Prognostic impact of tumour burden measured by quantitative real-time PCR from sentinel lymph nodes of melanoma patients: data from 10-year follow-up

2016 ◽  
Vol 143 (4) ◽  
pp. 703-708 ◽  
Author(s):  
Thomas Kurt Eigentler ◽  
Joachim Hinderer ◽  
Seema Noor ◽  
Claus Garbe ◽  
Ulrike Leiter
Keyword(s):  
Lymph Nodes ◽  
Real Time ◽  
Real Time Pcr ◽  
Sentinel Lymph Nodes ◽  
Prognostic Impact ◽  
Tumour Burden ◽  
Quantitative Real Time Pcr ◽  
Melanoma Patients

The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR

2015 ◽  
Vol 141 (11) ◽  
pp. 1945-1952 ◽  
Author(s):  
Ghasem Janbabai ◽  
Ziaeddin Oladi ◽  
Touraj Farazmandfar ◽  
Tarang Taghvaei ◽  
Farshad Naghshvar
Keyword(s):  
Real Time ◽  
Gene Amplification ◽  
Real Time Pcr ◽  
Prognostic Impact ◽  
Quantitative Real Time Pcr ◽  
Gastric Carcinomas

Molecular Staging of Pathologically Negative Sentinel Lymph Nodes from Melanoma Patients Using Multimarker, Quantitative Real-Time RT-PCR

2009 ◽  
Vol 16 (1) ◽  
Author(s):  
Josep M. Hilari ◽  
Cristina Mangas ◽  
Liqiang Xi ◽  
Cristina Paradelo ◽  
Carlos Ferrándiz ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Real Time ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Molecular Staging ◽  
Melanoma Patients

Quantitative Real Time PCR Analysis of MDR1 Transduced Rhesus Macaque Hematopoietic Stem Cells Shows No Indication for Proliferative Advantage after Long-Term Follow-Up.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2198-2198
Author(s):  
Farastuk Bozorgmehr ◽  
Stephanie Laufs ◽  
Eike C. Buss ◽  
Katalina Z. Nagy ◽  
Stephanie E. Sellers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Mdr1 Gene ◽  
Quantitative Real Time Pcr ◽  
Hematopoietic Stem ◽  
Long Term Follow Up

Abstract Introduction: It is of concern whether the introduction of a transgene into hematopoietic stem cells by retroviral vectors will lead to an alteration of the growth and engraftment characteristics. Earlier studies in mice indicated that retroviral MDR1 gene transfer may be associated with a myeloproliferative disorder. In human or primate cells this could not be reproduced in bulk cell populations. Analyses on the clonal level and a long-term follow-up in a model with more direct relevance to human HSC biology were lacking. Methods: In this study CD34-enriched peripheral blood stem cells from 2 rhesus macaque monkeys were transduced either with a MDR1 gene-retroviral vector (HaMDR1-vector) or a NeoR-retroviral vector (G1Na-vector). After autologous transplantation granulocytes and mononuclear cells of each animal were obtained at different time points and analysed by using a highly sensitive and specific ligation-mediated PCR (LM-PCR) and by quantitative real time PCR. Results: In monkey M120 73 different cell populations (clones) were detected between 8 weeks and 4 years after transplantation; in monkey M038 49 clones could be identified between 16 weeks and 4 years after transplantation. Remarkably, 99 clones descend from the G1Na-vector, whereas only 23 clones descend from the HaMDR1-vector. Quantitative real time PCR analyses support these data: in both animals, the mean proportion of the G1Na-vector and the HaMDR1-vector, respectively, is 2,46%±0,014% and 0,455%±0,003%. Furthermore, single G1Na clones identified with LM-PCR 4 years after transplantation were quantified, showing a copy number between 1.43±0,52 and 7.1±0.34, according to 0.001%±0.0003% and 0.004%±0.0003% of the granulocytes fraction. In the MNC fraction, the G1Na clones were not detectable. At the same time point, 2 HaMDR1 clones were quantified, one in the granulocyte and one in the MNC fraction, respectively. Here, the copy numbers were 2.87±0.56 and 3.52±1.11, according to 0.002%±0.0003% and 0.002%±0.0008% of the granulocyte and MNC fraction, respectively. Conclusions: We conclude that hematopoiesis in these monkeys is polyclonal for prolonged periods after transplantation and that MDR1 gene transfer does not confer a proliferative advantage over vector-control-transduced hematopoietic stem cells.

Detection of breast cancer metastasis in sentinel lymph nodes using intra-operative real time PCR assay

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 11050-11050
Author(s):  
R. E. Mansel Professor ◽  
A. Goyal ◽  
A. Douglas-Jones ◽  
V. Woods ◽  
B. Jasani
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Real Time ◽  
Real Time Pcr ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Sentinel Lymph Nodes ◽  
Pcr Assay

Quantitative real-time RT-PCR in sentinel lymph nodes from melanoma patients.

Apmis ◽  
2008 ◽  
Vol 116 (3) ◽  
pp. 199-205 ◽  
Author(s):  
RIKKE RIBER-HANSEN ◽  
HELENE NORTVIG ABRAHAMSEN ◽  
BOE SANDAHL SORENSEN ◽  
STEPHEN JACQUES HAMILTON-DUTOIT ◽  
TORBEN STEINICHE
Keyword(s):  
Lymph Nodes ◽  
Real Time ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Melanoma Patients

Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR.

2002 ◽  
Vol 64 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Yoichi MOROI ◽  
Chikage OBATA ◽  
Shohei FUJITA ◽  
Satoko SHIBATA ◽  
Sawako KONISHI ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Melanoma Patients
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