Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

2002 ◽  
Vol 214 (1) ◽  
pp. 3-26 ◽  
Author(s):  
Elke Anklam ◽  
Ferruccio Gadani ◽  
Petra Heinze ◽  
Hans Pijnenburg ◽  
Guy Van Den Eede
Keyword(s):  
Analytical Methods ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Agricultural Crops

Determination of Knowledge, Attitude, Behavior about Genetically Modified Organisms in Nursing School Students

2013 ◽  
Vol 55 (4) ◽  
pp. 297 ◽  
Author(s):  
turker turker ◽  
necmettin kocak ◽  
Ibrahim Aydin ◽  
hakan istanbulluoglu ◽  
nuri yildiran ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Nursing School ◽  
School Students ◽  
Attitude Behavior

Development of sampling approaches for the determination of the presence of genetically modified organisms at the field level

2010 ◽  
Vol 396 (6) ◽  
pp. 2031-2041 ◽  
Author(s):  
Jelka Šuštar-Vozlič ◽  
Katja Rostohar ◽  
Andrej Blejec ◽  
Petra Kozjak ◽  
Zoran Čergan ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Field Level

scholarly journals Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products

2020 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Eleni Spanea ◽  
Theofania Tsironi ◽  
Efstathia Tsakali ◽  
Anthimia Batrinou ◽  
Valentina Stefanou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Assay Method ◽  
Genetically Modified Maize ◽  
Nos Terminator ◽  
Repeatability And Reproducibility ◽  
Pcr Method

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GMΟ detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

scholarly journals Detection of Genetically Modified Protein in Soy-containing Foods

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Trace Agostino ◽  
Amanda Trukus ◽  
Micheal Jain
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Grocery Store ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Consumption ◽  
Specific Interest ◽  
Epsp Synthase ◽  
Genetic Modifications ◽  
Contamination Levels

The use of genetically modified organisms (GMO) in food is a highly public and controversial issue. We have used an enzyme-linked immunosorbent assay (ELISA) to detect the genetically modified protein 5-enol-pyruvylshikimate-3-phosphate (EPSP) synthase within soy-containing food products from the grocery store. We found that EPSP synthase is detectable in 3 out of 5 food products tested. Of specific interest, we found contamination levels of EPSP synthase (0.36%) in Heinz' Pablum Soya Cereal, which is currently deemed to be free of genetic modifications by the company as well as by Greenpeace Canada. These results demonstrate that genetically modified organisms are present in foods commonly available for human consumption and that the widespread use of this technology may make it difficult to ensure that any given product is free of all traces of genetically modified protein.

scholarly journals Consumer perception and preference towards genetically modified (GM) foods: bibliometric evidence and policy imperatives.

2021 ◽  
Author(s):  
R. Sendhil ◽  
Joan Nyika ◽  
Sheel Yadav ◽  
Joby Mackolil ◽  
Prashat G. Rama Prashat ◽  
...  
Keyword(s):  
Genetically Modified Organisms ◽  
Food System ◽  
Research Output ◽  
Genetically Modified ◽  
Public Awareness ◽  
Crop Improvement ◽  
Food Products ◽  
Consumer Perception ◽  
Gm Food ◽  
Gm Foods

Abstract Background: Genetically modified (GM) foods could be a promising option to reduce the dependence of the agri-food system on conventional means of crop improvement. However, the safety of GM food remains a concern, in addition to the perceived and real risks to environment. The opinion about scientific evidence among researchers, policymakers and government regulators on promotion of GM foods for consumers differ. This study presents the trends emerging from research outputs on consumer perception and preference for GM foods and policy prescriptions for enabling the consumption. Scope and Approach: The study mainly focused on (1) exploring the publication trends, geographic focus of research and a comprehensive review on the consumers' perception of and preference to GM foods, (2) identifying research hotspots and emerging genetically modified organisms (GMO) research themes, (3) assessing the integrated nature of GMO research, and (4) suggesting policy prescriptions on challenges and way forward. Bibliometric analyses have been performed using the Web of Science (WOS) database sourcing literature from 1981 to 2021 and analyzed the final sample of 543 publications using BibExcel and VOSviewer visualization software tools. The search strategy utilized the topic basis search of phrases related to GM food preference and perception. Key Findings and Conclusions: The search query retrieving 543 journal articles showed an increasing trend in publications on consumer's response towards GM foods. Thematic analysis revealed a strong interlinkage of GMOs research with 'agriculture' and 'food science technology'. Consumers' positive response is largely influenced by the decision of the governments to ban or approve the GM crops cultivation. Similarly, the public support increases when the potential benefits of the technology are well articulated, consumption increases with a price discount, people's trust on the government and belief in science increases with a positive influence by the media. Mapping of the retrieved articles showed the top four recurrent keywords: GMOs, biotechnology, attitudes and acceptance. Europe and the USA are the first region and country, respectively, in terms of the number of active institutions per research output, per capita GDP publication and citations per article. The National Natural Science Foundation of China, followed by the National University of Malaysia are the top fundraisers for research on GM food consumption, perceptions and attitudes. Although research outputs on GM food consumer's acceptance and preferences are increasing, a majority of publications came from developed nations. Thus, intensive research is yet to be carried out in developing countries to produce a genetically modified food product with superior properties and substantial equivalence with no significant side-effects. We suggest research-, agri-food industries-, and society-oriented policies needed to be implemented by the stakeholders to ensure the safety of GM food products, encourage consumer-based studies, and increase public awareness towards these food products.

