Diagnosis of latex allergy: Comparison of histamine release and flow cytometric analysis of basophil activation

1996 ◽  
Vol 45 (S1) ◽  
pp. S35-S36 ◽  
Author(s):  
J. Sainte-Laudy ◽  
C. Vallon ◽  
J. C. Guerin
Keyword(s):  
Histamine Release ◽  
Flow Cytometric Analysis ◽  
Basophil Activation ◽  
Latex Allergy ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of drug-Induced basophil histamine release

2015 ◽  
Vol 90 (3) ◽  
pp. 285-288 ◽  
Author(s):  
N. Cop ◽  
A. P. Uyttebroek ◽  
V. Sabato ◽  
C. H. Bridts ◽  
L. S. De Clerck ◽  
...  
Keyword(s):  
Histamine Release ◽  
Flow Cytometric Analysis ◽  
Drug Induced ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of the inhibition of human basophil activation by histamine high dilutions - a replication study

2021 ◽  
Vol 11 (40) ◽  
pp. 172-173
Author(s):  
Chantal Wälchli ◽  
Stephan Baumgartner
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometric Analysis ◽  
Systematic Errors ◽  
Basophil Activation ◽  
Human Basophil ◽  
Specific Marker ◽  
Flow Cytometric ◽  
High Dilutions ◽  
Independent Replication ◽  
Human Basophils

Background: Inhibition of human basophil activation by highly diluted histamine was reported to be a reliable experimental model to examine biological effects of high dilutions. However, independent replications did not always yield concordant results. Aims: We aimed at performing an independent replication of a former study [1] using rigorously controlled experimental conditions to minimise confounding factors. Materials and Methods: In 20 independent experiments, human basophils were treated with highly diluted histamine (15cH, 16cH, corresponding to 10-30-10-32 M) prior to activation by fMLP (formyl-methionyl-leucyl-phenylalanine peptide). Controls were treated with analogously diluted water (15cH, 16cH). The dilutions were prepared freshly for each experiment in deionised water by successive steps of centesimal dilution and agitation (10 s vortex at high speed). Highly diluted samples were blinded and randomised. All samples were set in triplicates. Activated basophils were determined by flow cytometry using anti-CD203c. 20 independent systematic negative control (SNC) experiments were carried out to investigate possible systematic errors. Results: No difference in basophil activation was observed between the highly diluted histamine samples and the highly diluted water controls. There was no evidence for a blood donor specificity of the results. The SNC experiments demonstrated the stability of the test system. Experimental variability within and between experiments was slightly reduced for the highly diluted histamine samples. Discussion: This study was designed as an independent reproduction of a former study [1]. Though we strictly adopted the experimental procedure described in [1], our results do not confirm the large inhibitory effects observed for histamine 15cH and 16cH. This lack of reproducibility might be due to minor differences in the experimental design, such as blinding and randomising of the samples, which we chose to perform in order to reduce the possibility of artifacts but was omitted in the former study. Conclusions: Laboratory independent replication of homeopathic basic research experiments is still a challenge. Assuming that the results formerly obtained with this model were not due to systematic errors, the quest identifying the crucial factors for successful reproducibility is open for future research. Keywords: Human basophils; histamine; high dilutions; flow cytometry Reference: [1] Sainte-Laudy J, Belon P. Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c. Homeopathy. 2006;95:3-8.

FLOW CYTOMETRIC BASOPHIL ACTIVATION TEST: DETECTION OF CD63 EXPRESSION AS A USEFUL AID TO DIAGNOSIS OF LATEX ALLERGY

2006 ◽  
Vol 97 (5) ◽  
pp. 715-716 ◽  
Author(s):  
Eustachio Nettis ◽  
Maria C. Colanardi ◽  
Porzia Pia Dambra ◽  
Laura Capuzzimati ◽  
Maria Paola Loria ◽  
...  
Keyword(s):  
Basophil Activation Test ◽  
Basophil Activation ◽  
Latex Allergy ◽  
Activation Test ◽  
Flow Cytometric ◽  
Cd63 Expression

Apoptotic Effects of Melittin on 4T1 Breast Cancer Cell Line is associated with Up Regulation of Mfn1 and Drp1 mRNA Expression

2020 ◽  
Vol 20 (7) ◽  
pp. 790-799 ◽  
Author(s):  
Farnaz D. Moghaddam ◽  
Pejman Mortazavi ◽  
Somayeh Hamedi ◽  
Mohammad Nabiuni ◽  
Nasim H. Roodbari
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Hemolytic Activity ◽  
Breast Cancer Cells ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Flow Cytometric ◽  
4T1 Cells ◽  
4T1 Breast Cancer ◽  
Apoptotic Effects

Background and Purpose: Melittin, as the main ingredient of honeybee venom, that has shown anticancer properties. The present study aimed at investigating the cytotoxic impacts of melittin on 4T1 breast cancer cells. Methods: Hemolytic activity of different concentrations (0.125, 0.25, 0.5, 1, 2, 4, 8μg/ml) of melittin was assayed and then cytotoxicity of selected concentrations of melittin (2, 4, 8, 16, 32, and 64μg/ml), 2 and 4μg/ml of cisplatin and 0.513, 0.295 and 0.123μg/ml of doxorubicin was evaluated on 4T1 cells using MTT assay. We used Morphological evaluation and flow cytometric analysis was used. Real time PCR was also used to determine mRNA expression of Mfn1 and Drp1 genes. Results: All compounds showed anti-proliferative effects on the tumor cell line with different potencies. Melittin had higher cytotoxicity against 4T1 breast cancer cells (IC50= 32μg/ml-72h) and higher hemolytic activity (HD50= 1μg/ml), as compared to cisplatin and doxorubicin. Mellitin at 16 and 32μg/ml showed apoptotic effects on 4T1 cells according to the flow cytometric analysis. The Real time PCR analysis of Drp1 and Mfn1 expression in cells treated with 16μg/ml of melittin revealed an up-regulation in Drp1 and Mfn1 genes mRNA expression in comparison with control group. Treatment with 32μg/ml of melittin was also associated with a rise in mRNA expression of Drp1 and Mfn1 as compared to the control group. Conclusion: The results of this study showed that melittin has anticancer effects on 4T1 cell lines in a dose and time dependent manner and can be a good candidate for further research on breast cancer treatment.

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