Effects of hyaluronic acid and tacrolimus on the prevention of perineural scar formation and on nerve regeneration after sciatic nerve repair in a rabbit model

2016 ◽  
Vol 43 (4) ◽  
pp. 497-504 ◽  
Author(s):  
A. Y. Mekaj ◽  
S. Manxhuka-Kerliu ◽  
A. A. Morina ◽  
S. B. Duci ◽  
L. Shahini ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Repair ◽  
Rabbit Model ◽  
Scar Formation

scholarly journals Effect of Hyaluronic Acid-Carboxymethylcellulose Solution on Perineural Scar Formation after Sciatic Nerve Repair in Rats

2011 ◽  
Vol 3 (4) ◽  
pp. 315 ◽  
Author(s):  
Jin Sung Park ◽  
Jae Hoon Lee ◽  
Chung Soo Han ◽  
Duke Whan Chung ◽  
Gou Young Kim
Keyword(s):  
Hyaluronic Acid ◽  
Sciatic Nerve ◽  
Nerve Repair ◽  
Scar Formation

scholarly journals IL-17F depletion accelerates chitosan conduit guided peripheral nerve regeneration

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Feixiang Chen ◽  
Weihuang Liu ◽  
Qiang Zhang ◽  
Ping Wu ◽  
Ao Xiao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Knockout Mice ◽  
Inflammatory Markers ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Wild Type ◽  
Anti Inflammatory

AbstractPeripheral nerve injury is a serious health problem and repairing long nerve deficits remains a clinical challenge nowadays. Nerve guidance conduit (NGC) serves as the most promising alternative therapy strategy to autografts but its repairing efficiency needs improvement. In this study, we investigated whether modulating the immune microenvironment by Interleukin-17F (IL-17F) could promote NGC mediated peripheral nerve repair. Chitosan conduits were used to bridge sciatic nerve defect in IL-17F knockout mice and wild-type mice with autografts as controls. Our data revealed that IL-17F knockout mice had improved functional recovery and axonal regeneration of sciatic nerve bridged by chitosan conduits comparing to the wild-type mice. Notably, IL-17F knockout mice had enhanced anti-inflammatory macrophages in the NGC repairing microenvironment. In vitro data revealed that IL-17F knockout peritoneal and bone marrow derived macrophages had increased anti-inflammatory markers after treatment with the extracts from chitosan conduits, while higher pro-inflammatory markers were detected in the Raw264.7 macrophage cell line, wild-type peritoneal and bone marrow derived macrophages after the same treatment. The biased anti-inflammatory phenotype of macrophages by IL-17F knockout probably contributed to the improved chitosan conduit guided sciatic nerve regeneration. Additionally, IL-17F could enhance pro-inflammatory factors production in Raw264.7 cells and wild-type peritoneal macrophages. Altogether, IL-17F may partially mediate chitosan conduit induced pro-inflammatory polarization of macrophages during nerve repair. These results not only revealed a role of IL-17F in macrophage function, but also provided a unique and promising target, IL-17F, to modulate the microenvironment and enhance the peripheral nerve regeneration.

scholarly journals Learning curve in rat dissection for experimental sciatic nerve repair

2019 ◽  
pp. 243-248
Author(s):  
Marin Andrei ◽  
Marin Georgiana Gabriela ◽  
Dobrete Nicoleta Amalia ◽  
Enescu Dan Mircea
Keyword(s):  
Sciatic Nerve ◽  
Learning Curve ◽  
Nerve Regeneration ◽  
Experimental Model ◽  
Rat Model ◽  
Surgical Intervention ◽  
Nerve Repair ◽  
Learning Curves ◽  
Rat Sciatic Nerve ◽  
Nerve Surgery

The baseline for any key research in nerve regeneration is an experimental model and the sciatic nerve in the rat model is the workhorse in this field. Although physically resistant to external traumas, a surgical intervention constitutes a major distress even for a rat. In the following presentation, we will analyse the learning curves for different stages in the rat sciatic nerve surgery as well as possible factors which influence these times.

scholarly journals Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

2018 ◽  
Vol 6 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
C. R. Carvalho ◽  
S. Wrobel ◽  
C. Meyer ◽  
C. Brandenberger ◽  
I. F. Cengiz ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Work ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Gellan Gum ◽  
In Vivo Study ◽  
Rat Sciatic Nerve

