Grr1p is required for transcriptional induction of amino acid permease genes and proper transcriptional regulation of genes in carbon metabolism of Saccharomyces cerevisiae

2004 ◽  
Vol 47 (3) ◽  
pp. 139-149 ◽  
Author(s):  
Nadine Eckert-Boulet ◽  
Birgitte Regenberg ◽  
Jens Nielsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Regulation ◽  
Amino Acid ◽  
Carbon Metabolism ◽  
Amino Acid Permease ◽  
Transcriptional Induction

scholarly journals Amino Acid Signaling in Saccharomyces cerevisiae: a Permease-Like Sensor of External Amino Acids and F-Box Protein Grr1p Are Required for Transcriptional Induction of the AGP1 Gene, Which Encodes a Broad-Specificity Amino Acid Permease

1999 ◽  
Vol 19 (2) ◽  
pp. 989-1001 ◽  
Author(s):  
Ismaïl Iraqui ◽  
Stephan Vissers ◽  
Florent Bernard ◽  
Johan-Owen de Craene ◽  
Eckhard Boles ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Amino Acid ◽  
Glucose Sensors ◽  
Amino Acid Permease ◽  
Transporter Family ◽  
F Box Protein ◽  
Transcriptional Induction ◽  
Amino Acid Signaling ◽  
Broad Specificity

ABSTRACT The SSY1 gene of Saccharomyces cerevisiaeencodes a member of a large family of amino acid permeases. Compared to the 17 other proteins of this family, however, Ssy1p displays unusual structural features reminiscent of those distinguishing the Snf3p and Rgt2p glucose sensors from the other proteins of the sugar transporter family. We show here that SSY1 is required for transcriptional induction, in response to multiple amino acids, of theAGP1 gene encoding a low-affinity, broad-specificity amino acid permease. Total noninduction of the AGP1 gene in thessy1Δ mutant is not due to impaired incorporation of inducing amino acids. Conversely, AGP1 is strongly induced by tryptophan in a mutant strain largely deficient in tryptophan uptake, but it remains unexpressed in a mutant that accumulates high levels of tryptophan endogenously. Induction of AGP1requires Uga35p(Dal81p/DurLp), a transcription factor of the Cys6-Zn2 family previously shown to participate in several nitrogen induction pathways. Induction of AGP1by amino acids also requires Grr1p, the F-box protein of the SCFGrr1 ubiquitin-protein ligase complex also required for transduction of the glucose signal generated by the Snf3p and Rgt2p glucose sensors. Systematic analysis of amino acid permease genes showed that Ssy1p is involved in transcriptional induction of at least five genes in addition to AGP1. Our results show that the amino acid permease homologue Ssy1p is a sensor of external amino acids, coupling availability of amino acids to transcriptional events. The essential role of Grr1p in this amino acid signaling pathway lends further support to the hypothesis that this protein participates in integrating nutrient availability with the cell cycle.

Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2

2001 ◽  
Vol 264 (5) ◽  
pp. 613-622 ◽  
Author(s):  
P. Nielsen ◽  
B. van den Hazel ◽  
T. Didion ◽  
M. de Boer ◽  
M. Jørgensen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Regulation ◽  
Amino Acid ◽  
Amino Acid Permease

scholarly journals Transcriptional Induction by Aromatic Amino Acids in Saccharomyces cerevisiae

1999 ◽  
Vol 19 (5) ◽  
pp. 3360-3371 ◽  
Author(s):  
Ismaïl Iraqui ◽  
Stéphan Vissers ◽  
Bruno André ◽  
Antonio Urrestarazu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Aromatic Amino Acids ◽  
Positive Control ◽  
General Factor ◽  
Amino Acid Transporters ◽  
Amino Acid Permease ◽  
Gene Encoding ◽  
Inducer Exclusion ◽  
Transcriptional Induction

