Designer lymphocytes to fight cancer: a helping hand from modern molecular biology

In 1990, Charles Mackay and colleagues combined classical physiology with modern molecular biology to provide the first concrete evidence that naive and memory T cells follow distinct migratory routes out of the bloodstream— a discovery that helped invigorate the field of lymphocyte homing.

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Anniversary of the Theory of Academician E.N. Pavlovsky about the Natural Focality of Diseases (1939 - 2014)

Epidemiology and Vaccinal Prevention ◽

10.31631/2073-3046-2015-14-1-9-16 ◽

2015 ◽

Vol 14 (1) ◽

pp. 9-16 ◽

Cited By ~ 1

Author(s):

E. I. Korenberg

Keyword(s):

Molecular Biology ◽

75Th Anniversary ◽

Modern Molecular Biology ◽

Natural Foci ◽

Ecological Methods ◽

Traditional Population

In connection with the 75th anniversary of the theory of academician E.N. Pavlovsky about the natural focality of diseases presents the General results of the development of its fundamental theoretical positions. Planned most promising areas for further research, which can immeasurably increase when the combination of modern molecular biology and traditional population-ecological methods for the study of natural foci.

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RNA-Targeting CRISPR–Cas Systems and Their Applications

International Journal of Molecular Sciences ◽

10.3390/ijms21031122 ◽

2020 ◽

Vol 21 (3) ◽

pp. 1122 ◽

Cited By ~ 7

Author(s):

Michal Burmistrz ◽

Kamil Krakowski ◽

Agata Krawczyk-Balska

Keyword(s):

Molecular Biology ◽

Current Knowledge ◽

Type Ii ◽

Rna Molecules ◽

Dna Targeting ◽

Modern Molecular Biology ◽

Rna Targeting ◽

New Research ◽

Target Rna ◽

Research Tools

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR–Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems.

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Who Pooped and How It Was Found

Water in Kentucky ◽

10.5810/kentucky/9780813168685.003.0018 ◽

2017 ◽

Author(s):

Tricia Coakley

Keyword(s):

Molecular Biology ◽

Real World ◽

Cost Effective ◽

Fecal Pollution ◽

Important Task ◽

Source Tracking ◽

Fecal Bacteria ◽

Non Profit ◽

Pollution Problem ◽

Modern Molecular Biology

Tracking the sources of fecal pollution in Kentucky streams is an important task to allow for removal of the associated pollution. Modern molecular biology techniques, analyzing the DNA of fecal bacteria, provide cost effective methods and make fecal source tracking possible for municipalities and non-profit organizations. This is a story of the use of these methods by a collaboration of stakeholders to solve a real world fecal pollution problem in Lexington, Kentucky.

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Parasites and their (endo)symbiotic microbes

Parasitology ◽

10.1017/s0031182018001257 ◽

2018 ◽

Vol 145 (10) ◽

pp. 1261-1264 ◽

Cited By ~ 4

Author(s):

Vyacheslav Yurchenko ◽

Julius Lukeš

Keyword(s):

Molecular Biology ◽

Microbial Ecology ◽

Host Cell ◽

High Frequency ◽

Symbiotic Bacteria ◽

Biological Complexity ◽

Comparative Analyses ◽

Modern Molecular Biology ◽

The Impact

AbstractThanks to modern molecular biology methods, our understanding of the impact of (endo)symbiotic bacteria on parasitic protists and helminths is growing fast. In this issue, 9 papers have been brought together that describe various facets of the relationships between these microorganisms, reveal their range and high frequency, as well as their capacity to create novel biological complexity. Comparative analyses of these host–endosymbiont interactions indicate that there may be no discrete types of relationships but rather a continuum ranging from a dispensable endosymbiont minimally integrated within the host cell to organelles, such as mitochondria and plastids that evolved into an indispensable, deeply integrated components of the cell. We hope that this series of studies on parasites and (endo)symbiotic bacteria will increase awareness about these relationships and their representation in microbial ecology models.

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Bringing the physical sciences into your cell biology research

Molecular Biology of the Cell ◽

10.1091/mbc.e12-05-0354 ◽

2012 ◽

Vol 23 (21) ◽

pp. 4167-4170

Author(s):

Douglas N. Robinson ◽

Pablo A. Iglesias

Keyword(s):

Molecular Biology ◽

Cell Biology ◽

Interdisciplinary Approach ◽

Scientific Discipline ◽

Living Systems ◽

Molecular Pathways ◽

Physical Sciences ◽

Molecular Approaches ◽

Modern Molecular Biology ◽

Biology Research

Historically, much of biology was studied by physicists and mathematicians. With the advent of modern molecular biology, a wave of researchers became trained in a new scientific discipline filled with the language of genes, mutants, and the central dogma. These new molecular approaches have provided volumes of information on biomolecules and molecular pathways from the cellular to the organismal level. The challenge now is to determine how this seemingly endless list of components works together to promote the healthy function of complex living systems. This effort requires an interdisciplinary approach by investigators from both the biological and the physical sciences.

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