Study of Superoxide Dismutase Activity in Long-Term Cultivated Artemisia and Althaea “hairy” Roots

Background: Cardiac hypertrophy involves marked wall thickening or chamber enlargement. If sustained, this condition will lead to dysfunctional mitochondria and oxidative stress. Mitochondria have ATP-sensitive K+ channels (mitoKATP) in the inner membrane that modulate the redox status of the cell. Objective: We investigated the in vivo effects of mitoKATP opening on oxidative stress in isoproterenol- induced cardiac hypertrophy. Methods: Cardiac hypertrophy was induced in Swiss mice treated intraperitoneally with isoproterenol (ISO - 30 mg/kg/day) for 8 days. From day 4, diazoxide (DZX - 5 mg/kg/day) was used in order to open mitoKATP (a clinically relevant therapy scheme) and 5-hydroxydecanoate (5HD - 5 mg/kg/day) or glibenclamide (GLI - 3 mg/kg/day) were used as mitoKATP blockers. Results: Isoproterenol-treated mice had elevated heart weight/tibia length ratios (HW/TL). Additionally, hypertrophic hearts had elevated levels of carbonylated proteins and Thiobarbituric Acid Reactive Substances (TBARS), markers of protein and lipid oxidation. In contrast, mitoKATP opening with DZX avoided ISO effects on gross hypertrophic markers (HW/TL), carbonylated proteins and TBARS, in a manner reversed by 5HD and GLI. Moreover, DZX improved mitochondrial superoxide dismutase activity. This effect was also blocked by 5HD and GLI. Additionally, ex vivo treatment of isoproterenol- induced hypertrophic cardiac tissue with DZX decreased H2O2 production in a manner sensitive to 5HD, indicating that this drug also acutely avoids oxidative stress. Conclusion: Our results suggest that diazoxide blocks oxidative stress and reverses cardiac hypertrophy. This pharmacological intervention could be a potential therapeutic strategy to prevent oxidative stress associated with cardiac hypertrophy.

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Location and effects of long-term NaCl stress on superoxide dismutase and ascorbate peroxidase isoenzymes of pea (Pisum sativum cv. Puget) chloroplasts

Journal of Experimental Botany ◽

10.1093/jxb/erh013 ◽

2003 ◽

Vol 55 (394) ◽

pp. 119-130 ◽

Cited By ~ 125

Author(s):

J. M. Gomez

Keyword(s):

Superoxide Dismutase ◽

Pisum Sativum ◽

Ascorbate Peroxidase ◽

Nacl Stress ◽

Peroxidase Isoenzymes

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Complexation of Mn(II) by Rigid Pyclen Diacetates: Equilibrium, Kinetic, Relaxometric, Density Functional Theory, and Superoxide Dismutase Activity Studies

Inorganic Chemistry ◽

10.1021/acs.inorgchem.0c03276 ◽

2020 ◽

Author(s):

Zoltán Garda ◽

Enikő Molnár ◽

Nadège Hamon ◽

José Luis Barriada ◽

David Esteban-Gómez ◽

...

Keyword(s):

Density Functional Theory ◽

Superoxide Dismutase ◽

Density Functional ◽

Superoxide Dismutase Activity ◽

Functional Theory

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Two New Compounds From the Heartwood of Dalbergia melanoxylon and Their Protective Effect on Hypoxia/Reoxygenation Injury in H9c2

Natural Product Communications ◽

10.1177/1934578x20987776 ◽

2021 ◽

Vol 16 (1) ◽

pp. 1934578X2098777

Author(s):

Yang Liu ◽

Ni Zhang ◽

Jun-wei He ◽

Lan-ying Chen ◽

Li Yang ◽

...

Keyword(s):

Superoxide Dismutase ◽

Lactate Dehydrogenase ◽

Protective Effect ◽

Superoxide Dismutase Activity ◽

Spectral Evidence ◽

Reoxygenation Injury ◽

Dalbergia Melanoxylon ◽

New Compounds ◽

Hypoxia Reoxygenation

A new neoflavonoid, named as (7 R)-(-)-3′,5-dihydroxy-4′,2,4-trimethoxy-dalbergiquinol (1) and a new phenanthrenedione, named as 3′,7-dihydroxy-3,6- dimethoxy-9-phenyl-1,4-phenanthrenedione (2), together with 4 known compounds, 5- O-methyldalbergiphenol (3), 3′,7-dihydroxy-4′,3,6-trimethoxy-9-phenyl-1,4-phenanthrenedione (4), (+)-obtusafuran (5), and melanoxin (6) were isolated from the heartwood of Dalbergia melanoxylon. Their structures were elucidated on the basis of chemical and spectral evidence, as well as by comparison with literature data. Moreover, compound 1 showed a protective effect on hypoxia/reoxygenation injury in H9c2 at 10.0 μM by decreasing lactate dehydrogenase and malondialdehyde activity and enhancing superoxide dismutase activity.

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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Zygote ◽

10.1017/s0967199419000467 ◽

2019 ◽

Vol 27 (6) ◽

pp. 432-435

Author(s):

Thais Rose dos Santos Hamilton ◽

Gabriela Esteves Duarte ◽

José Antonio Visintin ◽

Mayra Elena Ortiz D’Ávila Assumpção

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Heat Stress ◽

Glutathione Peroxidase ◽

Cell Types ◽

Treated Group ◽

Oxidative Balance ◽

Testicular Cells

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

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