Development of sampling approaches for the determination of the presence of genetically modified organisms at the field level

2010 ◽  
Vol 396 (6) ◽  
pp. 2031-2041 ◽  
Author(s):  
Jelka Šuštar-Vozlič ◽  
Katja Rostohar ◽  
Andrej Blejec ◽  
Petra Kozjak ◽  
Zoran Čergan ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Field Level

Determination of Knowledge, Attitude, Behavior about Genetically Modified Organisms in Nursing School Students

2013 ◽  
Vol 55 (4) ◽  
pp. 297 ◽  
Author(s):  
turker turker ◽  
necmettin kocak ◽  
Ibrahim Aydin ◽  
hakan istanbulluoglu ◽  
nuri yildiran ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Nursing School ◽  
School Students ◽  
Attitude Behavior

scholarly journals Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
A. Burrell ◽  
C. Foy ◽  
M. Burns
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Fitness For Purpose ◽  
Chain Reaction ◽  
Food Authenticity ◽  
Pcr Products ◽  
Polymerase Chain

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.

scholarly journals Challenges for the characterization of genetically modified animals by the qPCR technique in the era of genomic editing

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Ribrio Ivan Tavares Pereira Batista ◽  
Dárcio Ítalo Alves Teixeira ◽  
Vicente José de Figueirêdo Freitas ◽  
Luciana Magalhães Melo ◽  
Joanna Maria Gonçalves Souza-Fabjan
Keyword(s):  
Copy Number ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Transgenic Animals ◽  
Real Time Quantitative Pcr ◽  
Transgenic Organisms ◽  
Pcr Technology ◽  
Genomic Editing

Characterization of genetically modified organisms through determination of zygosity and transgene integration concerning both copy number and genome site is important for breeding a transgenic line and the use of these organisms in the purpose for which it was obtained. Southern-blot, fluorescence in situ hybridization or mating are demanding and time-consuming techniques traditionally used in the characterization of transgenic organisms and, with the exception of mating, give ambiguous results. With the emergence of the real-time quantitative PCR technology, different applications have been described for the analysis of transgenic organisms by determination of several parameters to transgenic analysis. However, the accuracy in quantitation by this method can be influenced in all steps of quantification. This review focuses on the aspects that influence pre-analytical steps (DNA extraction and DNA quantification methods), quantification strategies and data analysis in quantification of copy number and zygosity in transgenic animals.

High-Throughput Double Quantitative Competitive Polymerase Chain Reaction for Determination of Genetically Modified Organisms

2005 ◽  
Vol 77 (15) ◽  
pp. 4785-4791 ◽  
Author(s):  
Anastasia K. Mavropoulou ◽  
Theodora Koraki ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

2002 ◽  
Vol 214 (1) ◽  
pp. 3-26 ◽  
Author(s):  
Elke Anklam ◽  
Ferruccio Gadani ◽  
Petra Heinze ◽  
Hans Pijnenburg ◽  
Guy Van Den Eede
Keyword(s):  
Analytical Methods ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Agricultural Crops

scholarly journals Knowledge of adolescents completing secondary schools concerning genetically modified organisms (GMO)

2016 ◽  
Vol 27 (2) ◽  
pp. 38-43 ◽  
Author(s):  
Magdalena Florek-Łuszczki ◽  
Stanisław Lachowski ◽  
Jarosław Chmielewski ◽  
Anna Jurkiewicz
Keyword(s):  
Secondary Schools ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Genetically Modified Organism ◽  
Technical Schools ◽  
Demographic Traits ◽  
Level Of Knowledge ◽  
The Relationship ◽  
Type Of School

Abstract The objective of the conducted analyses is the evaluation of the level of knowledge concerning the scope of problems related with genetically modified organism (GMO) amongst adolescents completing secondary schools and the determination of the relationship between the level of this knowledge and the selected demographic traits of the adolescents examined.The scope of problems undertaken was elaborated based on the survey conducted in a group of 500 adolescents from the Lublin Region completing secondary schools, including 250 adolescents attending General Secondary Schools and 250 adolescents attending Agricultural Secondary Technical Schools. The study was conducted by the method of a diagnostic survey, using a questionnaire.The study showed that the majority of adolescents completing secondary schools were not interested at all in the scope of problems concerning GMO. A large part of the respondents (more than 2/5) had a very low level of knowledge of this problem. The greatest differences in the level of knowledge about GMO were observed in subgroups divided according to the type of school attended by the adolescents (General or Agricultural Technical). Respondents who attended General Secondary Schools showed a better knowledge of theoretical problems, whereas those who attended Secondary Agricultural Technical Schools were better familiarised with practical issues.

scholarly journals КОНТРОЛЬ ГЕНЕТИЧНО МОДИФІКОВАНИХ РОСЛИН – ГАРАНТІЯ ПРОДОВОЛЬЧОЇ БЕЗПЕКИ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 167-169
Author(s):  
G.V. Kushnir
Keyword(s):  
Genetic Engineering ◽  
Plant Material ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Agricultural Industry ◽  
Food And Feed ◽  
Environmentally Safe ◽  
Potential Risks ◽  
Mechanisms Of Development

