Development of a low-cost fermentation medium for ethanol production from biomass

1997 ◽  
Vol 47 (6) ◽  
pp. 625-629 ◽  
Author(s):  
K. L. Kadam ◽  
M. M. Newman
Keyword(s):  
Ethanol Production ◽  
Low Cost ◽  
Fermentation Medium

scholarly journals Design of Low-Cost Ethanol Production Medium from Syngas: An Optimization of Trace Metals for Clostridium ljungdahlii

Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6981
Author(s):  
Simge Sertkaya ◽  
Nuri Azbar ◽  
Haris Nalakath Abubackar ◽  
Tugba Keskin Gundogdu
Keyword(s):  
Trace Element ◽  
Ethanol Production ◽  
Low Cost ◽  
Product Distribution ◽  
Fermentation Medium ◽  
Economic Feasibility ◽  
Production Medium ◽  
Syngas Fermentation ◽  
Clostridium Ljungdahlii ◽  
The Cost

Syngas fermentation via the Wood-Ljungdahl (WL) pathway is a promising approach for converting gaseous pollutants (CO and CO2) into high-value commodities. Because the WL involves several enzymes with trace metal components, it requires an adequate supply of micronutrients in the fermentation medium for targeted bioprocessing such as bioethanol production. Plackett-Burman statistical analysis was performed to examine the most efficient trace elements (Ni, Mg, Ca, Mn, Co, Cu, B, W, Zn, Fe, and Mo) and their concentrations for Clostridium ljungdahlii on ethanol production. Overall, 1.5 to 2.5 fold improvement in ethanol production could be achieved with designed trace element concentrations. The effects of tungsten and copper on ethanol and biomass production were determined to be the most significant, respectively. The model developed was statistically significant and has the potential to significantly decrease the cost of trace element solutions by 18–22%. This research demonstrates the critical importance of optimizing the medium for syngas fermentation in terms of product distribution and economic feasibility.

Biodiesel derived crude glycerol and tuna condensate as an alternative low-cost fermentation medium for ethanol production by Enterobacter aerogenes

2019 ◽  
Vol 138 ◽  
pp. 111451 ◽  
Author(s):  
Juli Novianto Sunarno ◽  
Poonsuk Prasertsan ◽  
Wiriya Duangsuwan ◽  
Benjamas Cheirsilp ◽  
Kanokphorn Sangkharak
Keyword(s):  
Ethanol Production ◽  
Crude Glycerol ◽  
Low Cost ◽  
Enterobacter Aerogenes ◽  
Fermentation Medium

Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium

2012 ◽  
Vol 160 (3-4) ◽  
pp. 229-235 ◽  
Author(s):  
Yu Shen ◽  
Jin-Song Guo ◽  
You-Peng Chen ◽  
Hai-Dong Zhang ◽  
Xu-Xu Zheng ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Nitrogen Source ◽  
Sweet Potato ◽  
Low Cost ◽  
Fuel Ethanol ◽  
High Gravity

UJI KINERJA PADA PROSES PRODUKSI ETANOL MELALUI KOMBINASI PROSES FERMENTASI DAN STRIPPING

EKUILIBIUM ◽  
2012 ◽  
Vol 11 (2) ◽  
Author(s):  
Margono Margono
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Renewable Energy ◽  
Production Process ◽  
Ethanol Production ◽  
Anaerobic Fermentation ◽  
Fermentation Medium ◽  
High Price ◽  
Trickle Bed ◽  
Trickle Bed Bioreactor ◽  
Biofilm Development

<p><strong><em>Abstract:</em></strong> <em>Renewable energy necesity have promote research on ethanol production technology. Ethanol is the potential renewable energy substituting gasoline. However, the conventional problem is high price of the ethanol. The objective of this research was to test the performance of alternative process in producing ethanol, i.e. combination of fermentation process with ethanol stripping in trickle bed bioreactor. The experimental was using Saccharomyces cerevisiae FNCC 3012 and sugarcane bagass as bed particle. It was devided into 2 process steps of biofilm development and ethanol production. Biofilm development was done by circulating medium in bioreactor aerobically. Duration of the biofilm development was 24 hours and followed by ethanol production step which was combinating anaerobic fermentation and stripping process using nitrogen. Production process was conducted for 36 hours lifetime. This method resulted biofilm developing in fermentation medium, not on baggas surfaces. Consequently, ethanol production happened in circulated fermentation medium. The productivity of this method of ethanol production process was not better than the conventional process. Neverherless, the experimental showed that the product stripping and fermentation could be done simultaneously. The stripping process increased ethanol product concentration up to 25% higher than in the broth</em>.</p><p> <strong><em>Keywords:</em></strong> <em>ethanol, Saccharomyces cerevisiae FNCC 3012, trickle bed bioreactor, stripping, biofilm</em></p>

scholarly journals Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

2020 ◽  
Author(s):  
Yazhou Li ◽  
Jianghan Wang ◽  
Luxin Ke ◽  
Xiuyun Zhao ◽  
Gaofu Qi
Keyword(s):  
Fulvic Acid ◽  
Low Cost ◽  
Value Added ◽  
Fermentation Medium ◽  
Systemic Resistance ◽  
Callose Deposition ◽  
Wastewater Disposal ◽  
Industry Waste ◽  
A Value ◽  
Ethylene Signalling

