Lectin genes in the Frankia alni genome

Plant proteins with lectin domains play an essential role in plant immunity modulation, but among a plurality of lectins recruited by plants, only a few members have been functionally characterized. For the analysis of flax lectin gene expression, we used FIBexDB, which includes an efficient algorithm for flax gene expression analysis combining gene clustering and coexpression network analysis. We analyzed the lectin gene expression in various flax tissues, including root tips infected with Fusarium oxysporum. Two pools of lectin genes were revealed: downregulated and upregulated during the infection. Lectins with suppressed gene expression are associated with protein biosynthesis (Calreticulin family), cell wall biosynthesis (galactose-binding lectin family) and cytoskeleton functioning (Malectin family). Among the upregulated lectin genes were those encoding lectins from the Hevein, Nictaba, and GNA families. The main participants from each group are discussed. A list of lectin genes, the expression of which can determine the resistance of flax, is proposed, for example, the genes encoding amaranthins. We demonstrate that FIBexDB is an efficient tool both for the visualization of data, and for searching for the general patterns of lectin genes that may play an essential role in normal plant development and defense.

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Early transcriptomic response of Alnus glutinosa to Frankia alni symbiont, an upregulated nsLTP (non-specific Lipid Transfer Protein) is implicated in early and late stages of symbiosis

10.1101/2021.10.29.465983 ◽

2021 ◽

Author(s):

Melanie Gasser ◽

Nicole Alloisio ◽

Pascale Fournier ◽

Severine Balmand ◽

Ons Kharrat ◽

...

Keyword(s):

Root Hair ◽

Lipid Transfer Protein ◽

Alnus Glutinosa ◽

Lipid Transfer ◽

Transcriptomic Response ◽

Transfer Protein ◽

Root Hair Deformation ◽

Frankia Alni ◽

Late Stages ◽

Specific Lipid

The response of Alnus glutinosa to Frankia alni is complex with several sequential physiological modifications that include calcium spiking, root hair deformation, penetration, induction of primordium, formation and growth of nodule. A transcriptomic study of seedlings in hydroponics after early contact (2.5 days) with Frankia alni, either with a culture supernatant or with living cells separated from the roots by a dialysis membrane, permitted to identify plant genes which expression level was modified upon early contact with Frankia. Forty-two genes were significantly up-regulated in both experiments, most of them encoding biological processes such as oxidative stress or response to stimuli. Among them, the most upregulated gene was a non-specific lipid transfer protein encoding gene with a fold change of 141. This nsLTP was found to increase Frankia nitrogen fixation at sub-lethal concentration. Interestingly, it was immunolocalized to a region of the deformed root hair at an early infection stage and later in nodules, it was localized around bacterial vesicles suggesting a role in early and late stages of symbiosis.

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THE STRUCTURE OF SOYBEAN LECTIN GENES AND AN INSERTION SEQUENCE

Advances in Gene Technology: Molecular Genetics of Plants and Animals ◽

10.1016/b978-0-12-221480-6.50089-x ◽

1983 ◽

pp. 595

Author(s):

P.R. Rhodes ◽

L.O. Vodkin ◽

R.B. Goldberg

Keyword(s):

Insertion Sequence ◽

Lectin Genes ◽

Soybean Lectin

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Combined Transcriptome Profiling Reveals a Novel Family of Arbuscular Mycorrhizal-Specific Medicago truncatula Lectin Genes

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-18-0771 ◽

2005 ◽

Vol 18 (8) ◽

pp. 771-782 ◽

Cited By ~ 52

Author(s):

André Frenzel ◽

Katja Manthey ◽

Andreas M. Perlick ◽

Folker Meyer ◽

Alfred Pühler ◽

...

Keyword(s):

Cdna Library ◽

Medicago Truncatula ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Host Cells ◽

Genomic Research ◽

Cdna Libraries ◽

Specific Expression ◽

Transgenic Roots ◽

Lectin Genes

The large majority of plants are capable of undergoing a tight symbiosis with arbuscular mycorrhizal (AM) fungi. During this symbiosis, highly specialized new structures called arbuscules are formed within the host cells, indicating that, during interaction with AM fungi, plants express AM-specific genetic programs. Despite increasing efforts, the number of genes known to be induced in the AM symbiosis is still low. In order to identify novel AM-induced genes which have not been listed before, 5,646 expressed sequence tags (ESTs) were generated from two Medicago truncatula cDNA libraries: a random cDNA library (MtAmp) and a suppression subtractive hybridization (SSH) library (MtGim), the latter being designed to enhance the cloning of mycorrhiza-upregulated genes. In silico expression analysis was applied to identify those tentative consensus sequences (TCs) of The Institute for Genomic Research M. truncatula gene index (MtGI) that are composed exclusively of ESTs deriving from the MtGim or MtAmp library, but not from any other cDNA library of the MtGI. This search revealed 115 MtAmp- or MTGim-specific TCs. For the majority of these TCs with sequence similarities to plant genes, the AM-specific expression was verified by quantitative reverse-transcription polymerase chain reaction. Annotation of the novel genes induced in mycorrhizal roots suggested their involvement in different transport as well as signaling processes and revealed a novel family of AM-specific lectin genes. The expression of reporter gene fusions in transgenic roots revealed an arbuscule-related expression of two members of the lectin gene family, indicating a role for AM-specific lectins during arbuscule formation or functioning.

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