Expression of adhesion molecules and monocyte chemoattractant protein-1 (MCP-1) in the spinal cord lesions in HTLV-I-associated myelopathy

1996 ◽  
Vol 91 (4) ◽  
pp. 343-350 ◽  
Author(s):  
F. Umehara ◽  
Shuji Izumo ◽  
Motohiro Takeya ◽  
Eiichi Sato ◽  
Mitsuhiro Osame ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Adhesion Molecules ◽  
Spinal Cord Lesions ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein

scholarly journals Altered Expression of Heat Shock Protein-27 and Monocyte Chemoattractant Protein-1 after Acute Spinal Cord Injury: A Pilot Study

2019 ◽  
Vol 10 (03) ◽  
pp. 452-458
Author(s):  
Vidyasagar Boraiah ◽  
Shweta Modgil ◽  
Kaushal Sharma ◽  
Vivek Podder ◽  
Madhava Sai Sivapuram ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Caspase 3 ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Hsp 27 ◽  
Cord Injury

Abstract Background Spinal cord injury (SCI) leads to serious complications involving primary trauma and progressive loss due to inflammation, local ischemia, or infection. Despite a worldwide annual incidence of 15 to 40 cases per million, methylprednisolone is the only treatment available to alleviate neurologic dysfunction; therefore, research is currently focused on identifying novel targets by biochemical and molecular studies. Purpose Here, we investigated the expression of various molecular markers at the messenger ribonucleic acid (mRNA) and protein level at day 0 and day 30 post-SCI. Methods Enzyme-linked immunosorbent assay (ELISA) was performed to determine the expression of CASPASE-3 and heat shock protein-27 (HSP-27) in serum samples. Real-time polymerase chain reaction (RT-PCR) was performed to determine the level of mRNA expression of vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, HSP-27, monocyte chemoattractant protein-1 (MCP-1), and CASPASE-3. Results HSP-27 expression at day 30, as compared with day 0, showed significant downregulation. In contrast, there was elevated expression of MCP-1. ELISA analysis showed no significant change in the expression of CASPASE-3 or HSP-27. Conclusion There may be possible opposing role of HSP-27 and MCP-1 governing SCI. Their association can be studied by designing in vitro studies.

Increased levels of adhesion molecules and monocyte chemoattractant protein-1 during persistent atrial fibrillation

Heart Rhythm ◽  
2005 ◽  
Vol 2 (5) ◽  
pp. S21
Author(s):  
Matthias Hammwoehner ◽  
Jutta Dierkes ◽  
Annelore Ittenson ◽  
Alicja Bukowska ◽  
Uwe Lendeckel ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Adhesion Molecules ◽  
Persistent Atrial Fibrillation ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein

scholarly journals Fluvastatin increases the expression of adhesion molecules, monocyte chemoattractant protein-1 and tissue factor in HUVEC stimulated by patient IgG fractions containing antiphospholipid antibodies

2005 ◽  
Vol 93 (02) ◽  
pp. 339-345 ◽  
Author(s):  
Sylvie Dunoyer-Geindre ◽  
Yordanka Dimitrova ◽  
Richard Fish ◽  
Nathalie Satta ◽  
Guido Reber ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Adhesion Molecules ◽  
Antiphospholipid Antibodies ◽  
Leukocyte Adhesion ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Qrt Pcr ◽  
Monocyte Chemoattractant Protein ◽  
Increased Risk ◽  
Leukocyte Adhesion Molecules

SummaryThe presence of antiphospholipid antibodies (APLA) is associated with an increased risk of recurrent thrombosis and pregnancy loss. APLA are able to activate endothelial cells (EC) and induce an increase in the expression of inflammatory marker proteins, such as leukocyte adhesion molecules, tissue factor or the monocyte chemoattractant protein-1 (MCP-1). Our objective was to investigate the effect of statins on EC activation induced by APLA in vitro. IgG was purified from the plasma of six patients with APLA and from healthy controls. EC were incubated with patient IgG or with control IgG, in the presence or absence of 5μM of fluvastatin, and expression of the leukocyte adhesion molecules, VCAM-1 and E-selectin, analyzed by flow cytometry and by quantitative reverse transcriptase-PCR (QRT-PCR).The expression of tissue factor and the chemokine MCP-1 was analyzed by QRT-PCR alone. Incubation of EC with patient IgG increased the expression of VCAM-1, E-selectin, tissue factor and MCP-1. Prior treatment of the cells with fluvastatin further increased the expression of these proteins. The fluvastatin effect was reversed by co-incubation with mevalonate or geranylgeranylpyrophosphate and mimicked by the geranylgeranyl transferase inhibitor GGTI-286. Our results show that in cultured human EC, statins increase the extent of inflammatory activation induced by APLA. This effect appears to be mediated by an inhibitory effect of statins on one or more geranylgeranylated protein(s).

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