Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in a doubled-haploid population of barley

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1221-1232 ◽  
Author(s):  
H. Zhu ◽  
L. Gilchrist ◽  
P. Hayes ◽  
A. Kleinhofs ◽  
D. Kudrna ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Plant Height ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Head Blight ◽  
Haploid Population ◽  
Fhb Resistance

The influence of Fusarium head blight on phenotypic distribution of barley doubled haploid population in respect of yield-related traits

2000 ◽  
Vol 28 (4) ◽  
pp. 485-492
Author(s):  
M. Surma ◽  
Z. Kaczmarek ◽  
T. Adamski ◽  
J. Chełkowski ◽  
H. Wiśniewska
Keyword(s):  
Fusarium Head Blight ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Head Blight ◽  
Phenotypic Distribution ◽  
Haploid Population

Molecular Mapping of QTL for Fusarium Head Blight Resistance in a Doubled Haploid Population of Chinese Bread Wheat

Plant Disease ◽  
2020 ◽  
Author(s):  
Zhanwang Zhu ◽  
Xiaoting Xu ◽  
Luping Fu ◽  
Fengju Wang ◽  
Yachao Dong ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Plant Height ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Natural Infection ◽  
Snp Array ◽  
Head Blight ◽  
Dh Population ◽  
Anther Extrusion ◽  
Fhb Resistance

Fusarium head blight (FHB) is a destructive disease of wheat worldwide, particularly in China. To map genetic loci underlying FHB resistance, a doubled haploid (DH) population consisting of 174 lines was developed from a cross between widely grown Chinese cultivars Yangmai 16 and Zhongmai 895. The DH population and parents were evaluated in field nurseries at Wuhan in 2016–2017 and 2017–2018 with both spray inoculation and natural infection, and at Jingzhou in 2017–2018 with grain-spawn inoculation. The DH lines were genotyped with a wheat 660K SNP array. FHB index, plant height, anther extrusion, and days to anthesis were recorded and used for QTL analysis. Seven QTL for FHB resistance were mapped to chromosome arms 3BL, 4AS, 4BS, 4DS, 5AL, 6AL, and 6BS, in at least two environments. QFhb.caas-4BS and QFhb.caas-4DS co-located with semi-dwarfing alleles Rht-B1b and Rht-D1b, respectively, and were also associated with anther extrusion. The other five QTL were genetically independent of the agronomic traits, indicating their potential value in breeding for FHB resistance. Based on correlations between FHB indices and agronomic traits in this population, we concluded that increasing plant height to some extent would enhance FHB resistance, anther extrusion played a more important role in environments with less severe FHB, and days to anthesis was independent of FHB response when viewed across years. PCR-based markers were developed for the 3BL and 5AL QTL, which were detected in more than three environments. The InDel marker InDel_AX-89588684 for QFhb.caas-5AL was also validated on a wheat panel, confirming its effectiveness for marker-assisted breeding for improvement of FHB resistance.

scholarly journals Characterization of QTL and eQTL controlling early Fusarium graminearum infection and deoxynivalenol levels in a Wuhan 1 x Nyubai doubled haploid wheat population

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
François Fauteux ◽  
Yunli Wang ◽  
Hélène Rocheleau ◽  
Ziying Liu ◽  
Youlian Pan ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Doubled Haploid ◽  
Expression Patterns ◽  
Doubled Haploid Population ◽  
Post Inoculation ◽  
Head Blight ◽  
Wheat Genotypes ◽  
Wheat Population ◽  
Fhb Resistance ◽  
Moderately Resistant

Abstract Background Fusarium head blight (FHB) is a major disease of cereal crops, caused by the fungal pathogen Fusarium graminearum and related species. Breeding wheat for FHB resistance contributes to increase yields and grain quality and to reduce the use of fungicides. The identification of genes and markers for FHB resistance in different wheat genotypes has nevertheless proven challenging. Results In this study, early infection by F. graminearum was analyzed in a doubled haploid population derived from the cross of the moderately resistant wheat genotypes Wuhan 1 and Nyubai. Three quantitative trait loci (QTL) were identified: 1AL was associated with lower deoxynivalenol content, and 4BS and 5A were associated with reduced F. graminearum infection at 2 days post inoculation. Early resistance alleles were inherited from Wuhan 1 for QTL 1AL and 4BS and inherited from Nyubai for the 5A QTL. Cis and trans expression QTL (eQTL) were identified using RNA-seq data from infected head samples. Hotspots for trans eQTL were identified in the vicinity of the 1AL and 4BS QTL peaks. Among differentially expressed genes with cis eQTL within the QTL support intervals, nine genes had higher expression associated with FHB early resistance, and four genes had higher expression associated with FHB early susceptibility. Conclusions Our analysis of genotype and gene expression data of wheat infected by F. graminearum identified three QTL associated with FHB early resistance, and linked genes with eQTL and differential expression patterns to those QTL. These findings may have applications in breeding wheat for early resistance to FHB.

