Apoptotic biomarkers in cumulus cells in relation to embryo quality in polycystic ovary syndrome

2017 ◽  
Vol 296 (6) ◽  
pp. 1219-1227 ◽  
Author(s):  
Ensieh Salehi ◽  
Reza Aflatoonian ◽  
Ashraf Moeini ◽  
Nazila Yamini ◽  
Ebrahim Asadi ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Ovary Syndrome

Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome

2021 ◽  
Author(s):  
Mojtaba Masoudi ◽  
Nazila Yamini ◽  
Fahimeh Salehi ◽  
Reza Aflatoonian ◽  
Maryam Azizi Kutenaee ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Notch Signaling Pathway ◽  
Ovary Syndrome

The role of vitamin D in metabolic and reproductive disturbances of polycystic ovary syndrome: A narrative mini-review

2020 ◽  
pp. 1-8
Author(s):  
Daniela Menichini ◽  
Gianpiero Forte ◽  
Beatrice Orrù ◽  
Giuseppe Gullo ◽  
Vittorio Unfer ◽  
...  
Keyword(s):  
Vitamin D ◽  
Polycystic Ovary Syndrome ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Vitamin D Supplementation ◽  
Endometrial Receptivity ◽  
Metabolic Disturbances ◽  
Ovary Syndrome ◽  
Beneficial Role

Abstract. Vitamin D is a secosteroid hormone that plays a pivotal role in several metabolic and reproductive pathways in humans. Increasing evidence supports the role of vitamin D deficiency in metabolic disturbances and infertility in women with polycystic ovary syndrome (PCOS). Indeed, supplementation with vitamin D seems to have a beneficial role on insulin resistance and endometrial receptivity. On the other hand, exceedingly high levels of vitamin D appear to play a detrimental role on oocytes development and embryo quality. In the current review, we summarize the available evidence about the topic, aiming to suggest the best supplementation strategy in women with PCOS or, more generally, in those with metabolic disturbances and infertility. Based on the retrieved data, vitamin D seems to have a beneficial role on IR, insulin sensitivity and endometrial receptivity, but high levels and incorrect timing of administration seem to have a detrimental role on oocytes development and embryo quality. Therefore, we encourage a low dose supplementation (400–800 IU/day) particularly in vitamin D deficient women that present metabolic disturbances like PCOS. As far as the reproductive health, we advise vitamin D supplementation in selected populations, only during specific moments of the ovarian cycle, to support the luteal phase. However, ambiguities about dosage and timing of the supplementation still emerge from the clinical studies published to date and further studies are required.

Novel circular RNA expression in the cumulus cells of patients with polycystic ovary syndrome

2019 ◽  
Vol 299 (6) ◽  
pp. 1715-1725 ◽  
Author(s):  
Zhi Ma ◽  
Huishan Zhao ◽  
Yan Zhang ◽  
Xiaoyan Liu ◽  
Cuifang Hao
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Circular Rna ◽  
Rna Expression ◽  
Ovary Syndrome

scholarly journals Abnormal expression of TLRs may play a role in lower embryo quality of women with polycystic ovary syndrome

2016 ◽  
Vol 62 (5) ◽  
pp. 353-358 ◽  
Author(s):  
Bao-Xia Gu ◽  
Xue Wang ◽  
Bao-Li Yin ◽  
Hai-Bin Guo ◽  
He-Long Zhang ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Abnormal Expression

N-Acetylcysteine improves oocyte and embryo quality in polycystic ovary syndrome patients undergoing intracytoplasmic sperm injection: an alternative to metformin

2016 ◽  
Vol 28 (6) ◽  
pp. 723 ◽  
Author(s):  
Ebrahim Cheraghi ◽  
Malek Soleimani Mehranjani ◽  
Mohammad Ali Shariatzadeh ◽  
Mohammad Hossein Nasr Esfahani ◽  
Zahra Ebrahimi
Keyword(s):  
Placebo Group ◽  
Polycystic Ovary Syndrome ◽  
Intracytoplasmic Sperm Injection ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Treated Group ◽  
Ovary Syndrome ◽  
Total Data ◽  
Oocyte Aspiration ◽  
Fluid Parameters

