Alkaline biodegradable implants for osteoporotic bone defects—importance of microenvironment pH

2015 ◽  
Vol 27 (1) ◽  
pp. 93-104 ◽  
Author(s):  
W. Liu ◽  
T. Wang ◽  
C. Yang ◽  
B. W. Darvell ◽  
J. Wu ◽  
...  
Keyword(s):  
Bone Defects ◽  
Osteoporotic Bone ◽  
Biodegradable Implants ◽  
Microenvironment Ph

scholarly journals Effect of Systemic Zoledronic Acid Dosing Regimens on Bone Regeneration in Osteoporotic Rats

2021 ◽  
Vol 11 (4) ◽  
pp. 1906
Author(s):  
Marwa Y. Shaheen ◽  
Amani M. Basudan ◽  
Abdurahman A. Niazy ◽  
Jeroen J. J. P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Favorable Effect ◽  
Osteoporotic Bone ◽  
Bovine Bone ◽  
Graft Placement ◽  
Regeneration Of Bone

The aim of this study was to evaluate the regeneration of bone defects created in the femoral condyle of osteoporotic rats, following intravenous (IV) zoledronate (ZA) treatment in three settings: pre-bone grafting (ZA-Pre), post-bone grafting (ZA-Post), and pre- plus post-bone grafting (ZA-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, bone defects were created in the left femoral condyle. All defects were grafted with a particulate inorganic cancellous bovine bone substitute. ZA (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-bone graft placement. To another six rats, ZA was given post-bone graft placement creation and continued for 6 weeks. Additional six rats received ZA treatment pre- and post-bone graft placement. Control animals received weekly saline intravenous injections. At 6 weeks post-bone graft placement, samples were retrieved for histological evaluation of the bone area percentage (BA%) and remaining bone graft percentage (RBG%). BA% for ZA-Pre (50.1 ± 3.5%) and ZA-Post (49.2 ± 8.2%) rats was significantly increased compared to that of the controls (35.4 ± 5.4%, p-value 0.031 and 0.043, respectively). In contrast, ZA-Pre+Post rats (40.7 ± 16.0%) showed similar BA% compared to saline controls (p = 0.663). For RBG%, all experimental groups showed similar results ranging from 36.3 to 47.1%. Our data indicate that pre- or post-surgical systemic IV administration of ZA improves the regeneration of bone defects grafted with inorganic cancellous bovine-bone particles in osteoporotic bone conditions. However, no favorable effect on bone repair was seen for continued pre- plus post-surgical ZA treatment.

scholarly journals Repair of Osteoporotic Bone Defects Using Adipose-Derived Stromal Cells and Umbilical Vein Endothelial Cells Seeded in Chitosan/Nanohydroxyapatite-P24 Nanocomposite Scaffolds

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yifei Fang ◽  
Yong Gong ◽  
Zhijian Yang ◽  
Yan Chen
Keyword(s):  
Endothelial Cells ◽  
Bone Defect ◽  
Stromal Cells ◽  
Umbilical Vein ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Adipose Derived Stromal Cells ◽  
Nanocomposite Scaffold ◽  
Umbilical Vein Endothelial Cells ◽  
Nanocomposite Scaffolds

Background. The cell regeneration and blood supply of bone defect lesions are restricted under osteoporotic pathological conditions, which make the healing of bone defect of osteoporosis still a great challenge. The current therapeutic strategies that mainly inhibit bone resorption are not always satisfactory for osteoporotic bone defects, which make the development of new therapies an urgent need. Methods. Previously, we prepared chitosan/nanohydroxyapatite (CS/nHA) biomimetic nanocomposite scaffolds for controlled delivery of bone morphogenetic protein 2-derived peptide (P24). In this study, we determined the effect of coculturing adipose-derived stromal cells (ADSCs) and human umbilical vein endothelial cells (HUVECs) with the CS-P24/nHA nanocomposite scaffolds on osteoporotic bone defect healing. In vitro mixed coculture models were employed to assess the direct effects of coculture. Results. ADSCs cocultured with HUVECs showed significantly greater osteogenic differentiation and mineralization compared with ADSCs or HUVECs alone. The CS-P24/nHA scaffold cocultured with ADSCs and HUVECs was more effective in inducing osteoporotic bone repair, as demonstrated by micro-computed tomography and histology of critical-sized calvariae defects in ovariectomized rats. Calvariae defects treated with the CS-P24/nHA nanocomposite scaffold plus ADSC/HUVEC coculture had a greater area of repair and better reconstitution of osseous structures compared with defects treated with the scaffold plus ADSCs or the scaffold plus HUVECs after 4 and 8 weeks. Conclusion. Taken together, coculture of ADSCs and HUVECs with the CS-P24/nHA nanocomposite scaffold is an effective combination to repair osteoporotic bone defects.