scholarly journals The Semidwarfing Alleles Rht-D1b and Rht-B1b Show Marked Differences in Their Associations with Anther-Retention in Wheat Heads and with Fusarium Head Blight Susceptibility

2016 ◽  
Vol 106 (12) ◽  
pp. 1544-1552 ◽  
Author(s):  
Maria Buerstmayr ◽  
Hermann Buerstmayr
Keyword(s):  
Fusarium Head Blight ◽  
Plant Height ◽  
Fusarium Graminearum ◽  
Hyphal Growth ◽  
Field Trials ◽  
Wheat Breeding ◽  
Head Blight ◽  
Backcross Population ◽  
Reduced Height ◽  
Haploid Population

The semidwarfing alleles reduced height (Rht)-D1b and Rht-B1b are widely deployed in wheat breeding. Both alleles have similar effects on plant height but differ in their effect on Fusarium head blight (FHB) severity. A double-haploid population and a backcross population, segregating for Rht-B1a/Rht-B1b and Rht-D1a/Rht-D1b, were evaluated for FHB severity, plant height, and anther retention in field trials in three consecutive years. The semidwarfing alleles reduced plant height and increased the proportion of retained anthers. Reduced plant height and a high proportion of retained anthers were associated with increased FHB severity. The Rht-D1b allele had a significantly greater impact on anther retention and FHB severity than the Rht-B1b allele. Fusarium graminearum establishes infection sites predominantly inside the floral cavity and retained anthers potentially support colonization and initial hyphal growth, leading to a higher disease level in genotypes with a higher proportion of retained anthers. This is the first report demonstrating that differences in disease severity associated with Rht-D1b and Rht-B1b can be partly explained by their different effect on the extent of anther retention.

Mapping QTLs with epistatic effects and QTL × environment interactions for plant height using a doubled haploid population in cultivated wheat

2008 ◽  
Vol 35 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Kunpu Zhang ◽  
Jichun Tian ◽  
Liang Zhao ◽  
Shanshan Wang
Keyword(s):  
Plant Height ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Epistatic Effects ◽  
Haploid Population

Genetic variation for resistance to Fusarium head blight in winter durum material

2014 ◽  
Vol 65 (1) ◽  
pp. 46 ◽  
Author(s):  
Thomas Miedaner ◽  
Carl Friedrich Horst Longin
Keyword(s):  
Genetic Variation ◽  
Fusarium Head Blight ◽  
Plant Height ◽  
Fusarium Culmorum ◽  
Heading Time ◽  
Head Blight ◽  
Breeding Lines ◽  
Breeding Material ◽  
Quantitative Genetic ◽  
Fhb Resistance

Durum is one of the most susceptible cereals to infection with Fusarium head blight (FHB) due to the lack of resistance sources. Data on the genetic variation and heritability of FHB in elite durum, and especially the new evolving winter durum, are lacking. Thus, we compared 105 elite, winter durum breeding lines with an international collection of 62 old and new winter durum cultivars. We evaluated the development of FHB after inoculation by Fusarium culmorum, heading time, and plant height at three field environments in Germany. Significant genetic variation for FHB was identified in the elite breeding material as well as in the collection. Mean FHB rating was normally distributed with a heritability of >0.7 for both sets, indicating the quantitative genetic nature of the trait. Taking heading time, plant height, and FHB resistance into account, the most interesting genotypes were identified in the elite breeding material. Consequences for the ongoing global efforts for improvement of FHB resistance of durum are discussed.

Molecular markers linked to genes affecting plant height in wheat using a doubled-haploid population

1998 ◽  
Vol 96 (6-7) ◽  
pp. 933-940 ◽  
Author(s):  
T. Cadalen ◽  
P. Sourdille ◽  
G. Charmet ◽  
M. H. Tixier ◽  
G. Gay ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Plant Height ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Haploid Population

scholarly journals Characterization of the Genetic Architecture for Fusarium Head Blight Resistance in Durum Wheat: The Complex Association of Resistance, Flowering Time, and Height Genes

2020 ◽  
Vol 11 ◽  
Author(s):  
Yuefeng Ruan ◽  
Wentao Zhang ◽  
Ron E. Knox ◽  
Samia Berraies ◽  
Heather L. Campbell ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Fusarium Head Blight ◽  
Flowering Time ◽  
Plant Height ◽  
Genetic Resistance ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Cost Effective ◽  
Head Blight ◽  
Fhb Resistance

