Determination of the Developmental Origin of Seeds Containing Endosperm Using Flow Cytometric Analysis

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (11) ◽  
Author(s):  
Christian Sailer ◽  
Anja Schmidt ◽  
Ueli Grossniklaus
Keyword(s):  
Flow Cytometric Analysis ◽  
Developmental Origin ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet.

1997 ◽  
Vol 41 (12) ◽  
pp. 2686-2692 ◽  
Author(s):  
I Pavić ◽  
A Hartmann ◽  
A Zimmermann ◽  
D Michel ◽  
W Hampl ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cytopathic Effect ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Resistant Strains ◽  
Simplex Virus

We established a quantitative flow cytometric method for determination of herpes simplex virus type 1 (HSV-1) susceptibility to acyclovir (ACV), ganciclovir, and foscarnet in vitro. Susceptibility was defined in terms of the drug concentration which reduced the number of cells expressing HSV-1 glycoprotein C (gpC) with a fluorescence intensity of > or =10(2) by 50% (IC50). Flow cytometry allowed us to use a high (1.0) as well as a low (0.005) multiplicity of infection, and determination of the IC50 was possible after one or more viral replicative cycles. IC50s were dependent on virus input and on time postinfection. In mixture experiments, 1 to 2% resistant viruses added to a sensitive strain could be detected. The results obtained by flow cytometry showed a good qualitative correlation with those achieved by cytopathic effect inhibitory assay. However, flow cytometry might detect more quantitative differences in drug susceptibility, especially among resistant strains, as confirmed also by determination of intracellular drug phosphorylation. The mean IC50s for ACV-sensitive strains were 0.45 to 1.47 microM, and those for ACV-resistant strains were between 140 and 3,134 microM. Flow cytometric analysis was fast and accurate, automatizable, and highly reproducible. Flow cytometry may be a more powerful tool than standard cytopathic effect-based assays and could have advantages for the detection of low levels of drug resistance or mixtures of sensitive and resistant virus strains.

scholarly journals Determination of the Viability of Aeromonas hydrophila in Different Types of Water by Flow Cytometry, and Comparison with Classical Methods

2005 ◽  
Vol 71 (12) ◽  
pp. 7948-7954 ◽  
Author(s):  
Anna Pianetti ◽  
Tania Falcioni ◽  
Francesca Bruscolini ◽  
Luigia Sabatini ◽  
Elivio Sisti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Aeromonas Hydrophila ◽  
Flow Cytometric Analysis ◽  
Staining Technique ◽  
Plate Count ◽  
Physiological Status ◽  
Traditional Methods ◽  
Flow Cytometric ◽  
Bacterial Content

ABSTRACT The presence of Aeromonas spp. in water can represent a risk for human health. Therefore, it is important to know the physiological status of these bacteria and their survival in the environment. We studied the behavior of a strain of Aeromonas hydrophila in river water, spring water, brackish water, mineral water, and chlorinated drinking water, which had different physical and chemical characteristics. The bacterial content was evaluated by spectrophotometric and plate count techniques. Flow cytometric determination of viability was carried out using a dual-staining technique that enabled us to distinguish viable bacteria from damaged and membrane-compromised bacteria. The traditional methods showed that the bacterial content was variable and dependent on the type of water. The results obtained from the plate count analysis correlated with the absorbance data. In contrast, the flow cytometric analysis results did not correlate with the results obtained by traditional methods; in fact, this technique showed that there were viable cells even when the optical density was low or no longer detectable and there was no plate count value. According to our results, flow cytometry is a suitable method for assessing the viability of bacteria in water samples. Furthermore, it permits fast detection of bacteria that are in a viable but nonculturable state, which are not detectable by conventional methods.

Determination of residual T- and B-cell content after immunomagnetic depletion: proposal for flow cytometric analysis and results from 103 separations

Cytotherapy ◽  
2006 ◽  
Vol 8 (5) ◽  
pp. 465-472 ◽  
Author(s):  
M. Schumm ◽  
R. Handgretinger ◽  
M. Pfeiffer ◽  
T. Feuchtinger ◽  
S. Kuci ◽  
...  
Keyword(s):  
B Cell ◽  
Flow Cytometric Analysis ◽  
Cell Content ◽  
Flow Cytometric

scholarly journals Safe Determination of Susceptibility of Mycobacterium tuberculosis to Antimycobacterial Agents by Flow Cytometry

1999 ◽  
Vol 37 (3) ◽  
pp. 479-483 ◽  
Author(s):  
Andrea V. Moore ◽  
Scott M. Kirk ◽  
Steven M. Callister ◽  
Gerald H. Mazurek ◽  
Ronald F. Schell
Keyword(s):  
Flow Cytometry ◽  
Mycobacterium Tuberculosis ◽  
Flow Cytometric Analysis ◽  
Test Methods ◽  
Susceptibility Test ◽  
Flow Cytometric ◽  
Proportion Method ◽  
Bactec System ◽  
Drug Free

