A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat

SummaryBread wheat (Triticum aestivum L.) exhibits very narrow genetic diversity and hence there is high relatedness among cultivated varieties. However, a population generated from an intervarietal cross, with the parents differing in a large number of traits, could lead to the generation of QTL maps which will be useful in practice. In this report a genetic linkage map of wheat is constructed using a cross between two Indian bread wheat varieties: Sonalika and Kalyansona. The linkage map consisted of 236 markers and spanned a distance of 3639 cM, with 1211·2 cM for the A genome, 1669·2 cM for the B genome, 192·4 cM for the D genome and 566·2 cM for unassigned groups. Linkage analysis defined 37 linkage groups of which 24 were assigned to 17 chromosomes. The genetic map was used to identify QTLs by composite internal mapping (CIM) for three metric traits, viz. culm length (CL), flag leaf length (FLL) and flag leaf breadth (FLB). Of 25 QTLs identified in this study, 15 have not been reported previously. Multitrait CIM (MCIM) analysis was carried out for traits that were significantly correlated such as FLB–FLL and CL–FLB–FLL. Detection of a large number of QTLs for the three traits analysed suggests that in parent cultivars that are not too diverse, the differences at genetic level detected as polymorphisms may be mostly associated with QTLs for the observed differences.

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Construction of a high-density genetic linkage map and identification of gene controlling resistance to cucumber mosaic virus in Luffa cylindrica (L.) Roem. based on specific length amplified fragment sequencing

Molecular Biology Reports ◽

10.1007/s11033-020-05652-8 ◽

2020 ◽

Vol 47 (8) ◽

pp. 5831-5841

Author(s):

Lina Lou ◽

Xiaojun Su ◽

Xiaohong Liu ◽

Zhe Liu

Keyword(s):

Linkage Map ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

High Density ◽

Luffa Cylindrica ◽

Specific Length

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Construction of a high‐density genetic linkage map and identification of QTLs for main agronomic traits of tetraploid hybrid crested wheatgrass

Grassland Science ◽

10.1111/grs.12265 ◽

2020 ◽

Vol 66 (3) ◽

pp. 161-173 ◽

Cited By ~ 1

Author(s):

Xiaoxia Yu ◽

Yanhong Ma ◽

Zhiyan Jiang ◽

Yue Shi ◽

Dongsheng Yang ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Agronomic Traits ◽

High Density ◽

Crested Wheatgrass ◽

Tetraploid Hybrid

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A high-density SNP-based genetic map and several economic traits-related loci in Pelteobagrus vachelli

BMC Genomics ◽

10.1186/s12864-020-07115-7 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Guosong Zhang ◽

Jie Li ◽

Jiajia Zhang ◽

Xia Liang ◽

Tao Wang ◽

...

Keyword(s):

Sex Determination ◽

Linkage Map ◽

Candidate Genes ◽

Genetic Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

High Density ◽

Hypoxia Tolerance ◽

Genome Wide ◽

Pelteobagrus Vachelli

Abstract Background A high-density genetic linkage map is essential for QTL fine mapping, comparative genome analysis, identification of candidate genes and marker-assisted selection in aquaculture species. Pelteobagrus vachelli is a very popular commercial species in Asia. However, some specific characters hindered achievement of the traditional selective breeding based on phenotypes, such as lack of large-scale genomic resource and short of markers tightly associated with growth, sex determination and hypoxia tolerance related traits. Results By making use of 5059 ddRAD markers in P. vachelli, a high-resolution genetic linkage map was successfully constructed. The map’ length was 4047.01 cM by using an interval of 0.11 cm, which is an average marker standard. Comparative genome mapping revealed that a high proportion (83.2%) of markers with a one-to-one correspondence were observed between P. vachelli and P. fulvidraco. Based on the genetic map, 8 significant genome-wide QTLs for 4 weight, 1 body proportion, 2 sex determination, and 1 hypoxia tolerance related traits were detected on 4 LGs. Some SNPs from these significant genome-wide QTLs were observably associated with these phenotypic traits in other individuals by Kompetitive Allele Specific PCR. In addition, two candidate genes for weight, Sipa1 and HSD11B2, were differentially expressed between fast-, medium- and slow-growing P. vachelli. Sema7a, associated with hypoxia tolerance, was induced after hypoxia exposure and reoxygenation. Conclusions We mapped a set of suggestive and significant QTLs as well as candidate genes for 12 growth, 1 sex determination and 1 hypoxia tolerance related traits based on a high-density genetic linkage map by making use of SNP markers for P. fulvidraco. Our results have offered a valuable method about the much more efficient production of all-male, fast growth and hypoxia tolerance P. vachelli for the aquaculture industry.

