scholarly journals Functional significance of repressor element 1 silencing transcription factor (REST) target genes in pancreatic beta cells

Diabetologia ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 1429-1439 ◽  
Author(s):  
D. Martin ◽  
F. Allagnat ◽  
G. Chaffard ◽  
D. Caille ◽  
M. Fukuda ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Beta Cells ◽  
Functional Significance ◽  
Pancreatic Beta Cells ◽  
Target Genes ◽  
Repressor Element

scholarly journals Genome-wide analysis of repressor element 1 silencing transcription factor/neuron-restrictive silencing factor (REST/NRSF) target genes

2004 ◽  
Vol 101 (28) ◽  
pp. 10458-10463 ◽  
Author(s):  
A. W. Bruce ◽  
I. J. Donaldson ◽  
I. C. Wood ◽  
S. A. Yerbury ◽  
M. I. Sadowski ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Target Genes ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Repressor Element

Interaction of the Repressor Element 1-silencing Transcription Factor (REST) with Target Genes

2003 ◽  
Vol 334 (5) ◽  
pp. 863-874 ◽  
Author(s):  
Ian C. Wood ◽  
Nikolai D. Belyaev ◽  
Alexander W. Bruce ◽  
Caroline Jones ◽  
Mohini Mistry ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Target Genes ◽  
Repressor Element

scholarly journals The Transcription Factor C/EBP delta Has Anti-Apoptotic and Anti-Inflammatory Roles in Pancreatic Beta Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31062 ◽  
Author(s):  
Fabrice Moore ◽  
Izortze Santin ◽  
Tatiane C. Nogueira ◽  
Esteban N. Gurzov ◽  
Lorella Marselli ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Anti Inflammatory ◽  
Factor C

scholarly journals MicroRNAs Regulate the Wnt/Ca2+ Signaling Pathway to Promote the Secretion of Insulin in Pancreatic Nestin-Positive Progenitor Cells

2014 ◽  
Author(s):  
Chunyu Bai ◽  
Xiangchen Li ◽  
Yuhua Gao ◽  
Taofeng Lu ◽  
Kunfu Wang ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Progenitor Cells ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Target Genes ◽  
Cell Function ◽  
Expression Profiles ◽  
Control Cell ◽  
Small Noncoding Rnas ◽  
And Function

MicroRNAs (miRNAs) are small noncoding RNAs that bind to the 3?-UTR of mRNAs and function mainly in post-transcriptional regulation. MiRNAs have been implicated to play roles in organ development, including that of the pancreas. Many miRNAs, such as miR-375, miR-124, miR-7, miR-21 and miR-221, have been shown to regulate insulin production as well as insulin secretion. However, it is not known whether miRNAs can regulate insulin secretion via the control of intracellular Ca2+ in pancreatic beta cells. In this research, expression profiles of miRNAs and mRNAs were investigated using RNA-sequencing and microarray analysis in chicken pancreatic nestin-positive progenitor cells and differentiated pancreatic beta cells. A number of miRNAs were up-regulated after differentiation of progenitors into beta cells, which regulate cell signaling pathways to control cell function. miR-223 and miR146a were shown to promote insulin secretion from pancreatic beta cells by regulating the concentration of intracellular Ca2+ via the down-regulation of their target genes.

scholarly journals Transcription factor ZBED6 affects gene expression, proliferation, and cell death in pancreatic beta cells

2013 ◽  
Vol 110 (40) ◽  
pp. 15997-16002 ◽  
Author(s):  
X. Wang ◽  
L. Jiang ◽  
O. Wallerman ◽  
U. Engstrom ◽  
A. Ameur ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Transcription Factor ◽  
Beta Cells ◽  
Pancreatic Beta Cells

scholarly journals The MODY1 Gene for Hepatocyte Nuclear Factor 4α and a Feedback Loop Control COUP-TFII Expression in Pancreatic Beta Cells

2008 ◽  
Vol 28 (14) ◽  
pp. 4588-4597 ◽  
Author(s):  
Anaïs Perilhou ◽  
Cécile Tourrel-Cuzin ◽  
Pili Zhang ◽  
Ilham Kharroubi ◽  
Haiyan Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Insulin Secretion ◽  
Binding Site ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Gene Promoter ◽  
Mrna Levels ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 4Α

ABSTRACT Pancreatic islet beta cell differentiation and function are dependent upon a group of transcription factors that maintain the expression of key genes and suppress others. Knockout mice with the heterozygous deletion of the gene for chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) or the complete disruption of the gene for hepatocyte nuclear factor 4α (HNF4α) in pancreatic beta cells have similar insulin secretion defects, leading us to hypothesize that there is transcriptional cross talk between these two nuclear receptors. Here, we demonstrate specific HNF4α activation of a reporter plasmid containing the COUP-TFII gene promoter region in transfected pancreatic beta cells. The stable association of the endogenous HNF4α with a region of the COUP-TFII gene promoter that contains a direct repeat 1 (DR-1) binding site was revealed by chromatin immunoprecipitation. Mutation experiments showed that this DR-1 site is essential for HNF4α transactivation of COUP-TFII. The dominant negative suppression of HNF4α function decreased endogenous COUP-TFII expression, and the specific inactivation of COUP-TFII by small interfering RNA caused HNF4α mRNA levels in 832/13 INS-1 cells to decrease. This positive regulation of HNF4α by COUP-TFII was confirmed by the adenovirus-mediated overexpression of human COUP-TFII (hCOUP-TFII), which increased HNF4α mRNA levels in 832/13 INS-1 cells and in mouse pancreatic islets. Finally, hCOUP-TFII overexpression showed that there is direct COUP-TFII autorepression, as COUP-TFII occupies the proximal DR-1 binding site of its own gene in vivo. Therefore, COUP-TFII may contribute to the control of insulin secretion through the complex HNF4α/maturity-onset diabetes of the young 1 (MODY1) transcription factor network operating in beta cells.

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