ABSTRACT Aromatic aminotransferase II, product of the ARO9 gene, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism in Saccharomyces cerevisiae. ARO9 expression is under the dual control of specific induction and nitrogen source regulation. We have here identified UASaro, a 36-bp upstream element necessary and sufficient to promote transcriptional induction of reporter gene expression in response to tryptophan, phenylalanine, or tyrosine. We then isolated mutants in which UASaro-mediated ARO9 transcription is partially or totally impaired. Mutations abolishingARO9 induction affect a gene called ARO80(YDR421w), coding for a Zn2Cys6 family transcription factor. A sequence highly similar to UASaro was found upstream from theYDR380w gene encoding a homolog of bacterial indolepyruvate decarboxylase. In yeast, this enzyme is postulated to catalyze the second step of tryptophan catabolism to tryptophol. We show that ARO9 and YDR380w(named ARO10) have similar patterns of transcriptional regulation and are both under the positive control of Aro80p. Nitrogen regulation of ARO9 expression seems not directly to involve the general factor Ure2p, Gln3p, Nil1p, Uga43p, or Gzf3p.ARO9 expression appears, rather, to be mainly regulated by inducer exclusion. Finally, we show that Gap1p, the general amino acid permease, and Wap1p (Ycl025p), a newly discovered inducible amino acid permease with broad specificity, are the main aromatic amino acid transporters for catabolic purposes.

Ammonia regulation of amino acid permeases in Saccharomyces cerevisiae

1983 ◽  
Vol 3 (4) ◽  
pp. 672-683
Author(s):  
W E Courchesne ◽  
B Magasanik
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Cytoplasmic Membrane ◽  
Nitrogen Sources ◽  
Amino Acid Permease ◽  
Inducer Exclusion ◽  
Amino Acid Permeases ◽  
General Amino Acid Permease ◽  
State Growth ◽  
And Control

The activities of the proline-specific permease (PUT4) and the general amino acid permease (GAP1) of Saccharomyces cerevisiae vary 70- to 140-fold in response to the nitrogen source of the growth medium. The PUT4 and GAP1 permease activities are regulated by control of synthesis and control of activity. These permeases are irreversibly inactivated by addition of ammonia or glutamine, lowering the activity to that found during steady-state growth on these nitrogen sources. Mutants altered in the regulation of the PUT4 permease (Per-) have been isolated. The mutations in these strains are pleiotropic and affect many other permeases, but have no direct effect on various cytoplasmic enzymes involved in nitrogen assimilation. In strains having one class of mutations (per1), ammonia inactivation of the PUT4 and GAP1 permeases did not occur, whereas glutamate and glutamine inactivation did. Thus, there appear to be two independent inactivation systems, one responding to ammonia and one responding to glutamate (or a metabolite of glutamate). The mutations were found to be nuclear and recessive. The inactivation systems are constitutive and do not require transport of the effector molecules per se, apparently operating on the inside of the cytoplasmic membrane. The ammonia inactivation was found not to require a functional glutamate dehydrogenase (NADP). These mutants were used to show that ammonia exerts control of arginase synthesis largely by inducer exclusion. This may be the primary mode of nitrogen regulation for most nitrogen-regulated enzymes of S. cerevisiae.

scholarly journals Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake.

1994 ◽  
Vol 14 (10) ◽  
pp. 6597-6606 ◽  
Author(s):  
A Schmidt ◽  
M N Hall ◽  
A Koller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
T Cell Activation ◽  
Cell Activation ◽  
Amino Acid Permease ◽  
High Affinity ◽  
New Genes ◽  
Eukaryotic Microorganisms ◽  
Amino Acid Permeases ◽  
Tryptophan Uptake

