High-speed homogenization coupled with microwave-assisted extraction followed by liquid chromatography–tandem mass spectrometry for the direct determination of alkaloids and flavonoids in fresh Isatis tinctoria L. hairy root cultures

2015 ◽  
Vol 407 (16) ◽  
pp. 4841-4848 ◽  
Author(s):  
Jiao Jiao ◽  
Qing-Yan Gai ◽  
Lin Zhang ◽  
Wei Wang ◽  
Meng Luo ◽  
...  
Keyword(s):  
High Speed ◽  
Direct Determination ◽  
Tandem Mass ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Microwave Assisted ◽  
Chromatography Tandem Mass Spectrometry ◽  
Assisted Extraction ◽  
Liquid Chromatography Tandem Mass

Direct determination of astragalosides and isoflavonoids from fresh Astragalus membranaceus hairy root cultures by high speed homogenization coupled with cavitation-accelerated extraction followed by liquid chromatography-tandem mass spectrometry

RSC Advances ◽  
2015 ◽  
Vol 5 (44) ◽  
pp. 34672-34681 ◽  
Author(s):  
Jiao Jiao ◽  
Qing-Yan Gai ◽  
Meng Luo ◽  
Xiao Peng ◽  
Chun-Jian Zhao ◽  
...  
Keyword(s):  
High Speed ◽  
Direct Determination ◽  
Metabolic Profiles ◽  
Tandem Mass ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

HSH-CAE-LC-MS/MS opened up a new avenue for the direct determination of secondary metabolic profiles from fresh plant in vitro cultures.

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