Durum wheat is an economically important crop for Canadian farmers. Fusarium head blight (FHB) is one of the most destructive diseases that threatens durum production in Canada. FHB reduces yield and end-use quality and most commonly contaminates the grain with the fungal mycotoxin deoxynivalenol, also known as DON. Serious outbreaks of FHB can occur in durum wheat in Canada, and combining genetic resistance with fungicide application is a cost effective approach to control this disease. However, there is limited variation for genetic resistance to FHB in elite Canadian durum cultivars. To explore and identify useful genetic FHB resistance variation for the improvement of Canadian durum wheat, we assembled an association mapping (AM) panel of diverse durum germplasms and performed genome wide association analysis (GWAS). Thirty-one quantitative trait loci (QTL) across all 14 chromosomes were significantly associated with FHB resistance. On 3BS, a stable QTL with a larger effect for resistance was located close to the centromere of 3BS. Three haplotypes of Fhb1 QTL were identified, with an emmer wheat haplotype contributing to disease susceptibility. The large number of QTL identified here can provide a rich resource to improve FHB resistance in commercially grown durum wheat. Among the 31 QTL most were associated with plant height and/or flower time. QTL 1A.1, 1A.2, 3B.2, 5A.1, 6A.1, 7A.3 were associated with FHB resistance and not associated or only weakly associated with flowering time nor plant height. These QTL have features that would make them good targets for FHB resistance breeding.

scholarly journals Mapping of Quantitative Trait Loci for Fusarium Head Blight Resistance in Barley

2000 ◽  
Vol 90 (10) ◽  
pp. 1079-1088 ◽  
Author(s):  
Zhengqiang Ma ◽  
Brian J. Steffenson ◽  
Louis K. Prom ◽  
Nora L. V. Lapitan
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Head Blight ◽  
Plant Height ◽  
Quantitative Trait ◽  
Morphological Traits ◽  
Heading Date ◽  
Rflp Markers ◽  
Head Blight ◽  
Trait Loci ◽  
Fhb Resistance

Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat and barley. Barley grains infected with deoxynivalenol (DON), a vomitoxin produced by Fusarium graminearum, are rejected for malting and brewing. Among six-rowed barley cultivars tested thus far, only cv. Chevron exhibited resistance. This study was conducted to map genes and to identify DNA markers for marker-assisted breeding for FHB resistance in cv. Chevron with restriction fragment length polymorphism (RFLP) markers. A doubled haploid (DH) population was created from a cross between cv. Chevron and susceptible cv. Stander. Seven field experiments were conducted in four different locations in 2 years. A RFLP map containing 211 loci and covering over 1,000 centimorgans (cM) of the genome was used to map quantitative trait loci (QTL) associated with relatively low FHB severity and DON concentration. Morphological traits differing between the parents were also measured: heading date, plant height, spike angle, number of nodes per cm of rachis in the spike, and kernel plumpness. Many of the QTL for FHB and DON coincided with QTLs for these morphological traits. The “fix-QTL” algorithm in Mapmaker QTL was used to remove the part of the variance for FHB resistance that may be explained by heading date or plant height. Results from this study suggest that QTLs with major effects for FHB resistance probably do not exist in cv. Chevron. Three QTL intervals, Xcmwg706-Xbcd441 on chromosome 1H, Xbcd307b-Xcdo684b on chromosome 2H, and Xcdo959b-Xabg472 on chromosome 4H, that are not associated with late heading or height may be useful for marker-assisted selection.

Screening of an EMS mutagenized population of a wheat cultivar susceptible to Fusarium head blight identifies resistant variants

Plant Disease ◽  
2021 ◽  
Author(s):  
Bhavit Chhabra ◽  
Lovepreet Singh ◽  
Sydney Wallace ◽  
Adam Schoen ◽  
Yanhong Dong ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Wheat Cultivar ◽  
Genetic Resistance ◽  
Field Testing ◽  
Head Blight ◽  
Point Of Sale ◽  
Alternative Approaches ◽  
Small Grains ◽  
Fhb Resistance ◽  
Mutant Lines

Fusarium head blight (FHB) primarily caused by Fusarium graminearum is a key disease of small grains. Diseased spikes show symptoms of premature bleaching shortly after infection and have aborted or shriveled seeds, resulting in reduced yields. The fungus also deteriorates quality and safety of the grain due to production of mycotoxins, especially deoxynivalenol (DON), which can result in grain being docked or rejected at the point of sale. Genetic host resistance to FHB is quantitative and no complete genetic resistance against this devastating disease is available. Alternative approaches to develop new sources of FHB resistance are needed. In this study, we performed extensive forward genetic screening of the M4 generation of an EMS induced mutagenized population of cultivar Jagger to isolate variants with FHB resistance. In field testing, 74 mutant lines were found to have resistance against FHB spread and 30 lines out of these also had low DON content. Subsequent testing over two years in controlled greenhouse conditions revealed ten M6 lines showing significantly lower FHB spread. Seven and six lines out of those 10 lines also had reduced DON content and lower FDKs, respectively. Future endeavors will include identification of the mutations that led to resistance in these variants.

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