Abstract Background: Molasses is a wildly used feedstock for fermentation, but it poses a severe wastewater-disposal problem worldwide. Recently, the wastewater produced by yeast during molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied in soil. In this study, the low-cost FA powder was bioconverted into a value-added product, γ-PGA, by a glutamate independent producer, Bacillus velezensis GJ11. Results: With FA powder, the substrates of sodium glutamate and citrate sodium used in medium were decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium. In the optimized FA powder fermentation medium, the γ-PGA was produced with its maximum concentration at 42.55 g/L and a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolyzates was dependent on the ethylene signalling and NPR1. Conclusions: To our knowledge, this is the first report of using the industry waste, FA powder, as a sustainable substrate for the microbial synthesis of γ-PGA. This bioprocess can not only develop a new way of FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

scholarly journals Effect of initial fermentation medium on bioacetone, biobutanol, and bioethanol (BioABE) production from fermentable sugars of Acacia mangium using Clostridium acetobutylicum YM1

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6912-6927
Author(s):  
Rafidah Jalil ◽  
Mohd Sahaid Kalil ◽  
Norliza Abd Rahman ◽  
Abdulalati Ibrahim Al-Tabib ◽  
Aidil Abdul Hamid ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Low Cost ◽  
White Rot Fungi ◽  
Acacia Mangium ◽  
Product Yield ◽  
Fermentation Medium ◽  
White Rot ◽  
Fermentable Sugars ◽  
Process Variables ◽  
Initial Ph

Bioacetone, biobutanol, and bioethanol (BioABE) production is dependent on the fermentable sugars produced from lignocellulosic biomass and on the composition and initial pH of the medium. Understanding these process variables and their interconnectedness could enhance the BioABE product yield. Acacia mangium is available abundantly and it is a potential feedstock for BioABE production. In this study, BioABE was produced from fermentable sugars of A. mangium using Clostridium acetobutylicum YM1. Alkaline treated A. mangium (70 °C, 3 h, 5.50 %w/v NaOH) was further hydrolyzed via enzymatic hydrolysis using a multi-enzyme of white rot fungi to convert it into fermentable sugars. Approximately 15 g/L of fermentable sugars was produced from A. mangium (100 g/L) and was used for BioABE production in comparison with glucose. Initial findings showed that only 0.94 g/L of BioABE was produced in comparison with glucose (2.86 g/L) at a pH of 6.2. Decreasing the initial pH of the medium to 4.50 increased the BioABE (2.87 g/L), and after the medium was supplemented with tryptone-yeast-acetate (TYA), the BioABE yield increased by more than 100% to 6.84 g/L. This study discovered that BioABE produced from A. mangium was comparable to using commercial glucose, thus offering high potential as a low-cost feedstock.

scholarly journals Evaluation of sugar yeast consumption by measuring electrical medium resistance

2019 ◽  
Vol 4 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Martín L. Zamora ◽  
Gabriel A. Ruiz ◽  
Carmelo J. Felice
Keyword(s):  
Low Cost ◽  
Fermentation Medium ◽  
New Method ◽  
Stainless Steel Electrode ◽  
Yeast Suspension ◽  
Sugar Consumption ◽  
Counter Electrodes ◽  
Steel Electrode ◽  
Medium Resistance ◽  
Novel Method

Abstract The real-time monitoring of alcoholic fermentation (sugar consumption) is very important in industrial processes. Several techniques (i.e., using a biosensor) have been proposed to realize this goal. In this work, we propose a new method to follow sugar yeast consumption. This novel method is based on the changes in the medium resistance (Rm) that are induced by the CO2 bubbles produced during a fermentative process. We applied a 50-mV and 700-Hz signal to 75 ml of a yeast suspension in a tripolar cell. A gold electrode was used as the working electrode, whereas an Ag/AgCl electrode and a stainless-steel electrode served as the reference and counter electrodes, respectively. We then added glucose to the yeast suspension and obtained a 700% increase in the Rm after 8 minutes. The addition of sucrose instead of glucose as the carbon source resulted in a 1200% increase in the Rm. To confirm that these changes are the result of CO2 bubbles in the fermentation medium, we designed a tetrapolar cell in which CO2 gas was insufflated at the bottom of the cell and concluded that the changes were due to CO2 bubbles produced during the fermentation. Consequently, this new method is a low-cost and rapid technology to follow the sugar consumption in yeast.

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