The macrocyclic lactone FK506 exerts immunosuppressive effects on T lymphocytes by interfering with signal transduction leading to T-cell activation and also inhibits the growth of eukaryotic microorganisms, including Saccharomyces cerevisiae. We reported previously that an FK506-sensitive target in S. cerevisiae is required for amino acid import and that overexpression of two new genes, TAT1 and TAT2 (formerly called TAP1 and TAP2), confers resistance to the drug. Here we report that TAT1 and TAT2 encode novel members of the yeast amino acid permease family composed of integral membrane proteins that share 30 to 40% identity. TAT1 is the tyrosine high-affinity transporter, which also mediates low-affinity or low-capacity uptake of tryptophan. TAT2 is the tryptophan high-affinity transporter. FK506 does not reduce the levels of TAT1 and TAT2 transcripts, indicating that the inhibition of amino acid transport by the drug is posttranscriptional.

scholarly journals The Saccharomyces cerevisiae YCC5(YCL025c) Gene Encodes an Amino Acid Permease, Agp1, Which Transports Asparagine and Glutamine

1998 ◽  
Vol 180 (9) ◽  
pp. 2556-2559 ◽  
Author(s):  
James L. Schreve ◽  
Jennifer K. Sin ◽  
Jinnie M. Garrett
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Kinetic Analysis ◽  
Amino Acid Permease ◽  
High Affinity ◽  
Nitrogen Repression

ABSTRACT The yeast YCC5 gene encodes a putative amino acid permease and is homologous to GNP1 (encoding a high-affinity glutamine permease). Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression.

scholarly journals Different Ubiquitin Signals Act at the Golgi and Plasma Membrane to Direct GAP1 Trafficking

2008 ◽  
Vol 19 (7) ◽  
pp. 2962-2972 ◽  
Author(s):  
April L. Risinger ◽  
Chris A. Kaiser
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Plasma Membrane ◽  
Amino Acid ◽  
Protein Sorting ◽  
High Capacity ◽  
Amino Acid Permease ◽  
Acid Addition ◽  
Amino Acid Levels ◽  
General Amino Acid Permease

The high capacity general amino acid permease, Gap1p, in Saccharomyces cerevisiae is distributed between the plasma membrane and internal compartments according to availability of amino acids. When internal amino acid levels are low, Gap1p is localized to the plasma membrane where it imports available amino acids from the medium. When sufficient amino acids are imported, Gap1p at the plasma membrane is endocytosed and newly synthesized Gap1p is delivered to the vacuole; both sorting steps require Gap1p ubiquitination. Although it has been suggested that identical trans-acting factors and Gap1p ubiquitin acceptor sites are involved in both processes, we define unique requirements for each of the ubiquitin-mediated sorting steps involved in delivery of Gap1p to the vacuole upon amino acid addition. Our finding that distinct ubiquitin-mediated sorting steps employ unique trans-acting factors, ubiquitination sites on Gap1p, and types of ubiquitination demonstrates a previously unrecognized level of specificity in ubiquitin-mediated protein sorting.

scholarly journals Constitutive and Hyperresponsive Signaling by Mutant Forms of Saccharomyces cerevisiae Amino Acid Sensor Ssy1

2003 ◽  
Vol 2 (5) ◽  
pp. 922-929 ◽  
Author(s):  
Richard F. Gaber ◽  
Kim Ottow ◽  
Helge A. Andersen ◽  
Morten C. Kielland-Brandt
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Direct Interaction ◽  
Acid Transport ◽  
Amino Acid Permease ◽  
Amino Acid Permeases ◽  
Mutant Forms ◽  
Amino Acid Sensor ◽  
Transcriptional Induction ◽  
Amino Acid Signaling

ABSTRACT Sensing of extracellular amino acids results in transcriptional induction of amino acid permease genes in yeast. Ssy1, a membrane protein resembling amino acid permeases, is required for signaling but is apparently unable to transport amino acids and is thus believed to be a sensor. By using a novel genetic screen in which potassium uptake was made dependent on amino acid signaling, we obtained gain-of-function mutations in SSY1. Some alleles confer inducer-independent signaling; others increase the apparent affinity for inducers. The results reveal that amino acid transport is not required for signaling and support the notion that sensing by Ssy1 occurs via its direct interaction with extracellular amino acids.

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