GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata

2004 ◽  
Vol 23 (6) ◽  
pp. 391-396 ◽  
Author(s):  
C. H. Fu ◽  
C. L. Chen ◽  
W. W. Guo ◽  
X. X. Deng
Keyword(s):  
Somatic Hybrid ◽  
Rflp Analysis ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Pcr Rflp ◽  
Intergeneric Somatic Hybrid

scholarly journals Somatic Hybridization of Complementary Citrus Rootstock:Five New Hybrids

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 812-813 ◽  
Author(s):  
Jude W. Grosser ◽  
Frederick G. Gmitter ◽  
J.L. Chandler ◽  
Eliezer S. Louzada
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybridization ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Trifoliate Orange ◽  
Hybrid Plants ◽  
Regenerated Plants ◽  
Citrus Rootstock ◽  
Sexual Hybrid ◽  
Cleopatra Mandarin

Protoplasm culture following polyethylene glycol-induced fusion resulted in the regeneration of tetraploid somatic hybrid plants from the following attempted parental combinations: Cleopatra mandarin (Citrus reticulata Blanco) + Argentine trifoliate orange [Poncirus trifoliata (L.) Raf.]; `Succari' sweet orange [C. sinensis (L.) Osb.] + Argentine trifoliate orange; sour orange (C. aurantium L.) + Flying Dragon trifoliate orange (P. trifolita); sour orange + Rangpur (C. limonia Osb.); and Milam lemon (purported sexual hybrid of C. jambhiri Lush × C. sinensis) + Sun Chu Sha mandarin (C. reticulate Blanco). Protoplasm isolation, fusion, and culture were conducted according to previously published methods. Regenerated plants were classified according to leaf morphology, chromosome number, and peroxidase, phosphoglucomutase, and phosphoglucose isomerase leaf isozyme profiles. All of the somatic hybrid plants were tetraploid, as expected (2n = 4x = 36), and all five selections have been propagated and entered into commercial citrus rootstock trials.

scholarly journals Eight New Somatic Hybrid Citrus Rootstocks with Potential for Improved Disease Resistance

HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 1033-1036 ◽  
Author(s):  
Eliezer S. Louzada ◽  
Jude W. Grosseti ◽  
Frederick G. Gmitter ◽  
Beatriz Nielsen ◽  
J.L. Chandler ◽  
...  
Keyword(s):  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Sweet Orange ◽  
Field Trials ◽  
Poncirus Trifoliata ◽  
Sour Orange ◽  
Rough Lemon ◽  
Hybrid Plants ◽  
Volkamer Lemon ◽  
Cleopatra Mandarin

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of vigorous tetraploid somatic hybrid plants from eight complementary parental rootstock combinations: Citrus reticulata Blanco (Cleopatra mandarin) + C. aurantium L. (sour orange), C. reticulata (Cleopatra mandarin) + C. jambhiri Lush (rough lemon), C. reticulata (Cleopatra mandarin) + C. volkameriana Ten. & Pasq. (Volkamer lemon), C. reticulata (Cleopatra mandarin) + C. limonia Osb. (Rang-pur), C. sinensis (L.) Osb. (Hamlin sweet orange) + C. limonia (Rangpur), C. aurantium (sour orange) + C. volkameriana (Volkamer lemon) zygotic seedling, C. auruntium hybrid (Smooth Flat Seville) + C. jambhiri (rough lemon), and C. sinensis (Valencia sweet orange) + Carrizo citrange [C. paradisi Macf. × Poncirus trifoliata (L.) Raf.]. Diploid plants were regenerated from nonfused callus-derived protoplasts of Valencia sweet orange and Smooth Flat Seville and from nonfused leaf protoplasts of sour orange, Rangpur, rough lemon, and Volkamer lemon. Regenerated plants were classified according to leaf morphology, chromosome number, and leaf isozyme profiles. All somatic hybrid plants were tetraploid (2n = 4× = 36). One autotetraploid plant of the Volkamer lemon zygotic was recovered, apparently resulting from a homokaryotic fusion. These eight new citrus somatic hybrids have been propagated and entered into field trials.

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp

Genetic and Biochemical Markers of Ovarian Function and Their Impact on Women Reproductive Status

2020 ◽  
Vol 71 (10) ◽  
pp. 212-217
Author(s):  
Adina-Elena Tanase ◽  
Roxana Popescu ◽  
Mircea Onofriescu ◽  
Roxana Daniela Matasariu
Keyword(s):  
Ovarian Function ◽  
Rflp Analysis ◽  
Fsh Receptor ◽  
Gene Encoding ◽  
Female Population ◽  
Fshr Gene ◽  
Pcr Rflp ◽  
Regulation Mechanisms ◽  
American Society ◽  
Dependent Mechanism

Endometriosis is a disease very common nowadays affecting 1-2% of the female population, by estrogen-dependent mechanism. The identification of mutations in the gene encoding for the FSH receptor (FSHR) has been reported since 1995. Physiology teaches us that follicle-stimulating hormone (FSH) is a hormone that is vital in the steroidogenesis regulation mechanisms, while FSH receptor (FSHR) activation helps to promote folliculogenesis and estrogensynthesis. Therefore, studies to show if there are any correlations between endometriosis and FSHR are acquired. Genotyping of FSHR gene polymorphisms were performed using PCR - Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. We analysed a total of 78 patients, 44 infertile patients with endometriosis and 34 controls (non-infertile, pregnant patients). The endometriosis group included women with diagnosis of endo-metriosis confirmed by laparoscopy and /or laparotomy and histological evidence of disease with the endometriosis staging according to American Society for Reproductive Medicine (ASRM). Corroborated with the severity of endometriosis, A919G and A2039G tests found that 71.4% of the M (GG) results were associated with primary infertility, not statistically significant (p=0.994) and 42.9% of the total M results had moderate or severe forms of endometriosis (p = 0.185). The genetic involvement in different pathologies such as endometriosis, has yet to be understood, but knowing more about its mechanism, will help physician target the disease at a more profound level.

RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus

2005 ◽  
Vol 71 (3) ◽  
pp. 243-246 ◽  
Author(s):  
Eiko Nakazono-Nagaoka ◽  
Masako Suzuki ◽  
Yoshitaka Kosaka ◽  
Tomohide Natsuaki
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rflp Analysis ◽  
Cross Protection ◽  
Rt Pcr ◽  
Pcr Rflp ◽  
Attenuated Isolate
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