scholarly journals Educated Millennials and Credence Attributes of Food Products with Genetically Modified Organisms: Knowledge, Trust and Social Media

2020 ◽  
Vol 12 (20) ◽  
pp. 8534 ◽  
Author(s):  
Carlo Russo ◽  
Mariarosaria Simeone ◽  
Maria Angela Perito
Keyword(s):  
Social Media ◽  
Scientific Knowledge ◽  
Genetically Modified Organisms ◽  
Ethical Issues ◽  
Genetically Modified ◽  
Food Products ◽  
Health Claims ◽  
Credence Attributes ◽  
Use Of Social Media ◽  
Information Providers

In this paper, we investigated educated millennials’ evaluation of credence attributes in food products containing genetically modified organisms (GMO products). Our goal is to assess whether beliefs about GMO products are determined by scientific knowledge alone or if they are affected by other factors such as trust in information providers and use of social media. The focus on millennials is motivated by the increasing relevance of this social group in the public debate and by their extensive use of social media. We surveyed a sample of 215 Italian college students, confronting them with questions about safety, environmental impact and ethical issues in GMO product consumption. Using an ordered probit regression model, we found that educated millennials build their beliefs using a mix of scientific knowledge and trust in information providers. The role of the two drivers depended on the issue considered. Scientific knowledge drove beliefs in health claims, while trust in information providers was a driving factor in almost all claims. After controlling for trust effects, we did not find evidence of impact of confidence in the reliability of traditional and social media on beliefs. This result contradicts previous literature.

scholarly journals Current PCR Methods for the Detection, Identification and Quantification of Genetically Modified Organisms(GMOs): a Brief Review

2000 ◽  
Vol 19 (2) ◽  
pp. 85-96 ◽  
Author(s):  
F Gadani ◽  
G Bindler ◽  
H Pijenburg ◽  
L Rossi ◽  
J Zuber
Keyword(s):  
Dna Sequences ◽  
Method Development ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Value Added ◽  
Detection Methods ◽  
Agricultural Crops ◽  
The European Union ◽  
Gmo Detection ◽  
Food Ingredients

AbstractAnalytical methods based on the polymerase chain reaction (PCR) technology are increasingly used for the detection of deoxyribonucleic acid (DNA) sequences associated with genetically modified organisms (GMOs). In the European Union and Switzerland, mandatory labeling of novel foods and food ingredients consisting of, or containing GMOs is required according to food regulations and is triggered by the presence of newly introduced foreign DNA sequences, or newly expressed proteins. In order to meet regulatory and consumer demand, numerous PCR-based methods have been developed which can detect, identify and quantify GMOs in agricultural crops, food and feed. Moreover, the determination of genetic identity allows for segregation and traceability (identity preservation) throughout the supply chain of GM crops that have been enhanced with value-added quality traits. Prerequisites for GMO detection include a minimum amount of the target gene and prior knowledge of the type of genetic modification, such as virus or insect resistance traits, including controlling elements (promoters and terminators). Moreover, DNA extraction and purification is a critical step for the preparation of PCR-quality samples, particularly for processed agricultural crops such as tobacco. This paper reviews the state-of-the-art of PCR-based method development for the qualitative and quantitative determination and identification of GMOs, and includes a short summary of official and validated GMO detection methods.

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