment was inserted into pPICZA vector and then transformed into TOP 10 F’. The results showed that the recombinant of the pcbC gene fragment from P. chrysogenum has been obtained. Analysis of DNA sequences using the BLAST program showed that the pcbC gene fragment has high homology (99%) with the pcbC gene from P. chrysogenum Wisconsin 54-1255 and P. chrysogenum AS-P-78 which encodes IPNS Keywords: pcbC Gene, Penicillium chrysogenum, cloning, penicillin G

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Ticarcillin: Pharmacokinetics in Man According to Different Administration Schedules

Journal of International Medical Research ◽

10.1177/030006057700500504 ◽

1977 ◽

Vol 5 (5) ◽

pp. 308-312 ◽

Cited By ~ 5

Author(s):

A Dalhoff ◽

D Höffler

Keyword(s):

Steady State ◽

Total Dose ◽

Continuous Infusion ◽

Intravenous Infusion ◽

Half Life ◽

Loading Dose ◽

Serum Concentrations ◽

Semisynthetic Penicillin ◽

Steady State Serum ◽

Administration Schedules

The pharmacokinetic characteristics of ticarcillin, a semisynthetic penicillin more active than carbenicillin against Pseudomonas, were studied. Following a rapid intravenous infusion of 1 g, 2 g, 5 g and 10 g ticarcillin respectively the serum half-life was 72·4 minutes independent of the dosage administered. If ticarcillin is administered under steady-state conditions, e.g. continuous infusion of either 2 g/hr or 1 g/hr following a loading dose of 1 g (total dose 5 g) the average steady-state serum concentrations are 125 μg/ml and 105 μg/ml respectively.

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