The role of G-domain orientation and nucleotide state on the Ras isoform-specific membrane interaction

2012 ◽  
Vol 41 (10) ◽  
pp. 801-813 ◽  
Author(s):  
Shobhna Kapoor ◽  
Katrin Weise ◽  
Mirko Erlkamp ◽  
Gemma Triola ◽  
Herbert Waldmann ◽  
...  
Keyword(s):  
Membrane Interaction ◽  
Domain Orientation ◽  
Specific Membrane

Biophysical insights into the membrane interaction of the core amyloid-forming Aβ40fragment K16–K28 and its role in the pathogenesis of Alzheimer's disease

2016 ◽  
Vol 18 (25) ◽  
pp. 16890-16901 ◽  
Author(s):  
Swapna Bera ◽  
Kyle J. Korshavn ◽  
Rajiv K. Kar ◽  
Mi Hee Lim ◽  
Ayyalusamy Ramamoorthy ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Hydrophobic Region ◽  
Membrane Interaction ◽  
The Core

Role of central hydrophobic region of Aβ40 in membrane interaction.

The Role of Prostate Specific Membrane Antigen and Pepsinogen C Tissue Expression as an Adjunctive Method to Prostate Cancer Diagnosis

2009 ◽  
Vol 181 (2) ◽  
pp. 594-600 ◽  
Author(s):  
Alberto A. Antunes ◽  
Kátia R. Leite ◽  
Juliana M. Sousa-Canavez ◽  
Luiz H. Camara-Lopes ◽  
Miguel Srougi
Keyword(s):  
Prostate Cancer ◽  
Cancer Diagnosis ◽  
Tissue Expression ◽  
Prostate Specific Membrane Antigen ◽  
Prostate Cancer Diagnosis ◽  
Pepsinogen C ◽  
Specific Membrane

Norepinephrine uptake as an indicator of cardiac reinnervation in dogs

1978 ◽  
Vol 235 (3) ◽  
pp. H289-H294 ◽  
Author(s):  
M. P. Kaye ◽  
G. M. Tyce
Keyword(s):  
Electrical Stimulation ◽  
Sympathetic Nerves ◽  
Progressive Increase ◽  
Canine Heart ◽  
Storage Mechanism ◽  
Specific Membrane ◽  
Heart Uptake ◽  
Membrane Mechanism ◽  
Stimulation Of

To study the possible role of uptake of [3H]norepinephrine ([3H]NE) as an indicator of sympathetic reinnervation of the surgically denervated canine heart, uptake was determined from multiple areas of hearts at various stages of reinnervation (1--6 mo), and these data were correlated with myocardial catecholamine content and functional response of the heart to electrical stimulation of the sympathetic nerves. Our experiments confirm that NE content correlates poorly with the degree of reinnervation of the previously denervated canine heart. There is, however, a progressive increase of [3H]NE uptake from 1 mo to 6 mo, at which time uptake has returned to approximately 57% of control values in the left atrium. The development of the storage mechanism lags far behind the specific-membrane mechanism for uptake in the reinnervating surgically denervated canine heart.

RIBOSOME-MEMBRANE INTERACTION AND PROTEIN SYNTHESIS

1973 ◽  
Vol 74 (Suppl) ◽  
pp. S192-S224 ◽  
Author(s):  
J. R. Tata
Keyword(s):  
Protein Synthesis ◽  
Growth And Development ◽  
Active Synthesis ◽  
Active Growth ◽  
Membrane Interaction ◽  
Major Function ◽  
Membrane Bound ◽  
Different Populations ◽  
Protein Secreting

ABSTRACT The role of secretion of proteins for the attachment of ribosomes to membranes has been well established. That another function must exist for membrane-ribosome interaction is suggested by observations on: (a) the active synthesis of proteins on membrane-bound ribosomes of predominantly non-protein secreting cells, and b) the massive proliferation of membrane-bound ribosomes during active growth and development of both secretory and non-secretory tissues. Literature on functional and compositional differences between membrane-bound and free ribosomes is reviewed and it is proposed that a major function of ribosome-membrane interaction is to effect a topological segregation of different populations of ribosomes synthesizing different classes of proteins.

scholarly journals Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-d-glucosamine:alpha-3-d-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase

2003 ◽  
Vol 372 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Wenli ZHANG ◽  
Pinjiang CAO ◽  
Shihao CHEN ◽  
Andrew M. SPENCE ◽  
Shaoxian ZHU ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Metal Ion ◽  
Suitable Model ◽  
Bivalent Metal ◽  
C Elegans ◽  
Membrane Bound ◽  
Specific Membrane ◽  
Optimal Ph ◽  
Nematode Development

We have previously reported three Caenorhabditis elegans genes (gly-12, gly-13 and gly-14) encoding UDP-N-acetyl-d-glucosamine:α-3-d-mannoside β1,2-N-acetylglucosaminyltransferase I (GnT I), an enzyme essential for hybrid and complex N-glycan synthesis. GLY-13 was shown to be the major GnT I in worms and to be the only GnT I cloned to date which can act on [Manα1,6(Manα1,3)Manα1,6](Manα1,3)Manβ1, 4GlcNAcβ1,4GlcNAc-R, but not on Manα1,6(Manα1,3)Manβ1-O-R substrates. We now report the kinetic constants, bivalent-metal-ion requirements, and optimal pH, temperature and Mn2+ concentration for this unusual enzyme. C. elegans glycoproteins are rich in oligomannose (Man6–9GlcNAc2) and ‘paucimannose’ Man3–5GlcNAc2(±Fuc) N-glycans, but contain only small amounts of complex and hybrid N-glycans. We show that the synthesis of paucimannose Man3GlcNAc2 requires the prior actions of GnT I, α3,6-mannosidase II and a membrane-bound β-N-acetylglucosaminidase similar to an enzyme previously reported in insects. The β-N-acetylglucosaminidase removes terminal N-acetyl-d-glucosamine from the GlcNAcβ1, 2Manα1,3Manβ- arm of Manα1,6(GlcNAcβ1,2Manα1,3) Manβ1,4GlcNAcβ1,4GlcNAc-R to produce paucimannose Man3GlcNAc2 N-glycan. N-acetyl-d-glucosamine removal was inhibited by two N-acetylglucosaminidase inhibitors. Terminal GlcNAc was not released from [Manα1,6(Manα1,3)Manα1,6] (GlcNAcβ1,2Manα1,3)Manβ1,4GlcNAcβ1,4GlcNAc-R nor from the GlcNAcβ1,2Manα1,6Manβ- arm. These findings indicate that GLY-13 plays an important role in the synthesis of N-glycans by C. elegans and that therefore the worm should prove to be a suitable model for the study of the role of GnT I in nematode development.

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