One of the problems in formation of mechanisms of development of environmentally safe environment is the proliferation of genetically modified organisms (GMOs). The use of genetic engineering in agriculture, food and agricultural industry have created the new opportunities for manufacturing the food and feed for meet the ever growing needs of the world's population. However, the opinions of scientists about the benefits and risks of genetic engineering is different. So today is particularly acute issue unpredictable consequences and danger for human health and the environment which are connected with GMO.The article present the results of determination of genetically modified organisms in plant material for the period 2013 – 2015 years, from the farms of different ownership forms. Analysis of the conducted research indicates on the circulation in farms of Lviv region transgenic plants.Found that the most common genetically modified plants were maize, soya and rape. The research of plant material on the presence of GMOs was performed by polymerase chain reaction in real time (PCR RF) by setting in them target sequences of promoter 35S virus of cauliflower mosaic (CaMV), promoter of FMV and (or) terminator NOS (T–NOS) T1 plasmid of Agrobacterium tumefaciens, Pat, EPSPS, Cry 3A, and determined species identification of positive samples. The use of PCR–RF allows quickly and qualitatively detect the GMO in the research samples. In 2013, the percentage of positive samples was 3.9% of the total, in 2014 – 2.3% and in 2015 – 3.3%. It is therefore necessary to monitor the vegetable raw and seed material on the presence of genetically modified organisms, to monitor the situation on GMO in Ukraine, because the problem of biosafety and the evaluation of the potential risks from their use is not studied. 

scholarly journals Використання молекулярно-біологічних методів досліджень у прикладних галузях ветеринарних наук

2016 ◽  
Vol 19 (77) ◽  
pp. 166-169
Author(s):  
B. Nazar ◽  
S. Murska ◽  
D. Gufrij ◽  
B. Gutyj
Keyword(s):  
Antibiotic Resistance ◽  
Infectious Diseases ◽  
Research Methods ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Feed Additives ◽  
Farm Animals ◽  
Biological Research ◽  
Clinical Strains

The article deals with the information on the use of small amounts of molecular research methods in applied fields of veterinary science. Is also considered the main approaches and directions in the sector work of molecular biological research GNIKI of veterinary medicines and feed additives. The objects of researches of molecular biology are specific macromolecule – especially proteins and nucleic acids, the simplest form of living – isolated cell nucleus, organelles of cells, chromosomes, viruses and bacteria. The use of gene technology to analyze food quality for people and feeding stuffs for animal is due to the need for sensitive, rapid and accurate method. Using the PCR method is common for indication and identification of the causative agents of food poisoning. Based on the PCR it was developed methods for analyzing the products, which were made for the use of transgenic microorganisms. The actual use of the PCR is the determination of clinical strains antibiotic resistance of infectious diseases, creating the possibility of quantifying results account to control the dynamics infectious process, the correct choice of treatment and evaluating the effectiveness of used medicines. An important direction of the application of DNA technology is to identify products obtained after using genetic engineering techniques. Thereby, by means of molecular biological research methods the sector of molecular biological studies can perform the following tasks: detection, identification and quantification of the content of genetically modified organisms; identification of plant species of animal protein in premixes, fodder additives and animal feed; conducting monitoring investigations the content of genetically modified organisms and ruminant DNA in premixes, feed additives and animal nutrition according to an annual monitoring plan of feed, feed additives and premixes; in the future diagnosing infectious (bacteria) and parasitic exciter in farm animals and determination of antibiotic resistance in clinical strains of infectious diseases.

scholarly journals Genetically modified organisms (GMO) in opinions completing secondary schools in Lublin

2016 ◽  
Vol 27 (3) ◽  
pp. 24-29
Author(s):  
Stanisław Lachowski ◽  
Magdalena Florek-Łuszczki ◽  
Anna Jurkiewicz ◽  
Jarosław Chmielewski ◽  
Ilona Żeber-Dzikowska
Keyword(s):  
Secondary Schools ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Technical Schools ◽  
Level Of Knowledge ◽  
The Relationship ◽  
General Profile

AbstractThe objective of the conducted analysis is the opinion of adolescents completing secondary schools concerning genetically modified organisms (GMO) and determination of the relationship between the level of knowledge concerning GMO, and evaluation of the safety of their use in industry and agriculture.The scope of problems undertaken was elaborated based on the survey studies conducted on a group of 500 adolescents completing secondary schools from the Lublin Region, including 250 attending general profile secondary schools and 250 from agricultural technical schools. The study was conducted by the method of a diagnostic survey, using the questionnaire technique. The results of the study showed that the respondents perceived both positive and negative aspects of producing genetically modified (GM) products. These evaluations were significantly related with the respondents’ level of knowledge concerning GMO. The higher the level of knowledge, the more positive the evaluations of the adolescents concerning the cultivation and breeding of GMO, and more frequent indications that the consumption of products manufactured from these organisms is safe.

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