This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels.

scholarly journals Mesenchymal Stem Cells Enhance Nerve Regeneration in a Rat Sciatic Nerve Repair and Hindlimb Transplant Model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Damon S. Cooney ◽  
Eric G. Wimmers ◽  
Zuhaib Ibrahim ◽  
Johanna Grahammer ◽  
Joani M. Christensen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Repair ◽  
Rat Sciatic Nerve ◽  
Transplant Model

scholarly journals Electrospun polycaprolactone (PCL)-amnion nanofibrous membrane prevents adhesions and promotes nerve repair in a rat model of sciatic nerve compression

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244301
Author(s):  
Ruiyi Dong ◽  
Chunjie Liu ◽  
Siyu Tian ◽  
Jiangbo Bai ◽  
Kunlun Yu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Rat Model ◽  
Nerve Repair ◽  
Adhesion Formation ◽  
Nerve Compression ◽  
Nanofibrous Membrane ◽  
Control Group ◽  
Chitosan Hydrogel

Adhesion and scarring after neural surgery are detrimental to nerve regeneration and functional recovery. Amniotic membranes have been used in tissue repair due to their immunogenicity and richness in cytokines. In this study, an electrospun polycaprolactone (PCL)-amnion nanofibrous membrane was prepared for the treatment of sciatic nerve compression in a rat model. The effects of the PCL-amnion nanofibrous membrane on the prevention of adhesion formation and nerve regeneration were evaluated using electrophysiology and histological analyses. Compared with the medical chitosan hydrogel dressing, the PCL-amnion nanofibrous membrane significantly reduced peripheral nerve adhesion and promoted the rapid recovery of nerve conduction. Moreover, the immunohistochemical analysis identified more Schwann cells and less pro-inflammatory M1 macrophages in the PCL-amnion group. Western blot and RT-PCR results showed that the expression levels of type-Ⅰ and Ⅲ collagen in the PCL-treated rats were half of those in the control group after 12 weeks, while the expression level of nerve growth factor was approximately 3.5 times that found in the rats treated with medical chitosan hydrogel. In summary, electrospun PCL-amnion nanofibrous membranes can effectively reduce adhesion after neural surgery and promote nerve repair and regeneration. The long-term retention in vivo and sustained release of cytokines make PCL-amnion a promising biomaterial for clinical application.

Comparative Study of Scar Formation at the Site of Sciatic Nerve Repair in Rats

2007 ◽  
Vol 42 (2) ◽  
pp. 162 ◽  
Author(s):  
Kwang Suk Lee ◽  
Jong Ryoon Baek ◽  
Gyou Hyuk Lee ◽  
Gi Won Choi
Keyword(s):  
Sciatic Nerve ◽  
Comparative Study ◽  
Nerve Repair ◽  
Scar Formation

Stimulated Jitter Measurement in the Assessment of Recovery after different Methods of Peripheral Nerve Repair

1998 ◽  
Vol 23 (1) ◽  
pp. 12-16 ◽  
Author(s):  
D. V. LENIHAN ◽  
N. M. SOJITRA ◽  
M. A. GLASBY
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Neuromuscular Junctions ◽  
Nerve Repair ◽  
Neuromuscular Function ◽  
Electrophysiological Recording ◽  
Peripheral Nerve Repair ◽  
Future Developments ◽  
Jitter Measurement

The recording of stimulated jitter offers a quantitative method for following the recovery of neuromuscular function after peripheral nerve repair. In groups of rats, electrophysiological recording of jitter was carried out on control animals and on animals 90 days after sciatic nerve division and subsequent repair with either direct end-to-end suture (NS), nerve graft (NG) or freeze thawed muscle graft (FTMG). It was found that values for jitter were highest in the FTMG group. The NS and NG groups demonstrated statistically similar jitter values when compared with each other and with the normal. It was concluded that the speed of nerve regeneration is slower in the FTMG group, at least initially, and that 90 days after sciatic nerve repair the FMTG group had an increase in the number of immature neuromuscular junctions when compared with the NS or NG groups. Jitter measurement would appear to offer a means of detecting small differences in nerve regeneration. The value of this in future developments in nerve repair is discussed.

Sign in / Sign up

Export Citation Format

Share Document