Comparative genome-wide mapping versus extreme pool-genotyping and development of diagnostic SNP markers linked to QTL for adult plant resistance to stripe rust in common wheat

2018 ◽  
Vol 131 (8) ◽  
pp. 1777-1792 ◽  
Author(s):  
Jianhui Wu ◽  
Shuo Huang ◽  
Qingdong Zeng ◽  
Shengjie Liu ◽  
Qilin Wang ◽  
...  
Keyword(s):  
Common Wheat ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Snp Markers ◽  
Adult Plant ◽  
Comparative Genome ◽  
Genome Wide ◽  
Pool Genotyping ◽  
Mapping Versus

Molecular Mapping of a Novel QTL Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace ‘Guangtoumai’

Plant Disease ◽  
2020 ◽  
Author(s):  
Yu Wu ◽  
Yuqi Wang ◽  
Fangjie Yao ◽  
Li Long ◽  
Jing Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Major Locus ◽  
Dcaps Marker ◽  
Chinese Wheat

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Chinese wheat landrace ‘Guangtoumai’ (GTM) exhibited a high-level resistance against predominant Pst races in China at the adult-plant stage. The objective of this research was to identify and map the major locus/loci for stripe rust resistance in GTM. A set of 212 recombinant inbred lines (RILs) was developed from a cross between GTM and Avocet S (AvS). The parents and RILs were evaluated in three field tests (2018, 2019, and 2020 at Chongzhou, Sichuan) with the currently predominant Pst races for final disease severity (FDS) and genotyped with the Wheat 55K SNP array to construct a genetic map with 1,031 SNP markers. A major locus, named QYr.GTM-5DL, was detected on chromosome 5DL in GTM. The locus was mapped in a 2.75 cM interval flanked by SNP markers AX-109855976 and AX-109453419, explaining up to 44.4% of the total phenotypic variation. Since no known Yr genes have been reported on chromosome 5DL, QYr.GTM-5DL is very likely a novel adult plant resistance (APR) locus. Haplotype analysis revealed that the resistance allele displayed enhanced levels of stripe rust resistance and is likely present in 5.3% of the 247 surveyed Chinese wheat landraces. The derived cleaved amplified polymorphic sequence (dCAPS) marker dCAPS-5722, converted from a SNP marker tightly linked to QYr.GTM-5DL with 0.3 cM, was validated on a subset of RILs and 48 commercial wheat cultivars developed in Sichuan. The results indicated that QYr.GTM-5DL with its linked dCAPS marker could be used in marker-assisted selection to improve stripe rust resistance in breeding programs, and this QTL will provide new and possibly durable resistance to stripe rust.

scholarly journals QTL Mapping for Adult-Plant Resistance to Stripe Rust in a Common Wheat RIL Population Derived from Chuanmai 32/Chuanyu 12

2012 ◽  
Vol 11 (11) ◽  
pp. 1775-1782 ◽  
Author(s):  
Ling WU ◽  
Xian-chun XIA ◽  
You-liang ZHENG ◽  
Zheng-yu ZHANG ◽  
Hua-zhong ZHU ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Common Wheat ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Ril Population

scholarly journals Genome-Wide Linkage Mapping of QTL for Adult-Plant Resistance to Stripe Rust in a Chinese Wheat Population Linmai 2 × Zhong 892

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145462 ◽  
Author(s):  
Jindong Liu ◽  
Zhonghu He ◽  
Ling Wu ◽  
Bin Bai ◽  
Weie Wen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Linkage Mapping ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Wheat Population ◽  
Genome Wide ◽  
Chinese Wheat

QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli

2009 ◽  
Vol 119 (8) ◽  
pp. 1349-1359 ◽  
Author(s):  
Yaming Lu ◽  
Caixia Lan ◽  
Shanshan Liang ◽  
Xiangchun Zhou ◽  
Di Liu ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Common Wheat ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

scholarly journals Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line

2021 ◽  
Author(s):  
Minghu Zhang ◽  
Xin Liu ◽  
Ting Peng ◽  
Dinghao Wang ◽  
Dongyu Liang ◽  
...  
Keyword(s):  
Common Wheat ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Recessive Gene ◽  
Introgression Line ◽  
Physical Region ◽  
Adult Plant ◽  
Triticum Boeoticum

Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy. Triticum boeoticum Boiss (T. monococcum L. ssp. aegilopoides, 2n = 2x = 14, AbAb) accession G52 confers a high level of adult-plant resistance against a mixture of the Chinese prevalent Pst races. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat-T. boeoticum introgression line Z15-1370 (F5 generation) with 42 chromosomes was selected cytologically and by testing with Pst races. In order to map the resistance gene(s), F1, F2, and F2:3 generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, temporarily designated YrZ15-1370. Using the bulked segregant RNA-Seq (BSR-Seq) analysis, YrZ15-1370 was mapped to chromosome 6AL and flanked by markers KASP1370-3 and KASP-1370-5 within a 4.3 cM genetic interval corresponding to 1.8 Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated. YrZ15-1370 differed from previously Yr genes identified on chromosome 6A based on its position and/or origin. The YrZ15-1370 would be a valuable resource for wheat resistance improvement and the flanking markers developed here should be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene.

scholarly journals Identification and Mapping of Adult Plant Resistance Loci to Leaf Rust and Stripe Rust in Common Wheat Cultivar Kundan

Plant Disease ◽  
2017 ◽  
Vol 101 (3) ◽  
pp. 456-463 ◽  
Author(s):  
Y. Ren ◽  
R. P. Singh ◽  
B. R. Basnet ◽  
C. X. Lan ◽  
J. Huerta-Espino ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Adult Plant Resistance ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Snp Markers ◽  
Adult Plant ◽  
Additive Interaction

Leaf rust (LR) and stripe rust (YR) are important diseases of wheat worldwide. We used 148 recombinant inbred lines (RIL) from the cross of Avocet × Kundan for determining and mapping the genetic basis of adult plant resistance (APR) loci. The population was phenotyped LR and YR for three seasons in field trials conducted in Mexico and genotyped with the diversity arrays technology sequencing (DArT-Seq) and simple sequence repeat markers. The final genetic map was constructed using 2,937 polymorphic markers with an average distance of 1.29 centimorgans between markers. Inclusive composite interval mapping identified two co-located APR quantitative trait loci (QTL) for LR and YR, two LR QTL, and three YR QTL. The co-located resistance QTL on chromosome 1BL corresponded to the pleiotropic APR gene Lr46/Yr29. QLr.cim-2BL, QYr.cim-2AL, and QYr.cim-5AS could be identified as new resistance loci in this population. Lr46/Yr29 contributed 49.5 to 65.1 and 49.2 to 66.1% of LR and YR variations, respectively. The additive interaction between detected QTL showed that LR severities for RIL combining four QTL ranged between 5.3 and 25.8%, whereas the lowest YR severities were for RIL carrying QTL on chromosomes 1BL + 2AL + 6AL. The high-density DArT-Seq markers across chromosomes can be used in fine mapping of the targeted loci and development SNP markers.

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