Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk

AbstractBacteriocins are antimicrobial peptides produced by bacteria and their production by health-promoting microbes is regarded as a desirable probiotic trait. We found that Lactobacillus gasseri LM19, a strain isolated from human milk, exhibits antagonistic activity against different enteropathogens and produces several bacteriocins, including a novel bacteriocin, gassericin M. These bacteriocins were purified from culture and synthesised to investigate their activity and potential synergy. L. gasseri LM19 was tested in a complex environment mimicking human colon conditions where it not only survived but expressed the seven bacteriocin genes and produced short chain fatty acids. Metagenomic analysis of these in vitro colon cultures showed that co-inoculation of L. gasseri LM19 with Clostridium perfringens gave profiles with more similarity to controls than to vessels inoculated with C. perfringens alone. This makes L. gasseri LM19 an interesting candidate for further study for maintaining homeostasis in the gut environment.

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CHARACTERIZATION OF SOME PROBIOTIC PROPERTIES OF LACTOBACILLUS GASSERI MA-1 FROM HUMAN MILK

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji ◽

10.18036/estubtdc.635397 ◽

2020 ◽

pp. 211-218

Author(s):

Meltem AŞAN ÖZÜSAĞLAM ◽

Ayşe GÜNYAKTI

Keyword(s):

Human Milk ◽

Probiotic Properties ◽

Lactobacillus Gasseri

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Human milk oligosaccharides: the novel modulator of intestinal microbiota

BMB Reports ◽

10.5483/bmbrep.2012.45.8.168 ◽

2012 ◽

Vol 45 (8) ◽

pp. 433-441 ◽

Cited By ~ 12

Author(s):

Kyung-Hun Jeong ◽

Vi Nguyen ◽

Jae-Han Kim

Keyword(s):

Human Milk ◽

Intestinal Microbiota ◽

The Novel ◽

Human Milk Oligosaccharides ◽

Milk Oligosaccharides

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Lactobacillus gasseri from human milk with probiotic potential and some technological properties

LWT ◽

10.1016/j.lwt.2019.04.043 ◽

2019 ◽

Vol 109 ◽

pp. 261-269 ◽

Cited By ~ 10

Author(s):

Ayse Gunyakti ◽

Meltem Asan-Ozusaglam

Keyword(s):

Human Milk ◽

Technological Properties ◽

Lactobacillus Gasseri ◽

Probiotic Potential

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A novel class of human milk oligosaccharides based on 6-galactosyllactose and containing N-acetylglucosamine branches extended by oligogalactoses

10.1101/2021.05.27.445961 ◽

2021 ◽

Author(s):

Franz-Georg Hanisch ◽

Clemens Kunz

Keyword(s):

Human Milk ◽

Infant Nutrition ◽

Maldi Mass Spectrometry ◽

The Novel ◽

Human Milk Oligosaccharides ◽

Milk Oligosaccharides ◽

New Class ◽

Terminal Galactose ◽

Branched Chains

Human milk oligosaccharides (HMOs) have attracted much attention in recent years not only as a prebiotic factor, but in particular as an essential component in infant nutrition related to their impact in innate immunity. The backbone structures of complex HMOs generally contain single or repetitive lacto-N-biose (type 1) or lactosamine (type 2) units in either linear or branched chains extending from a lactose core. While all known branched structures originate from 3,6-substitution of the lactosyl core galactose, we here describe a new class of HMOs that tentatively branch at terminal galactose of 6-galactosyllactose. Another novel feature of this class of HMOs was found in linear oligo-galactosyl chains linked to one of the N-acetylglucosamine (GlcNAc) branches. The novel structures exhibit general formulas with hexose vs. hexosamine contents of 5/2 to 8/2 and can be designated as high-galactose (HG)-HMOs. In addition, up to three fucosyl residues are linked to the octa- to dodecasaccharides, which were detected in two human milk samples from Lewis blood group defined donors. Structural analyses of methylated glycans and their alditols comprised MALDI mass spectrometry, ESI-(CID)MS and linkage analyses by GC-MS of the derived partially methylated alditol acetates. Enzymatic degradation by application of β1-3,4-specific galactosidase supported the presence of terminal galactose linked [beta]1-6 to one of the two GlcNAc branches.

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In vitro Assessment of Antioxidant and Antimicrobial Potential of Lactobacillus gasseri Strains Isolated from Human Milk and Infant Faeces

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.2.26 ◽

2020 ◽

Vol 14 (2) ◽

pp. 1305-1315

Author(s):

Sonakshi Rastogi ◽

Vineeta Mittal ◽

Aditi Singh

Keyword(s):

Human Milk ◽

Lactobacillus Gasseri ◽

Antimicrobial Potential ◽

In Vitro Assessment

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Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population

mSphere ◽

10.1128/mspheredirect.00152-19 ◽

2019 ◽

Vol 4 (2) ◽

Author(s):

Jan Majta ◽

Krzysztof Odrzywolek ◽

Bozena Milanovic ◽

Vladyslav Hubar ◽

Sonia Wrobel ◽

...

Keyword(s):

Human Milk ◽

The Novel ◽

Finnish Population ◽

Specific Composition ◽

Infant Gut Microbiome ◽

Novel Method ◽

Analysis Of Function ◽

Taxonomic Annotation ◽

Level Analysis

ABSTRACT A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries. Some pieces of research link such conditions with the composition of microbiota during infancy. In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO). A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides. Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations. Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations. In this paper, we present the results of function-level analysis. Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio. Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides. IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques. The significance of this research is demonstrated by the novel method used for the analysis of function-level metagenomes. BiomeScout—the presented technology—utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.

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