Developmental rate, size, and sexual dimorphism of Megaselia scalaris (Loew) (Diptera: Phoridae): its possible implications in forensic entomology

2014 ◽  
Vol 113 (6) ◽  
pp. 2285-2294 ◽  
Author(s):  
Raja Muhammad Zuha ◽  
Baharudin Omar
Keyword(s):  
Sexual Dimorphism ◽  
Forensic Entomology ◽  
Developmental Rate ◽  
Megaselia Scalaris

Intrapuparial Development of Hemilucilia semidiaphana (Diptera: Calliphoridae) and Its Use in Forensic Entomology

2019 ◽  
Vol 56 (6) ◽  
pp. 1623-1635 ◽  
Author(s):  
Sabrina Machado da Silva ◽  
Mauricio Osvaldo Moura
Keyword(s):  
Forensic Entomology ◽  
Morphological Changes ◽  
Developmental Rate ◽  
Developmental Time ◽  
Accurate Estimate ◽  
Life Cycles ◽  
Blow Fly ◽  
Criminal Cases ◽  
Temperature Regimes ◽  
Interspecific Comparisons

Abstract In forensic entomology, the minimum postmortem interval (PMImin) estimative is usually based on the oldest immature recovered from a local of death. The time spent by fly immatures in the intrapuparial period comprises more than 50% of their complete life cycles. An accurate estimate of the duration of this period will improve PMImin estimates. The blow fly Hemilucilia semidiaphana (Rondani 1850) was found in six criminal cases in the city of Curitiba. Even though there is data on the morphology of the larval instars and developmental rate of H. semidiaphana, the intrapuparial period has not been investigated. Here, we provide a detailed description of the intrapuparial morphological changes of H. semidiaphana, which might be useful to estimate minimum PMI. Samples of H. semidiaphana in the intrapuparial period were obtained from immatures reared on an artificial diet in incubators adjusted to 25°C or 20°C temperature regimes. Blow fly puparia of H. semidiaphana were fixed at intervals of 3 and 6 h until emergence of the adult. The external morphological traits of sampled immatures were analyzed using light microscopy. Our analysis of the intrapuparial period of H. semidiaphana provided 21 traits from which nine were age informative. These nine characteristics divide the developmental time (144 h at 25°C and 192 h at 20°C) into smaller sections. The developmental data provided, together with the time line allows a practical way to make interspecific comparisons as well as to estimate the age of H. semidiaphana based on the intrapuparial development.

Developmental Biology of Forensically Important Beetle, Necrophila (Calosilpha) brunnicollis (Coleoptera: Silphidae)

2020 ◽  
Author(s):  
Pavel Jakubec ◽  
Jarin Qubaiová ◽  
Martin Novák ◽  
Jan Růžička
Keyword(s):  
Sexual Dimorphism ◽  
Developmental Stages ◽  
Larval Instar ◽  
Developmental Rate ◽  
The Body ◽  
Important Species ◽  
Developmental Thresholds ◽  
Sum Of Effective Temperatures ◽  
Carrion Beetle ◽  
Pmi Estimation

Abstract The postmortem interval (PMI) estimation, in cases where the body was discovered in an advanced stage of decomposition, is predominantly based on entomological evidence. However, very few forensically important species are sufficiently known in detail to allow a practical application. One of them is the carrion beetle, Necrophila (Calosilpha) brunnicollis (Kraatz, 1877). Its development from egg to adulthood was studied under a range of ecologically relevant constant temperatures to find parameters of thermal summation models. Developmental sexual dimorphism and the presence of developmental rate isomorphy were investigated. Herein we present the lower developmental thresholds and sum of effective temperatures for all developmental stages of N. brunnicollis (egg, first–third larval instar, postfeeding stage, and pupae). We did not find any evidence of developmental sexual dimorphism nor was the presence of developmental rate isomorphy confirmed. Our results present the first thermal summation model of the East Asian carrion beetle that can be used for the PMI estimation.