scholarly journals Hybrid particles derived from alendronate and bioactive glass for treatment of osteoporotic bone defects

2019 ◽  
Vol 7 (5) ◽  
pp. 796-808 ◽  
Author(s):  
Mani Diba ◽  
Winston A. Camargo ◽  
Tatiana Zinkevich ◽  
Alina Grünewald ◽  
Rainer Detsch ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Hybrid Particles

Novel hybrid particles are synthesized using alendronate and bioactive glass, which can stimulate regeneration of osteoporotic bone defects.

Conjunction of gallium doping and calcium silicate mediates osteoblastic and osteoclastic performances of tricalcium phosphate bioceramics

2021 ◽  
Author(s):  
Fupo He ◽  
Chao Qiu ◽  
Teliang Lu ◽  
Xuetao Shi ◽  
Jiandong Ye
Keyword(s):  
Mechanical Strength ◽  
Tricalcium Phosphate ◽  
Calcium Silicate ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Densification Process ◽  
Potent Effect ◽  
Inhibition Effect ◽  
Gallium Doping

Abstract Gallium-containing biomaterials are considered promising for reconstructing osteoporotic bone defects, owing to the potent effect of gallium on restraining osteoclast activities. Nevertheless, the gallium-containing biomaterials were demonstrated to disturb the osteoblast activities. In this study, tricalcium phosphate (TCP) bioceramics were modified by gallium doping in conjunction with incorporation of calcium silicate (CS). The results indicated that the incorporation of CS promoted transition of β-TCP to α-TCP, and accelerated densification process, but did not improve the mechanical strength of bioceramics. The silicon released from the composite bioceramics diminished the inhibition effect of released gallium on osteoblast activities, and maintained its effect on restraining osteoclast activities. The TCP-based bioceramics doped with 2.5 mol% gallium and incorporated with 10 mol% CS are considered suitable for treating the bone defects in the osteoporotic environment.

Application of Tantalum-Containing Chitosan Scaffolds for the Repair of Osteoporotic Bone Defects

2018 ◽  
Vol 10 (8) ◽  
pp. 1179-1189
Author(s):  
An Wang ◽  
Wenbo Lin ◽  
Jun Ma ◽  
Liangyu Shi ◽  
Weiheng Wang ◽  
...  
Keyword(s):  
Bone Defects ◽  
Osteoporotic Bone ◽  
Chitosan Scaffolds

scholarly journals Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Bo Wei ◽  
Chengshuo Huang ◽  
Mingyan Zhao ◽  
Peng Li ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Platelet Rich Plasma ◽  
Bone Microarchitecture ◽  
Bone Defects ◽  
Ovariectomized Rats ◽  
Marker Genes ◽  
Osteoporotic Bone ◽  
Specific Marker ◽  
Allogeneic Bone

We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results. We also assessed changes to bone trabeculae in the proximal tibial growth plate. In ovariectomized rats treated with PRP or with a combination of PRP and BMSCs, the trabecular connectivity densities (Conn.D), bone volume ratios (BV/TV), and numbers (Tb.N) in the defect areas increased significantly from day 7 to day 42. These results indicate that PRP treatment enhances bone microarchitecture in osteoporosis. Moreover, expression levels of osteogenesis-specific marker genes including RUNX2, OSX, and OPN were significantly upregulated in rats treated with PRP and BMSCs compared to those of other groups. Thus, we conclude that treatment with PRP combined with BMSCs significantly promotes healing of osteoporotic bone defects. This study provides an alternative strategy for the treatment of osteoporotic bone loss.

Healing of osteoporotic bone defects by baculovirus-engineered bone marrow-derived MSCs expressing MicroRNA sponges

Biomaterials ◽  
2016 ◽  
Vol 74 ◽  
pp. 155-166 ◽  
Author(s):  
Kuei-Chang Li ◽  
Yu-Han Chang ◽  
Chia-Lin Yeh ◽  
Yu-Chen Hu
Keyword(s):  
Bone Marrow ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Microrna Sponges

scholarly journals Baculovirus-Mediated miR-214 Knockdown Shifts Osteoporotic ASCs Differentiation and Improves Osteoporotic Bone Defects Repair

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kuei-Chang Li ◽  
Yu-Han Chang ◽  
Mu-Nung Hsu ◽  
Shih-Chun Lo ◽  
Wan-Hua Li ◽  
...  
Keyword(s):  
Bone Defects ◽  
Osteoporotic Bone
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