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A High-Density Genetic Linkage Map and QTL Mapping for Sex and Growth-Related Traits of Large-Scale Loach (Paramisgurnus dabryanus)

Frontiers in Genetics ◽

10.3389/fgene.2019.01023 ◽

2019 ◽

Vol 10 ◽

Author(s):

Jin Wei ◽

Yuanyuan Chen ◽

Weimin Wang

Keyword(s):

Qtl Mapping ◽

Linkage Map ◽

Genetic Linkage ◽

Large Scale ◽

Genetic Linkage Map ◽

High Density ◽

Paramisgurnus Dabryanus

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Construction of a high-density genetic linkage map and QTL analysis for hazelnut breeding

Acta Horticulturae ◽

10.17660/actahortic.2018.1226.3 ◽

2018 ◽

pp. 25-30

Author(s):

D. Torello Marinoni ◽

N. Valentini ◽

E. Portis ◽

A. Acquadro ◽

C. Beltramo ◽

...

Keyword(s):

Linkage Map ◽

Qtl Analysis ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

High Density

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Construction of a high-density linkage map and QTL analysis for pistil abortion in apricot (Prunus armeniaca L.)

Canadian Journal of Plant Science ◽

10.1139/cjps-2018-0177 ◽

2019 ◽

Vol 99 (5) ◽

pp. 599-610

Author(s):

Junhuan Zhang ◽

Haoyuan Sun ◽

Li Yang ◽

Fengchao Jiang ◽

Meiling Zhang ◽

...

Keyword(s):

Linkage Map ◽

Genetic Map ◽

Qtl Analysis ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Prunus Armeniaca ◽

High Density ◽

Pistil Abortion ◽

Genomic Study ◽

Prunus Armeniaca L

A high-density genetic map of apricot (Prunus armeniaca L.) was constructed using an F1 population constructed by crossing two main Chinese cultivars ‘Chuanzhihong’ and ‘Luotuohuang’, coupled with a recently developed reduced representation library (RRL) sequencing. The average sequencing depth was 38.97 in ‘Chuanzhihong’ (female parent), 33.05 in ‘Luotuohuang’ (male parent), and 8.91 in each progeny. Based on the sequencing data, 12 451 polymorphic markers were developed and used in the construction of the genetic linkage map. The final map of apricot comprised eight linkage groups, including 1991 markers, and covered 886.25 cM of the total map length. The average distance between adjacent markers was narrowed to 0.46 cM. Gaps larger than 5 cM only accounted for <0.33%. To our knowledge, this map is the densest genetic linkage map that is currently available for apricot research. It is a valuable linkage map for quantitative trait loci (QTLs) identification of important agronomic traits. Moreover, the high marker density and well-ordered markers that this linkage map provides will be useful for molecular breeding of apricot as well. In this study, we applied this map in the QTL analysis of an important agronomic trait, pistil abortion. Several QTLs were detected and mapped respectively to the middle regions of LG5 (51.005∼59.4 cM) and LG6 (72.884∼76.562 cM), with nine SLAF markers closely linked to pistil abortion. The high-density genetic map and QTLs detected in this study will facilitate marker-assisted breeding and apricot genomic study.

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