Sexual dimorphism in developmental rate and ecdysteroid titre in Orgyia postica

1992 ◽  
Vol 38 (12) ◽  
pp. 1043-1049 ◽  
Author(s):  
Shi-Hong Gu ◽  
Ru-Shiow Tsai ◽  
Yien-Shing Chow ◽  
Fei-Jann Lin
Keyword(s):  
Sexual Dimorphism ◽  
Developmental Rate

Morphology and developmental rate of blowflies Chrysomya megacephala and Chrysomya rufifacies in Thailand: application in forensic entomology

2008 ◽  
Vol 102 (6) ◽  
pp. 1207-1216 ◽  
Author(s):  
Kom Sukontason ◽  
Somsak Piangjai ◽  
Sirisuda Siriwattanarungsee ◽  
Kabkaew L. Sukontason
Keyword(s):  
Forensic Entomology ◽  
Developmental Rate ◽  
Chrysomya Rufifacies ◽  
Chrysomya Megacephala

scholarly journals Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5962 ◽  
Author(s):  
Sara Bortolini ◽  
Giorgia Giordani ◽  
Fabiola Tuccia ◽  
Lara Maistrello ◽  
Stefano Vanin
Keyword(s):  
Forensic Entomology ◽  
Unsolved Problem ◽  
Phormia Regina ◽  
Calliphora Vicina ◽  
Morphological Identification ◽  
Correct Identification ◽  
Closely Related Species ◽  
Crucial Step ◽  
Post Mortem Interval ◽  
Megaselia Scalaris

Species identification is a crucial step in forensic entomology. In several cases the calculation of the larval age allows the estimation of the minimum Post-Mortem Interval (mPMI). A correct identification of the species is the first step for a correct mPMI estimation. To overcome the difficulties due to the morphological identification especially of the immature stages, a molecular approach can be applied. However, difficulties in separation of closely related species are still an unsolved problem. Sequences of 4 different genes (COI, ND5, EF-1α, PER) of 13 different fly species collected during forensic experiments (Calliphora vicina, Calliphora vomitoria, Lucilia sericata, Lucilia illustris, Lucilia caesar, Chrysomya albiceps, Phormia regina, Cynomya mortuorum, Sarcophaga sp., Hydrotaea sp., Fannia scalaris, Piophila sp., Megaselia scalaris) were evaluated for their capability to identify correctly the species. Three concatenated sequences were obtained combining the four genes in order to verify if longer sequences increase the probability of a correct identification. The obtained results showed that this rule does not work for the species L. caesar and L. illustris. Future works on other DNA regions are suggested to solve this taxonomic issue.

scholarly journals Morphology and Developmental Rate of the Blow Fly,Hemipyrellia ligurriens(Diptera: Calliphoridae): Forensic Entomology Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Nophawan Bunchu ◽  
Chinnapat Thaipakdee ◽  
Apichat Vitta ◽  
Sangob Sanit ◽  
Kom Sukontason ◽  
...  
Keyword(s):  
Light Microscope ◽  
Forensic Entomology ◽  
Development Time ◽  
Developmental Rate ◽  
Developmental Time ◽  
Morphological Features ◽  
Forensic Investigation ◽  
Blow Fly ◽  
Ambient Conditions ◽  
Complete Metamorphosis

Hemipyrellia ligurriens(Diptera: Calliphoridae) is a forensically important blow fly species presented in many countries. In this study, we determined the morphology of all stages and the developmental rate ofH. ligurriensreared under natural ambient conditions in Phitsanulok province, northern Thailand. Morphological features of all stages based on observing under a light microscope were described and demonstrated in order to use for identification purpose. Moreover, development time in each stage was given. The developmental time ofH. ligurriensto complete metamorphosis; from egg, larva, pupa to adult, took 270.71 h for 1 cycle of development. The results from this study may be useful not only for application in forensic investigation, but also for study in its biology in the future.

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