Polycystic ovary syndrome (PCOS) is associated with low-quality oocytes. The aim of the present study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on follicular fluid parameters, oocytes and embryo quality in PCOS patients. A prospective randomised placebo-controlled pilot study on 60 Iranian women with PCOS (aged 25–35 years) undergoing intracytoplasmic sperm injection (ICSI) was designed. Women were divided into four groups (n = 15 in each): (1) an MET, administered 1500 mg day–1 MET; (2) an NAC group, administered 1800 mg day–1 NAC; (3) an NAC + MET group; and (4) a placebo group. Drugs were administered from the 3rd day of previous cycle until the day of oocyte aspiration (6 weeks treatment in total). Data were analysed by one-way ANOVA, with significance set at P < 0.05. The number of immature and abnormal oocytes decreased significantly in the NAC compared with placebo group, with a concomitant increase in the number of good-quality embryos in the NAC group (P < 0.05). Malondialdehyde levels decreased significantly in the NAC and NAC + MET groups compared with the placebo-treated group (P < 0.02). In addition, there were significant decreases in leptin levels in the NAC, MET and NAC + MET groups compared with the placebo group (P < 0.001). Insulin and LH levels were significantly lower in the MET and NAC groups compared with the placebo-treated group (P < 0.02). We concluded that NAC improves oocyte and embryo quality and could be administered as an alternative to MET.

scholarly journals TNFR1 is Upregulated and Mediates Apoptosis in Cumulus Cells of Women With Polycystic Ovarian Syndrome

2021 ◽  
Author(s):  
Yaping Jiang ◽  
Rui Jiang ◽  
Peng Zhang ◽  
qiong Yu ◽  
Hongping Ba ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Signaling Pathway ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Control Group ◽  
Ovary Syndrome ◽  
Tnf Α ◽  
Pcos Group

Abstract Purpose To investigate the changes of human granulosa cell, TNFR1, TNFR2 and their downstream molecules in patients with polycystic ovary syndrome (PCOS) and the control group. Methods We recruited infertile women with polycystic ovary syndrome (n = 30) and compared them with infertility due to fallopian tube obstruction(n = 30, control group). The ovaries were stimulated with GnRH agonists and gonadotropins. Follicular fluid from large follicles ([14 mm]) was pooled and granulosa cells (GCs) were separated by a cellular filter. The TNF-α level of follicular fluid was measured by ELISA. TUNEL assay were used to detect the apoptosis of purified GCs. Real-time PCR and Western blotting were used to detect the expression of TNF-related signaling molecules in GCs. Results The rate of high quality embryos in the PCOS group was lower than that in the control group. There were higher percentages of apoptosis in GCs of PCOS patients than in the control group. TNF-α is upregulated in follicular fluid of PCOS patients. TNFR1 and caspase-3 mRNA level were signifificantly higher in PCOS group than in the control group. TNF-α-mediated apoptosis of PCOS granulosa cells was mainly dependent on TNFR1.The TNF-α/TNFR1 signaling pathway mediates apoptosis rather than survival in cumulus cells of PCOS patients. Conclusions TNF-α expression was upregulated in follicular fluid of PCOS patients, and TNFR1 overexpression in female granulosa cells of PCOS was associated with higher levels of apoptosis in these cells, suggesting that the TNF-α/TNFR1 signaling pathway may be a candidate for higher apoptosis in female granulosa cells of PCOS.

scholarly journals Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome

2019 ◽  
Vol 111 (6) ◽  
pp. 1243-1251.e1 ◽  
Author(s):  
Qi Che ◽  
Miao Liu ◽  
Jun Xu ◽  
Yang Liu ◽  
Xiang Cao ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Expression Profiles ◽  
Cumulus Cells ◽  
Circular Rna ◽  
Rna Expression ◽  
Ovary Syndrome
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