In vitro assessment of pediococci- and lactobacilli-induced cholesterol-lowering effect using digitally enhanced high-performance thin-layer chromatography and confocal microscopy

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

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Effect-directed profiling and identification of bioactive metabolites from field, in vitro-grown and acclimatized Musa spp. accessions using high-performance thin-layer chromatography-mass spectrometry

Journal of Chromatography A ◽

10.1016/j.chroma.2019.460774 ◽

2020 ◽

Vol 1616 ◽

pp. 460774 ◽

Cited By ~ 2

Author(s):

Ibukun O. Ayoola-Oresanya ◽

Mubo A. Sonibare ◽

Badara Gueye ◽

Rajneesh Paliwal ◽

Michael T. Abberton ◽

...

Keyword(s):

Mass Spectrometry ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Bioactive Metabolites ◽

Musa Spp ◽

Chromatography Mass Spectrometry ◽

Layer Chromatography

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High performance thin-layer chromatography and in vitro cytotoxic studies on ethanol extract of Matricaria chamomilla L (Asteraceae) flowers

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i9.27 ◽

2021 ◽

Vol 18 (9) ◽

pp. 1969-1976

Author(s):

Mohammed Al Bratty ◽

Lalitha K. Govindaram ◽

Neelaveni Thangavel ◽

Hassan A. Alhazmi ◽

...

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Mtt Assay ◽

High Performance ◽

Cytotoxic Effect ◽

Ethanol Extract ◽

Matricaria Chamomilla ◽

Layer Chromatography ◽

Mcf 7

Purpose: To develop a high performance thin-layer chromatography (HPTLC procedure for quantitation of apigenin in ethanol extract of Matricaria chamomilla (Babunaj) flowers, and to evaluate the extract for in vitro cytotoxic effect on MCF-7 cell lines. Methods: Quantification of apigenin was carried out using a CAMAG TLC system. A combination of toluene, ethyl acetate and formic acid (4.5:3.5:0.2 v/v/v) was used as mobile phase, with densitometry detection at 336 nm. The HPTLC procedure was subjected to validation as per ICH guidelines. The cytotoxicity of the extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: A sharp apigenin band at Rf of 0.51 was obtained, and the content of apigenin in the extract was 0.062 % w/w. The detection limit (LOD) and quantification limit (LOQ) were 0.19 and 0.57 ng/band, respectively. MTT assay results indicate that M. chamomilla was cytotoxic to Michigan Cancer Foundation-7 (MCF-7) cells, with half-maximal concentration (IC50) of 74 µg/mL. Conclusion: The developed HPTLC method is linear, precise, accurate and specific for the determination of apigenin. M. chamomilla exerts cytotoxic effect on MCF-7 cell line via induction of apoptosis.

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