We showed previously that susceptibility testing forMycobacterium tuberculosis labeled with fluorescein diacetate could be accomplished rapidly by using flow cytometry. However, safety was a major concern because mycobacteria were not killed prior to flow cytometric analysis. In this study, we developed a biologically safe flow cytometric susceptibility test that depends on detection and enumeration of actively growing M. tuberculosis organisms in drug-free and antimycobacterial agent-containing medium. The susceptibilities of 17 clinical isolates of M. tuberculosis to ethambutol, isoniazid, and rifampin were tested by the agar proportion and flow cytometric methods. Subsequently, all flow cytometric susceptibility test samples were inactivated by exposure to paraformaldehyde before analysis with a flow cytometer. Agreement between the results from the two methods was 98%. In addition, the flow cytometric results were available 72 h after the initiation of testing. The flow cytometric susceptibility assay is safe, simple to perform, and more rapid than conventional test methods, such as the BACTEC system and the proportion method.

Determination of zygosity using flow cytometric analysis of red cell antigen strength

Transfusion ◽  
1988 ◽  
Vol 28 (6) ◽  
pp. 541-544 ◽  
Author(s):  
L Oien ◽  
S Nance ◽  
P Arndt ◽  
G Garratty
Keyword(s):  
Flow Cytometric Analysis ◽  
Red Cell ◽  
Cell Antigen ◽  
Flow Cytometric

Determination of Complement-Mediated Killing of Bacteria by Viability Staining and Bioluminescence

1998 ◽  
Vol 64 (2) ◽  
pp. 515-519 ◽  
Author(s):  
Marko Virta ◽  
Sanna Lineri ◽  
Pasi Kankaanpää ◽  
Matti Karp ◽  
Karita Peltonen ◽  
...  
Keyword(s):  
Cell Population ◽  
Flow Cytometric Analysis ◽  
Viable Cell ◽  
Genetically Engineered ◽  
Bacterial Viability ◽  
Viability Staining ◽  
Flow Cytometric ◽  
Genetically Engineered Bacteria ◽  
Conventional Plate

ABSTRACT Complement-mediated killing of bacteria was monitored by flow cytometric, luminometric, and conventional plate counting methods. A flow cytometric determination of bacterial viability was carried out by using dual staining with a LIVE/DEAD BacLight bacterial viability kit. In addition to the viable cell population, several other populations emerged in the fluorescence histogram, and there was a dramatic decrease in the total cell count in the light-scattering histogram in the course of the complement reaction. To permit luminometric measurements, Bacillus subtilis and Escherichia coli were made bioluminescent by expressing an insect luciferase gene. Addition of substrate after the complement reaction resulted in bioluminescence, the level of which was a measure of the viable cell population. All three methods gave essentially the same killing rate, suggesting that the bacteriolytic activity of serum complement can be measured rapidly and conveniently by using viability stains or bioluminescence. In principle, any bacterial strain can be used for viability staining and flow cytometric analysis. For the bioluminescence measurements genetically engineered bacteria are needed, but the advantage is that it is possible to screen automatically a large number of samples.

Quantitative determination of CD4/CD8 molecules by a cell marker ELISA

1994 ◽  
Vol 40 (1) ◽  
pp. 38-42 ◽  
Author(s):  
L Franke ◽  
E Nugel ◽  
W D Döcke ◽  
T Porstmann
Keyword(s):  
Flow Cytometry ◽  
T Lymphocytes ◽  
Flow Cytometric Analysis ◽  
Sample Volume ◽  
Small Sample ◽  
Cell Marker ◽  
Cell Counts ◽  
Flow Cytometric ◽  
A Cell

Abstract Determination of percentages of CD4+ and CD8+ T cells from patients with human immunodeficiency virus infection is usually done by flow cytometric analysis. We compared a cell marker ELISA with flow cytometry for quantitation of CD4 and CD8 molecules on T lymphocytes, and correlated the values both with the number of CD4+ and CD8+ T lymphocytes and with clinical data. Results by cell marker ELISA (y) correlated well with those by flow cytometric analysis (x); r = 0.69, P < 0.001 (y = 0.01x + 3.9) for CD4; r = 0.81, P < 0.001 (y = 0.03x + 5.4) for CD8; n = 343. The ELISA detected changes in numbers of CD8 molecules on the cells earlier than flow cytometry recognized changes in CD8+ T-cell counts. The advantages of the ELISA are the small sample volume required (0.5 mL of blood), its internal standardization by a CD4+/CD8+ cell line, and its simple and fast performance. The cell marker ELISA appears to be an efficient alternative to flow cytometry.

Sign in / Sign up

Export Citation Format

Share Document