Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for false-positive 35S-promoter screening results

Abstract Labeling of genetically modified organisms (GMOs) is now in place in many countries, including the European Union, in order to guarantee the consumer's choice between GM and non-GM products. Screening of samples is performed by polymerase chain reaction (PCR) amplification of regulatory sequences frequently introduced into genetically modified plants. Primers for the 35S promoter from Cauliflower mosaic virus (CaMV) are those most frequently used. In virus-infected plants or in samples contaminated with plant material carrying the virus, false-positive results can consequently occur. A system for real-time PCR using a TaqMan minor groove binder probe was designed that allows recognition of virus coat protein in the sample, thus allowing differentiation between transgenic and virus-infected samples. We measured the efficiency of PCR amplification, limits of detection and quantification, range of linearity, and repeatability of the assay in order to assess the applicability of the assay for routine analysis. The specificity of the detection system was tested on various virus isolates and plant species. All 8 CaMV isolates were successfully amplified using the designed system. No cross-reactivity was detected with DNA from 3 isolates of the closely related Carnation etched ring virus. Primers do not amplify plant DNA from available genetically modified maize and soybean lines or from different species of Brassicaceae or Solanaceae that are natural hosts for CaMV. We evaluated the assay for different food matrixes by spiking CaMV DNA into DNA from food samples and have successfully amplified CaMV from all samples. The assay was tested on rapeseed samples from routine GMO testing that were positive in the 35S screening assay, and the presence of the virus was confirmed.

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Detection of cauliflower mosaic virus (CaMV) in single aphids by the polymerase chain reaction (PCR)

Journal of Virological Methods ◽

10.1016/0166-0934(92)90040-k ◽

1992 ◽

Vol 37 (2) ◽

pp. 129-137 ◽

Cited By ~ 22

Author(s):

J.J. López-Moya ◽

J. Cubero ◽

D. López-Abella ◽

J.R. Díaz-Ruíz

Keyword(s):

Polymerase Chain Reaction ◽

Mosaic Virus ◽

Cauliflower Mosaic Virus ◽

Chain Reaction ◽

Polymerase Chain

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Rapid-Antigen Detection Tests for Group A Streptococcal Pharyngitis: Revisiting False-Positive Results Using Polymerase Chain Reaction Testing

The Journal of Pediatrics ◽

10.1016/j.jpeds.2013.01.050 ◽

2013 ◽

Vol 162 (6) ◽

pp. 1282-1284.e1 ◽

Cited By ~ 18

Author(s):

Jérémie F. Cohen ◽

Robert Cohen ◽

Philippe Bidet ◽

Corinne Levy ◽

Patrice Deberdt ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

False Positive ◽

Streptococcal Pharyngitis ◽

Antigen Detection ◽

Chain Reaction ◽

Polymerase Chain Reaction Testing ◽

Group A ◽

Polymerase Chain ◽

Positive Results

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Impact of Rapid Methicillin-Resistant Staphylococcus aureus Polymerase Chain Reaction Testing on Mortality and Cost Effectiveness in Hospitalized Patients with Bacteraemia

PharmacoEconomics ◽

10.2165/11533020-000000000-00000 ◽

2010 ◽

Vol 28 (7) ◽

pp. 567-575 ◽

Cited By ~ 47

Author(s):

Jack Brown ◽

Joseph A. Paladino

Keyword(s):

Staphylococcus Aureus ◽

Polymerase Chain Reaction ◽

Cost Effectiveness ◽

Methicillin Resistant Staphylococcus Aureus ◽

Hospitalized Patients ◽

Chain Reaction ◽

Methicillin Resistant ◽

Polymerase Chain Reaction Testing ◽

Polymerase Chain

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Persistent Leptospiruria in Five Dogs Despite Antimicrobial Treatment (2000–2017)

Journal of the American Animal Hospital Association ◽

10.5326/jaaha-ms-6882 ◽

2019 ◽

Vol 55 (1) ◽

pp. 42-47 ◽

Cited By ~ 3

Author(s):

Tara Mauro ◽

Kenneth Harkin

Keyword(s):

At Risk ◽

Polymerase Chain Reaction ◽

Antimicrobial Treatment ◽

Renal Tubules ◽

Chain Reaction ◽

Polymerase Chain Reaction Testing ◽

Pet Owners ◽

Zoonotic Risk ◽

The Face ◽

Polymerase Chain

ABSTRACT In dogs with leptospirosis, doxycycline therapy is recommended as the preferred therapy for its ability to eliminate the organism from all tissues, including the renal tubules. Elimination of organisms from the renal tubules terminates leptospiruria and prevents transmission of the organism. This report describes the discovery of persistent leptospiruria in the face of therapy with doxycycline in four dogs and enrofloxacin in one dog. Leptospiruria was confirmed by polymerase chain reaction testing for pathogenic leptospires in all five dogs. In two dogs, leptospiruria resolved after a change in therapy to enrofloxacin. In three dogs, doxycycline and/or enrofloxacin were ineffective at eliminating leptospiruria, which then resolved after therapy with clarithromycin. Pet owners could be at risk as persistent leptospiruria poses a potential zoonotic risk. The potential reasons for persistent leptospiruria as demonstrated by polymerase chain reaction testing are discussed.

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